| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,943,161 | A→G | 5.9% | K78E (AAG→GAG) ‡ | bglA → | 6‑phospho‑beta‑glucosidase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,943,161 | 0 | A | G | 5.9% | 193.3 / 2.5 | 67 | K78E (AAG→GAG) ‡ | bglA | 6‑phospho‑beta‑glucosidase A |
| Reads supporting (aligned to +/- strand): ref base A (29/34); new base G (2/2); total (31/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
GCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGG > Exported/1943097‑1943231 | gCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCt < 1:2063208/69‑1 (MQ=255)gCCAGGAAAATACTATCCAAACCATGAAGCCATTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTa > 1:4559184/1‑70 (MQ=255) ccAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTa < 1:916333/69‑1 (MQ=255) ccAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTAt < 1:2964851/70‑1 (MQ=255) cAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTAttt < 1:5597187/71‑1 (MQ=255) aGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTg > 1:666073/1‑71 (MQ=255) ggAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGc < 1:5199738/71‑1 (MQ=255) aaaTACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTAtt > 1:4103314/1‑65 (MQ=255) aaaTACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGa < 1:1215014/71‑1 (MQ=255) aaaTACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGa < 1:2714342/71‑1 (MQ=255) aaaTACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGa < 1:5507287/71‑1 (MQ=255) aTACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGaaa > 1:4811708/1‑71 (MQ=255) aCTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATg > 1:5543989/1‑71 (MQ=255) cTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATgg < 1:21432/71‑1 (MQ=255) cTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATg < 1:2554015/70‑1 (MQ=255) cAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCtt < 1:1933464/70‑1 (MQ=255) cAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCtt > 1:589269/1‑70 (MQ=255) aaCCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCtt < 1:3115914/68‑1 (MQ=255) aaCCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCaa < 1:4409780/71‑1 (MQ=255) aaCCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCa > 1:869867/1‑70 (MQ=255) ccATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAAt > 1:2176368/1‑71 (MQ=255) ccATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAAt > 1:5057066/1‑71 (MQ=255) tGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCt < 1:438776/45‑1 (MQ=255) tGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTAt > 1:967911/1‑47 (MQ=255) aaGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGttt < 1:1281601/70‑1 (MQ=255) aaGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGtt > 1:4994179/1‑69 (MQ=255) gCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCg > 1:5194858/1‑71 (MQ=255) gCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCg > 1:5188412/1‑71 (MQ=255) gTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTAc < 1:1646768/71‑1 (MQ=255) ttGATTTTTATGGTCACTATAAGGAAGACATCAAGCTAt < 1:15386/39‑1 (MQ=255) ttGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGa < 1:472836/46‑1 (MQ=255) ttGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGa < 1:3593195/46‑1 (MQ=255) ttGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGtttt < 1:2610467/65‑1 (MQ=255) tGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGa < 1:1574393/45‑1 (MQ=255) gATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGcc > 1:2196286/1‑42 (MQ=255) gATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGtt > 1:2394812/1‑61 (MQ=255) gATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTa > 1:1758445/1‑67 (MQ=255) gATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc > 1:4981092/1‑71 (MQ=255) aTTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTg < 1:3607882/39‑1 (MQ=255) aTTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATggg > 1:3871363/1‑49 (MQ=255) ttttATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCAt > 1:2646213/1‑71 (MQ=255) aTGGTCACTATAAGGAAGACATCAAGCTATTTGCCGaaa > 1:529061/1‑39 (MQ=255) gTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTg < 1:543105/70‑1 (MQ=255) tataAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACAt < 1:495589/55‑1 (MQ=255) ataAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCa > 1:3619433/1‑57 (MQ=255) ataAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGAcgc > 1:2506636/1‑69 (MQ=255) aGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTc > 1:4256618/1‑45 (MQ=255) aGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAtt > 1:3264055/1‑71 (MQ=255) aGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAtt > 1:253814/1‑71 (MQ=255) ggAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAttt < 1:1477950/71‑1 (MQ=255) ggAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAttt > 1:764619/1‑71 (MQ=255) ggAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGACGCGAAttt < 1:2554187/71‑1 (MQ=255) ggAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc < 1:2639763/51‑1 (MQ=255) ggAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc < 1:4451693/51‑1 (MQ=255) gAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAtttt > 1:532169/1‑71 (MQ=255) gAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGtttt > 1:3709977/1‑42 (MQ=255) aaGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACAt < 1:1327729/48‑1 (MQ=255) aaGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttttt > 1:2585227/1‑71 (MQ=255) aCATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACgcgc < 1:3753628/62‑1 (MQ=255) cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa < 1:1202158/71‑1 (MQ=255) cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa < 1:3915062/71‑1 (MQ=255) cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa < 1:2737300/71‑1 (MQ=255) cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa < 1:1660478/71‑1 (MQ=255) tCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaaa < 1:2251707/71‑1 (MQ=255) cAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc < 1:3208253/42‑1 (MQ=255) cAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaa > 1:5078748/1‑69 (MQ=255) cAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaa > 1:1561382/1‑69 (MQ=255) aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa > 1:2398283/1‑52 (MQ=255) aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa > 1:1521333/1‑52 (MQ=255) aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa > 1:1166436/1‑52 (MQ=255) aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAgg > 1:5079192/1‑71 (MQ=255) aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAgg > 1:3382894/1‑71 (MQ=255) aaGCTATTTGCCAAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa > 1:2704324/1‑52 (MQ=255) | GCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGG > Exported/1943097‑1943231 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |