| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,943,175 | A→G | 25.8% | E82E (GAA→GAG) | bglA → | 6‑phospho‑beta‑glucosidase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,943,175 | 0 | A | G | 25.8% | 138.8 / 54.2 | 89 | E82E (GAA→GAG) | bglA | 6‑phospho‑beta‑glucosidase A |
| Reads supporting (aligned to +/- strand): ref base A (33/33); new base G (9/14); total (42/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
ATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGC > Exported/1943106‑1943245 | aTACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGaaa > 1:4811708/1‑71 (MQ=255) aCTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATg > 1:5543989/1‑71 (MQ=255) cTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATgg < 1:21432/71‑1 (MQ=255) cTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATg < 1:2554015/70‑1 (MQ=255) cAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCtt > 1:589269/1‑70 (MQ=255) cAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCtt < 1:1933464/70‑1 (MQ=255) aaCCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCtt < 1:3115914/68‑1 (MQ=255) aaCCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCaa < 1:4409780/71‑1 (MQ=255) aaCCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCa > 1:869867/1‑70 (MQ=255) ccATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAAt > 1:5057066/1‑71 (MQ=255) ccATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAAt > 1:2176368/1‑71 (MQ=255) aaGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGttt < 1:1281601/70‑1 (MQ=255) aaGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGtt > 1:4994179/1‑69 (MQ=255) gCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCg > 1:5194858/1‑71 (MQ=255) gCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCg > 1:5188412/1‑71 (MQ=255) gTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTAc < 1:1646768/71‑1 (MQ=255) ttGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGtttt < 1:2610467/65‑1 (MQ=255) gATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGtt > 1:2394812/1‑61 (MQ=255) gATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTa > 1:1758445/1‑67 (MQ=255) gATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc > 1:4981092/1‑71 (MQ=255) aTTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATggg > 1:3871363/1‑49 (MQ=255) ttttATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCAt > 1:2646213/1‑71 (MQ=255) aTGGTCACTATAAGGAAGACATCAAGCTATTTGCCGaaa > 1:529061/1‑39 (MQ=255) gTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTg < 1:543105/70‑1 (MQ=255) tataAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACAt < 1:495589/55‑1 (MQ=255) ataAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCa > 1:3619433/1‑57 (MQ=255) ataAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGAcgc > 1:2506636/1‑69 (MQ=255) aGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTc > 1:4256618/1‑45 (MQ=255) aGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAtt > 1:3264055/1‑71 (MQ=255) aGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAtt > 1:253814/1‑71 (MQ=255) ggAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAttt < 1:1477950/71‑1 (MQ=255) ggAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAttt > 1:764619/1‑71 (MQ=255) ggAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGACGCGAAttt < 1:2554187/71‑1 (MQ=255) ggAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc < 1:4451693/51‑1 (MQ=255) ggAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc < 1:2639763/51‑1 (MQ=255) gAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAtttt > 1:532169/1‑71 (MQ=255) gAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGtttt > 1:3709977/1‑42 (MQ=255) aaGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACAt < 1:1327729/48‑1 (MQ=255) aaGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttttt > 1:2585227/1‑71 (MQ=255) aCATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACgcgc < 1:3753628/62‑1 (MQ=255) cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa < 1:1202158/71‑1 (MQ=255) cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa < 1:2737300/71‑1 (MQ=255) cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa < 1:3915062/71‑1 (MQ=255) cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa < 1:1660478/71‑1 (MQ=255) tCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaaa < 1:2251707/71‑1 (MQ=255) cAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc < 1:3208253/42‑1 (MQ=255) cAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaa > 1:5078748/1‑69 (MQ=255) cAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaa > 1:1561382/1‑69 (MQ=255) aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa > 1:1166436/1‑52 (MQ=255) aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa > 1:1521333/1‑52 (MQ=255) aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa > 1:2398283/1‑52 (MQ=255) aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAgg > 1:3382894/1‑71 (MQ=255) aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAgg > 1:5079192/1‑71 (MQ=255) aaGCTATTTGCCAAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa > 1:2704324/1‑52 (MQ=255) ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGc < 1:2787904/43‑1 (MQ=255) ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCt < 1:1014966/45‑1 (MQ=255) ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCt < 1:1751890/45‑1 (MQ=255) ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCt < 1:2433945/45‑1 (MQ=255) ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCt < 1:2951430/45‑1 (MQ=255) ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCt < 1:2860158/45‑1 (MQ=255) ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTg > 1:2727003/2‑47 (MQ=255) ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTg > 1:2378438/2‑47 (MQ=255) tATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc < 1:2929240/37‑1 (MQ=255) aTTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGac > 1:2223538/1‑69 (MQ=255) aTTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGAt > 1:2868506/1‑70 (MQ=255) tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAtttt < 1:4656174/56‑1 (MQ=255) tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGacg > 1:2557695/1‑68 (MQ=255) tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGac > 1:5424863/1‑68 (MQ=255) tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGac > 1:5418485/1‑68 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt < 1:5500840/55‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt < 1:5487623/55‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTcc < 1:5689610/59‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg < 1:3887882/70‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg < 1:2761226/70‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg < 1:782863/70‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGa < 1:2378942/71‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGa < 1:1408563/71‑1 (MQ=255) tGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa < 1:373058/70‑1 (MQ=255) tGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa < 1:1182698/70‑1 (MQ=255) tGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGa > 1:2888843/1‑66 (MQ=255) tGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAg > 1:5497366/1‑71 (MQ=255) gCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAtt > 1:1556532/1‑51 (MQ=255) ccGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa < 1:5681630/68‑1 (MQ=255) ccGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa < 1:3346741/68‑1 (MQ=255) ccGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGAAgtc < 1:1774428/71‑3 (MQ=255) ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGAt < 1:4395735/65‑1 (MQ=255) ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGAt < 1:4237026/65‑1 (MQ=255) ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGc < 1:3439558/70‑1 (MQ=255) ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt < 1:132510/71‑1 (MQ=255) gAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt > 1:1439941/1‑69 (MQ=255) gAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt > 1:3975968/1‑69 (MQ=255) aaTGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGc > 1:3092252/1‑71 (MQ=255) | ATACTATCCAAACCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGC > Exported/1943106‑1943245 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |