Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,943,212 A→G 12.6% T95A (ACG→GCG)  bglA → 6‑phospho‑beta‑glucosidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,943,2120AG12.6% 292.2 / 28.6 119T95A (ACG→GCG) bglA6‑phospho‑beta‑glucosidase A
Reads supporting (aligned to +/- strand):  ref base A (55/49);  new base G (7/8);  total (62/57)
Fisher's exact test for biased strand distribution p-value = 7.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

Only 100 of 124 total aligned reads displayed.

ATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTT  >  Exported/1943147‑1943282
                                                                 |                                                                      
ataAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGAcgc                                                                     >  1:2506636/1‑69 (MQ=255)
   aGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAtt                                                                >  1:253814/1‑71 (MQ=255)
   aGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAtt                                                                >  1:3264055/1‑71 (MQ=255)
    ggAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAttt                                                               >  1:764619/1‑71 (MQ=255)
    ggAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGACGCGAAttt                                                               <  1:2554187/71‑1 (MQ=255)
      aaGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttttt                                                             >  1:2585227/1‑71 (MQ=255)
         aCATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACgcgc                                                                   <  1:3753628/62‑1 (MQ=255)
          cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa                                                         <  1:1202158/71‑1 (MQ=255)
          cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa                                                         <  1:3915062/71‑1 (MQ=255)
          cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa                                                         <  1:1660478/71‑1 (MQ=255)
            tCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaaa                                                       <  1:2251707/71‑1 (MQ=255)
             cAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaa                                                        >  1:5078748/1‑69 (MQ=255)
             cAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaa                                                        >  1:1561382/1‑69 (MQ=255)
              aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa                                                                        >  1:1521333/1‑52 (MQ=255)
              aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa                                                                        >  1:1166436/1‑52 (MQ=255)
              aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAgg                                                     >  1:3382894/1‑71 (MQ=255)
              aaGCTATTTGCCAAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa                                                                        >  1:2704324/1‑52 (MQ=255)
                   aTTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGac                                                 >  1:2223538/1‑69 (MQ=255)
                   aTTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGAt                                                 >  1:2868506/1‑70 (MQ=255)
                    tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAtttt                                                              <  1:4656174/56‑1 (MQ=255)
                    tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGac                                                 >  1:5424863/1‑68 (MQ=255)
                    tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt                                                               <  1:5487623/55‑1 (MQ=255)
                    tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt                                                               <  1:5500840/55‑1 (MQ=255)
                    tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTcc                                                           <  1:5689610/59‑1 (MQ=255)
                    tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg                                                <  1:3887882/70‑1 (MQ=255)
                    tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg                                                <  1:782863/70‑1 (MQ=255)
                    tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg                                                <  1:2761226/70‑1 (MQ=255)
                    tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGa                                               <  1:2378942/71‑1 (MQ=255)
                    tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGa                                               <  1:1408563/71‑1 (MQ=255)
                      tGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa                                              <  1:373058/70‑1 (MQ=255)
                      tGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa                                              <  1:1182698/70‑1 (MQ=255)
                      tGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGa                                                  >  1:2888843/1‑66 (MQ=255)
                      tGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAg                                             >  1:5497366/1‑71 (MQ=255)
                       gCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAtt                                                                >  1:1556532/1‑51 (MQ=255)
                        ccGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa                                              <  1:5681630/68‑1 (MQ=255)
                        ccGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGAAgtc                                           <  1:1774428/71‑3 (MQ=255)
                        ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGAt                                                 <  1:4237026/65‑1 (MQ=255)
                        ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGc                                            <  1:3439558/70‑1 (MQ=255)
                        ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt                                           <  1:132510/71‑1 (MQ=255)
                          gAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt                                           >  1:1439941/1‑69 (MQ=255)
                          gAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt                                           >  1:3975968/1‑69 (MQ=255)
                            aaTGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGc                                       >  1:3092252/1‑71 (MQ=255)
                              tGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGcgacga                                               <  1:2414136/61‑1 (MQ=255)
                               gggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCa                                     >  1:4804842/1‑70 (MQ=255)
                               gggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCa                                     >  1:40758/1‑70 (MQ=255)
                                ggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaa                                    <  1:3531599/70‑1 (MQ=255)
                                 gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg                                                <  1:4422594/57‑1 (MQ=255)
                                 gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg                                                <  1:5446878/57‑1 (MQ=255)
                                 gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaa                                    <  1:5023357/69‑1 (MQ=255)
                                 gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCACATTTTTCCAAAAGGCGATg                                                <  1:1586141/57‑1 (MQ=255)
                                  cTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt                                                               <  1:293134/41‑1 (MQ=255)
                                  cTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt                                                               <  1:4405322/41‑1 (MQ=255)
                                    tCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTcc                                                           <  1:5401820/43‑1 (MQ=255)
                                    tCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTcc                                                           <  1:5015099/43‑1 (MQ=255)
                                     cAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAgg                                                     >  1:3078846/1‑48 (MQ=255)
                                      aaaTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACgaag                              <  1:3468139/70‑1 (MQ=255)
                                       aaTGTTTTCGTACATCCATTGCCTGGAGGCGCATTTTTCCAAAAGGCGATGAAGCTCAGcc                                      >  1:3087242/1‑61 (MQ=255)
                                        aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTc                                          >  1:4532978/1‑56 (MQ=255)
                                        aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGaagaag                           >  1:54746/1‑71 (MQ=255)
                                        aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGaagaag                           >  1:2320890/1‑71 (MQ=255)
                                          gTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGa                                                  >  1:3968923/1‑46 (MQ=255)
                                          gTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAggg                         >  1:828286/1‑71 (MQ=255)
                                          gTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAgg                          >  1:5163031/1‑70 (MQ=255)
                                           ttttCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTc                                          <  1:4460066/53‑1 (MQ=255)
                                              tCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt                                           <  1:5515062/49‑1 (MQ=255)
                                              tCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTg                      >  1:2610421/1‑70 (MQ=255)
                                              tCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTg                      >  1:452740/1‑70 (MQ=255)
                                              tCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTg                      <  1:4410441/70‑1 (MQ=255)
                                                 tACATCCATTGCCTGGACGCGCATTTTTACAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGt                  <  1:5561714/71‑1 (MQ=255)
                                                  aCATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCg                                                   <  1:5414125/37‑1 (MQ=255)
                                                  aCATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGaa                    >  1:3119779/1‑68 (MQ=255)
                                                  aCATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGtt                 >  1:35992/1‑71 (MQ=255)
                                                   cATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGttg                <  1:3918682/71‑2 (MQ=255)
                                                   cATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTc                >  1:4727373/1‑71 (MQ=255)
                                                   cATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTc                >  1:5297251/1‑71 (MQ=255)
                                                   cATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTc                <  1:410016/71‑1 (MQ=255)
                                                     tCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACgaaga                             >  1:4877372/1‑56 (MQ=255)
                                                     tCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACgaaga                             >  1:5644705/1‑56 (MQ=255)
                                                      ccATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGc                                            <  1:5413390/40‑1 (MQ=255)
                                                      ccATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAggg                         >  1:1128385/1‑59 (MQ=255)
                                                      ccATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc             >  1:1567920/1‑71 (MQ=255)
                                                       cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt                                           >  1:1939601/1‑40 (MQ=255)
                                                       cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc             >  1:87697/1‑70 (MQ=255)
                                                       cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc             >  1:5328887/1‑70 (MQ=255)
                                                       cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc             <  1:1387380/70‑1 (MQ=255)
                                                       cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc             <  1:4206074/70‑1 (MQ=255)
                                                       cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACg            <  1:520970/71‑1 (MQ=255)
                                                       cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAACGGCTGAAGTTCTa              >  1:4928409/1‑69 (MQ=255)
                                                         ttGCCTGGACGCGCATTTTTCCAAAAGGCTATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc             <  1:2714317/68‑1 (MQ=255)
                                                          tGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGaa                    >  1:3207257/1‑60 (MQ=255)
                                                          tGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACgat          >  1:3278914/1‑70 (MQ=255)
                                                            ccTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACgatga        <  1:3718369/70‑1 (MQ=255)
                                                              tGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc             <  1:4498266/63‑1 (MQ=255)
                                                              tGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc             <  1:2602028/63‑1 (MQ=255)
                                                               ggACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTa              >  1:527416/1‑61 (MQ=255)
                                                               ggACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTa              >  1:1129873/1‑61 (MQ=255)
                                                                gACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaaa                                   >  1:44252/1‑39 (MQ=255)
                                                                gACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGatat     >  1:5517412/1‑69 (MQ=255)
                                                                gACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGt   >  1:3306118/1‑71 (MQ=255)
                                                                 aCGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGtt  <  1:5131945/71‑1 (MQ=255)
                                                                 |                                                                      
ATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTT  >  Exported/1943147‑1943282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑