Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,943,217 C→A 13.1% R96R (CGC→CGA)  bglA → 6‑phospho‑beta‑glucosidase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,943,2170CA13.1% 360.4 / 25.5 122R96R (CGC→CGA) bglA6‑phospho‑beta‑glucosidase A
Reads supporting (aligned to +/- strand):  ref base C (56/50);  new base A (7/9);  total (63/59)
Fisher's exact test for biased strand distribution p-value = 5.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

Only 100 of 128 total aligned reads displayed.

AGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATG  >  Exported/1943150‑1943287
                                                                   |                                                                      
aGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAtt                                                                     >  1:3264055/1‑71 (MQ=255)
aGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAtt                                                                     >  1:253814/1‑71 (MQ=255)
 ggAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAttt                                                                    <  1:1477950/71‑1 (MQ=255)
   aaGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttttt                                                                  >  1:2585227/1‑71 (MQ=255)
       cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa                                                              <  1:2737300/71‑1 (MQ=255)
       cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa                                                              <  1:3915062/71‑1 (MQ=255)
       cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa                                                              <  1:1660478/71‑1 (MQ=255)
          cAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaa                                                             >  1:5078748/1‑69 (MQ=255)
          cAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaa                                                             >  1:1561382/1‑69 (MQ=255)
           aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAgg                                                          >  1:5079192/1‑71 (MQ=255)
           aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAgg                                                          >  1:3382894/1‑71 (MQ=255)
                aTTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGac                                                      >  1:2223538/1‑69 (MQ=255)
                aTTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGAt                                                      >  1:2868506/1‑70 (MQ=255)
                 tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAtttt                                                                   <  1:4656174/56‑1 (MQ=255)
                 tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGacg                                                     >  1:2557695/1‑68 (MQ=255)
                 tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGac                                                      >  1:5424863/1‑68 (MQ=255)
                 tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt                                                                    <  1:5500840/55‑1 (MQ=255)
                 tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTcc                                                                <  1:5689610/59‑1 (MQ=255)
                 tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg                                                     <  1:3887882/70‑1 (MQ=255)
                 tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg                                                     <  1:2761226/70‑1 (MQ=255)
                 tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg                                                     <  1:782863/70‑1 (MQ=255)
                 tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGa                                                    <  1:2378942/71‑1 (MQ=255)
                 tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGa                                                    <  1:1408563/71‑1 (MQ=255)
                   tGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa                                                   <  1:373058/70‑1 (MQ=255)
                   tGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa                                                   <  1:1182698/70‑1 (MQ=255)
                   tGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAg                                                  >  1:5497366/1‑71 (MQ=255)
                    gCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAtt                                                                     >  1:1556532/1‑51 (MQ=255)
                     ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGAt                                                      <  1:4395735/65‑1 (MQ=255)
                     ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGAt                                                      <  1:4237026/65‑1 (MQ=255)
                     ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGc                                                 <  1:3439558/70‑1 (MQ=255)
                     ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt                                                <  1:132510/71‑1 (MQ=255)
                       gAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt                                                >  1:3975968/1‑69 (MQ=255)
                         aaTGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGc                                            >  1:3092252/1‑71 (MQ=255)
                           tGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGcgacga                                                    <  1:2414136/61‑1 (MQ=255)
                            gggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCa                                          >  1:4804842/1‑70 (MQ=255)
                            gggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCa                                          >  1:40758/1‑70 (MQ=255)
                             ggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaa                                         <  1:3538224/70‑1 (MQ=255)
                              gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg                                                     <  1:4422594/57‑1 (MQ=255)
                              gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg                                                     <  1:5446878/57‑1 (MQ=255)
                              gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaa                                         <  1:5023357/69‑1 (MQ=255)
                              gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCACATTTTTCCAAAAGGCGATg                                                     <  1:1586141/57‑1 (MQ=255)
                               cTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt                                                                    <  1:4405322/41‑1 (MQ=255)
                               cTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt                                                                    <  1:293134/41‑1 (MQ=255)
                               cTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt                                                                    <  1:2040899/41‑1 (MQ=255)
                                 tCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTcc                                                                <  1:5401820/43‑1 (MQ=255)
                                  cAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAgg                                                          >  1:3078846/1‑48 (MQ=255)
                                  cAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACg                                      >  1:4576113/1‑68 (MQ=255)
                                   aaaTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACgaag                                   <  1:3468139/70‑1 (MQ=255)
                                    aaTGTTCTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAg                                                           >  1:12434/1‑45 (MQ=255)
                                     aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTc                                               >  1:4532978/1‑56 (MQ=255)
                                     aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTc                                               >  1:757194/1‑56 (MQ=255)
                                     aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGaagaag                                >  1:2320890/1‑71 (MQ=255)
                                     aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGaagaag                                >  1:54746/1‑71 (MQ=255)
                                      tGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAgg                               >  1:5491372/1‑71 (MQ=255)
                                       gTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGa                                                       >  1:4443691/1‑46 (MQ=255)
                                       gTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGa                                                       >  1:3968923/1‑46 (MQ=255)
                                       gTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAggg                              >  1:828286/1‑71 (MQ=255)
                                       gTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAgg                               >  1:5163031/1‑70 (MQ=255)
                                         tttCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCt                            >  1:5520228/1‑71 (MQ=255)
                                           tCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt                                                <  1:5515062/49‑1 (MQ=255)
                                           tCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTg                           >  1:452740/1‑70 (MQ=255)
                                              tACATCCATTGCCTGGACGCGCATTTTTACAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGt                       <  1:5561714/71‑1 (MQ=255)
                                               aCATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCg                                                        <  1:5414125/37‑1 (MQ=255)
                                               aCATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCg                                                        <  1:3188849/37‑1 (MQ=255)
                                               aCATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGaa                         >  1:3119779/1‑68 (MQ=255)
                                               aCATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGtt                      >  1:35992/1‑71 (MQ=255)
                                               aCATCCATTGCCTGGACGCGCATTTATCCAAAAGGCg                                                        <  1:3012323/37‑1 (MQ=255)
                                                cATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACgaaga                                  <  1:3792148/58‑1 (MQ=255)
                                                cATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTc                     >  1:4727373/1‑71 (MQ=255)
                                                cATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTc                     >  1:5297251/1‑71 (MQ=255)
                                                  tCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACgaaga                                  >  1:5644705/1‑56 (MQ=255)
                                                   ccATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGc                                                 <  1:5413390/40‑1 (MQ=255)
                                                   ccATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAggg                              >  1:1128385/1‑59 (MQ=255)
                                                    cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt                                                >  1:1939601/1‑40 (MQ=255)
                                                    cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc                  >  1:5328887/1‑70 (MQ=255)
                                                    cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc                  >  1:87697/1‑70 (MQ=255)
                                                    cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc                  <  1:1387380/70‑1 (MQ=255)
                                                    cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc                  <  1:4206074/70‑1 (MQ=255)
                                                    cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACg                 <  1:520970/71‑1 (MQ=255)
                                                    cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAACGGCTGAAGTTCTa                   >  1:4928409/1‑69 (MQ=255)
                                                      ttGCCTGGACGCGCATTTTTCCAAAAGGCTATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc                  <  1:2714317/68‑1 (MQ=255)
                                                       tGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACgat               >  1:3278914/1‑70 (MQ=255)
                                                        gCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACgatga             >  1:1582035/1‑71 (MQ=255)
                                                         ccTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACgatga             <  1:3718369/70‑1 (MQ=255)
                                                           tGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc                  <  1:4498266/63‑1 (MQ=255)
                                                           tGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc                  <  1:2602028/63‑1 (MQ=255)
                                                            ggACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTa                   >  1:527416/1‑61 (MQ=255)
                                                            ggACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTa                   >  1:1129873/1‑61 (MQ=255)
                                                            ggACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATg         >  1:489374/1‑71 (MQ=255)
                                                             gACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaaa                                        >  1:406420/1‑39 (MQ=255)
                                                             gACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaaa                                        >  1:44252/1‑39 (MQ=255)
                                                             gACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGt        >  1:3306118/1‑71 (MQ=255)
                                                               cgcgCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTc      <  1:3287107/71‑1 (MQ=255)
                                                               cgcgCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTc      >  1:1742485/1‑71 (MQ=255)
                                                               cgcgCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTc      <  1:1179960/71‑1 (MQ=255)
                                                               cgcgCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTc      >  1:5013462/1‑71 (MQ=255)
                                                                 cgcATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGa    <  1:3036843/71‑1 (MQ=255)
                                                                  gcATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGaa                         >  1:5697141/1‑49 (MQ=255)
                                                                  gcATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGaa                         >  1:720485/1‑49 (MQ=255)
                                                                   cATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATg  <  1:4988778/71‑1 (MQ=255)
                                                                   |                                                                      
AGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATG  >  Exported/1943150‑1943287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑