Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,960,131 T→A 8.1% intergenic (‑61/+154) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,960,1310TA 8.1% 166.2 / 6.4 62intergenic (‑61/+154)fbaA/pgkfructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
Reads supporting (aligned to +/- strand):  ref base T (23/34);  new base A (2/3);  total (25/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

TTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTG  >  Exported/1960063‑1960200
                                                                    |                                                                     
ttAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGctgcgtct                                                                     >  1:3240759/1‑71 (MQ=255)
  aGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGctgcgtct                                                                     <  1:4551398/69‑1 (MQ=255)
  aGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGctgcgtct                                                                     <  1:3031966/69‑1 (MQ=255)
  aGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGctgcgtct                                                                     <  1:5098834/69‑1 (MQ=255)
      aTGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTc                                                               <  1:5057075/71‑1 (MQ=255)
       tGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCg                                                              <  1:4487668/71‑1 (MQ=255)
         tctcTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTc                                                               >  1:3605422/1‑68 (MQ=255)
         tctcTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGc                                                             >  1:4345179/1‑70 (MQ=255)
             ttGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAc                                                         >  1:1222317/1‑70 (MQ=255)
                tCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGctgcgtct                                                                     <  1:2470581/55‑1 (MQ=255)
                tCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAcccc                                                      <  1:2000131/70‑1 (MQ=255)
                  cTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGctgcgtctgc                                                                   <  1:245556/55‑1 (MQ=255)
                  cTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAg                                                    <  1:386494/70‑1 (MQ=255)
                   tGTATCGTCGGGCCTTATACTCGTCATAATTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGt                                                   >  1:1953895/1‑70 (MQ=255)
                     tATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTc                                                  <  1:1731547/69‑1 (MQ=255)
                     tATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTc                                                  <  1:823117/69‑1 (MQ=255)
                      aTCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGctgcgtc                                                                      >  1:4465177/1‑48 (MQ=255)
                      aTCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGctgcgtc                                                                      >  1:1222256/1‑48 (MQ=255)
                      aTCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGctgcgtc                                                                      >  1:5067897/1‑48 (MQ=255)
                      aTCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCact                                               >  1:4368108/1‑71 (MQ=255)
                         gtcgGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc                                                       >  1:1786028/1‑60 (MQ=255)
                         gtcgGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCacttac                                            <  1:5616523/71‑1 (MQ=255)
                           cgGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCacttact                                           >  1:2788413/1‑70 (MQ=255)
                           cgGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCacttact                                           >  1:544825/1‑70 (MQ=255)
                            gggCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCActtactta                                         <  1:5500354/71‑1 (MQ=255)
                            gggCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCActtactta                                         <  1:407403/71‑1 (MQ=255)
                            gggCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCActtactta                                         >  1:1811834/1‑71 (MQ=255)
                            gggCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCActtactta                                         <  1:2099354/71‑1 (MQ=255)
                              gCCTTATACTCGTCATACTTCAAGTTGCATGTGctgcgtct                                                                     <  1:5548751/41‑1 (MQ=255)
                              gCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTAt                                        <  1:5280998/70‑1 (MQ=255)
                              catttATACTCGTCATACTTCAAGTTGCATGTACTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTAt                                        <  1:979494/67‑1 (MQ=11)
                              catttATACTCGTCATACTTCAAGTTGCATGTACTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATg                                       <  1:1370932/68‑1 (MQ=11)
                              catttATACTCGTCATACTTCAAGTTGCATGTACTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATg                                       >  1:1858431/4‑71 (MQ=11)
                               ccTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCact                                               <  1:5595546/62‑1 (MQ=40)
                               ccTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTAt                                        <  1:3426399/69‑1 (MQ=40)
                               caatataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGt                                      <  1:3273816/68‑1 (MQ=14)
                               caatataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGt                                      <  1:3267142/68‑1 (MQ=14)
                                tttATACTCGTCATACTTCAAGTTGCATGTACTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATGTa                                     <  1:5054026/70‑1 (MQ=11)
                                cTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTa                                     <  1:1814774/71‑1 (MQ=38)
                                cTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTa                                     <  1:750181/71‑1 (MQ=38)
                                cTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTa                                     <  1:5454205/71‑1 (MQ=38)
                                aatataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTa                                     <  1:2031418/69‑1 (MQ=14)
                                 ttATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCt                                                            <  1:486238/47‑1 (MQ=40)
                                 ttATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGt                                      >  1:2729835/1‑69 (MQ=37)
                                 ttATACTCGTCATACTTCAAGTTGCATGTACTGCGACTGCGTTCgtt                                                            >  1:2596227/1‑45 (MQ=11)
                                  tataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCt                                                            <  1:621709/46‑1 (MQ=38)
                                  tataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCt                                                            <  1:3633187/46‑1 (MQ=38)
                                   ataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCa                                                          >  1:4464717/1‑47 (MQ=38)
                                   ataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCa                                                          >  1:4838253/1‑47 (MQ=38)
                                   ataCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGc                                  >  1:257249/1‑71 (MQ=34)
                                    taCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCa                                                     <  1:5614210/51‑1 (MQ=33)
                                    taCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCa                                                     <  1:5612353/51‑1 (MQ=33)
                                    taCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCa                                                     <  1:4297026/51‑1 (MQ=33)
                                    taCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCa                                                     <  1:4488020/51‑1 (MQ=33)
                                    taCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCac                                                >  1:1633490/1‑56 (MQ=33)
                                    taCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAg                                   >  1:4595407/1‑69 (MQ=33)
                                    taCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAg                                   >  1:4974187/1‑69 (MQ=33)
                                    taCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGc                                  <  1:4705768/70‑1 (MQ=33)
                                    taCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGc                                  >  1:1607049/1‑70 (MQ=33)
                                    taCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGc                                  >  1:2895110/1‑70 (MQ=33)
                                    taCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGc                                  <  1:3516784/70‑1 (MQ=33)
                                    taCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCt                                 <  1:3829224/71‑1 (MQ=32)
                                     aCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTa                                     <  1:5261431/66‑1 (MQ=31)
                                     aCTCGGCATACTTCAAGTTGCATGTGCTGCGTCTGCGGTCGCTCACCCCAGTCACTTACTTATGTa                                     <  1:3291608/66‑1 (MQ=255)
                                                        gCATGTGCTTCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCtt              <  1:3689893/70‑1 (MQ=25)
                                                         cATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTg             <  1:4949375/70‑1 (MQ=35)
                                                         cATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGt            <  1:1387514/71‑1 (MQ=34)
                                                          aTGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTc           >  1:4301888/1‑71 (MQ=34)
                                                          aTGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTc           >  1:52988/1‑71 (MQ=34)
                                                           tgtgCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTc           >  1:37305/1‑70 (MQ=35)
                                                             tgctgcGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGcc        >  1:2083935/1‑71 (MQ=34)
                                                             tgctgcGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGc         >  1:2514053/1‑70 (MQ=35)
                                                               ctgcgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCtt      <  1:614027/71‑1 (MQ=34)
                                                               ctgcgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCtt      <  1:630184/71‑1 (MQ=34)
                                                                   gtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTg  >  1:5211613/1‑71 (MQ=34)
                                                                    |                                                                     
TTAGACATGTCTCTTGTCCTGTATCGTCGGGCCTTATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCTCTTGTCGCCTTCCTG  >  Exported/1960063‑1960200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: