| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,041,438 | G→A | 67.5% | D32N (GAT→AAT) | yqiI → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,041,438 | 0 | G | A | 67.5% | 89.9 / 67.4 | 83 | D32N (GAT→AAT) | yqiI | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (10/17); new base A (28/28); total (38/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01 | |||||||||||
TTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCT > Exported/2041373‑2041507 | tttttATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATAcc > 1:2141042/1‑71 (MQ=255)tttttATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAAt < 1:4047506/68‑1 (MQ=255)tttttATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATAcc > 1:1793979/1‑71 (MQ=255)tttttATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATAc > 1:2315947/1‑70 (MQ=255) tttATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCg < 1:3563194/70‑1 (MQ=255) tATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGtt < 1:3524237/70‑1 (MQ=255) tATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGtt < 1:1775562/70‑1 (MQ=255) gTTTTAGTTGATTAGCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCAtt < 1:1062939/71‑1 (MQ=255) gTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCAtt < 1:3521847/71‑1 (MQ=255) ttttAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTg > 1:4844340/1‑71 (MQ=255) ttAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGt > 1:4657081/1‑71 (MQ=255) ttAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGt > 1:5697283/1‑71 (MQ=255) tAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGt < 1:2891877/70‑1 (MQ=255) tAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGt < 1:2857530/70‑1 (MQ=255) tAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGt < 1:5423064/70‑1 (MQ=255) tAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGt < 1:1373246/70‑1 (MQ=255) ttCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCg < 1:2985009/56‑1 (MQ=255) ttCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCg < 1:3899831/56‑1 (MQ=255) ttCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGt < 1:1584279/67‑1 (MQ=255) ttCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACg < 1:277540/71‑1 (MQ=255) tCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGt < 1:5251869/66‑1 (MQ=255) tCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACg > 1:2151480/1‑70 (MQ=255) tCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACg > 1:1926305/1‑70 (MQ=255) cATTACCTGCATGGGCAATGGACTGCTATTCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGt < 1:881452/70‑1 (MQ=255) cATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGt > 1:3977535/1‑65 (MQ=255) cATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGt > 1:5031913/1‑65 (MQ=255) cATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGt > 1:5289081/1‑65 (MQ=255) cATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTa > 1:5625310/1‑71 (MQ=255) aTTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGt < 1:1294255/69‑1 (MQ=255) aCCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTAcc > 1:2132674/1‑69 (MQ=255) aCCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTAc > 1:3332393/1‑68 (MQ=255) ccTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAg < 1:1276411/71‑1 (MQ=255) ccTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAg < 1:1693992/71‑1 (MQ=255) ccTGCATGGGCAATGGACTGCTATGCTGAACATCAAGGTGGAAATACCGTTGTCATTGGTTACGTAcc > 1:4927517/1‑68 (MQ=255) cTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGa < 1:4356270/71‑1 (MQ=255) tGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGaa > 1:2532623/1‑71 (MQ=255) gCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCa > 1:4558904/1‑52 (MQ=255) cATGGGCAATGGACTGCTATGCTGCACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAAt < 1:4288348/70‑1 (MQ=255) cATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGt > 1:2008738/1‑56 (MQ=255) cATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTa > 1:5680257/1‑62 (MQ=255) cATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAAtc > 1:1402379/1‑71 (MQ=255) cATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAAt < 1:3027194/70‑1 (MQ=255) aTGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAAtct < 1:4328076/71‑1 (MQ=255) tGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCg > 1:2332459/1‑43 (MQ=255) gggCAATGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTa > 1:3046636/1‑59 (MQ=255) ggCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTa > 1:386393/1‑71 (MQ=255) aTGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACg > 1:2094443/1‑52 (MQ=255) aTGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACg > 1:3424723/1‑52 (MQ=255) aTGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACg > 1:3368375/1‑52 (MQ=255) tGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTAGGTACCAAGAATctct > 1:4346173/1‑65 (MQ=255) tGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACg < 1:2600859/51‑1 (MQ=255) tGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACg < 1:3910435/51‑1 (MQ=255) tGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACg < 1:4552632/51‑1 (MQ=255) tGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACg < 1:3335632/51‑1 (MQ=255) tGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATcccc < 1:2980024/71‑1 (MQ=255) tGGACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATccc > 1:4235889/1‑70 (MQ=255) ggACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAAtctc < 1:1809469/63‑1 (MQ=255) ggACTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATccc > 1:3896473/1‑69 (MQ=255) aCTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGc < 1:5557865/71‑1 (MQ=255) aCTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGc < 1:3692540/71‑1 (MQ=255) cTGCTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGc < 1:3920871/70‑1 (MQ=255) gCTATGCTGAACATGAAGGTGGAGATACCGTTGTCAt > 1:2204896/1‑37 (MQ=255) cTATGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATg > 1:3172588/1‑71 (MQ=255) tATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATg < 1:3706266/70‑1 (MQ=255) tGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTAc < 1:281847/44‑1 (MQ=255) tGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTa > 1:3923171/1‑43 (MQ=255) tGCTGAACATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCa > 1:2006852/1‑62 (MQ=255) cTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTaa < 1:2136776/70‑1 (MQ=255) tGAACATGAAGGTGGAGATACCGTTGTCAGTGGTTACGTACCAAGAAtct > 1:5163355/1‑50 (MQ=255) aaCATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTaaaa < 1:2969979/69‑1 (MQ=255) aaCATGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTaaaa < 1:1314020/69‑1 (MQ=255) aTGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTaaaaa < 1:1281944/67‑1 (MQ=255) tGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGaa > 1:1531711/1‑41 (MQ=255) tGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGc < 1:2922496/55‑1 (MQ=255) tGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCACCAGc < 1:4027550/55‑1 (MQ=255) tGAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATctct < 1:4099404/46‑1 (MQ=255) gAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGc < 1:4513307/54‑1 (MQ=255) gAAGGTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAg > 1:4064851/1‑67 (MQ=255) aaGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGa > 1:3931014/1‑55 (MQ=255) aaGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGa > 1:951085/1‑55 (MQ=255) aGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATa < 1:3735510/71‑1 (MQ=255) ggTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATaa < 1:3882761/71‑1 (MQ=255) gTGGAAATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATaaa < 1:709657/71‑1 (MQ=255) gagaTACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAAt < 1:4560122/70‑1 (MQ=255) agaTACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCt > 1:852025/1‑71 (MQ=255) | TTTTTATGGGTTTTAGTTCATTACCTGCATGGGCAATGGACTGCTATGCTGAACATGAAGGTGGAGATACCGTTGTCATTGGTTACGTACCAAGAATCTCTATCCCCAGCGATGGTAAAAAAGGTGATAAAATCT > Exported/2041373‑2041507 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |