Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,045,835 C→T 6.4% intergenic (+597/+198) yqiK → / ← rfaE conserved hypothetical protein/fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,045,8350CT 6.4% 293.5 / 7.6 109intergenic (+597/+198)yqiK/rfaEconserved hypothetical protein/fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase
Reads supporting (aligned to +/- strand):  ref base C (41/61);  new base T (3/4);  total (44/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

Only 100 of 114 total aligned reads displayed.

GTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGA  >  Exported/2045765‑2045905
                                                                       |                                                                      
       gcTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGta                                                                   >  1:5442407/1‑69 (MQ=255)
           atcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGtat                                                                  <  1:2333700/66‑1 (MQ=255)
           atcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                              >  1:3343442/1‑70 (MQ=255)
           atcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAg                                                               <  1:5466060/69‑1 (MQ=255)
            tcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTg                                                                     <  1:4827154/62‑1 (MQ=255)
            tcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTg                                                                     <  1:1552405/62‑1 (MQ=255)
            tcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGaa                                                            <  1:2717963/71‑1 (MQ=255)
              aacaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAg                                                          >  1:5640262/1‑71 (MQ=255)
               ctaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGaaa                                                           <  1:4132819/67‑1 (MQ=255)
               acaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTg                                                                     <  1:5090951/59‑1 (MQ=255)
               acaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTg                                                                     <  1:1271467/59‑1 (MQ=255)
               acaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTg                                                                     <  1:711585/59‑1 (MQ=255)
                         ggCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                              <  1:346786/56‑1 (MQ=255)
                         ggCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                              <  1:276982/56‑1 (MQ=255)
                         ggCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                              <  1:4051813/56‑1 (MQ=255)
                         ggCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                              <  1:2562837/56‑1 (MQ=255)
                          gcagcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                              <  1:165441/55‑1 (MQ=255)
                          gcagcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGa                                                             <  1:733399/56‑1 (MQ=255)
                           cagcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAg                                             >  1:830053/1‑71 (MQ=255)
                            agcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTa                                                     <  1:2531398/62‑1 (MQ=255)
                            agcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTa                                                     <  1:4329956/62‑1 (MQ=255)
                             gcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                              <  1:4661619/52‑1 (MQ=255)
                             gcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAgg                                                         <  1:3000704/57‑1 (MQ=255)
                             gcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTatg                                                   >  1:4328361/1‑63 (MQ=255)
                               aaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGt                                                       >  1:781119/1‑57 (MQ=255)
                                aGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTa                                                     >  1:3736880/1‑58 (MQ=255)
                                aGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTc                                          <  1:657273/69‑1 (MQ=255)
                                  gggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGTCCTTGTATAAGGAAAGGGTTa                                                     <  1:1977594/56‑1 (MQ=255)
                                  acgCTGAAACGGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGt                                        >  1:303292/3‑70 (MQ=0)
                                   ggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat                                     >  1:552372/1‑71 (MQ=255)
                                   ggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat                                     >  1:5627124/1‑71 (MQ=255)
                                   cgCTGAAACGGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGtca                                      <  1:1786500/70‑1 (MQ=1)
                                     cTGAAACGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGaaa                                                           >  1:3653992/1‑47 (MQ=14)
                                     cTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATaa                                                                >  1:442617/1‑42 (MQ=255)
                                       gAAACGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGaa                                                            >  1:1492151/1‑44 (MQ=14)
                                       gAAACGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACtcat                                     >  1:1889915/1‑67 (MQ=14)
                                       gAAACGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACtcat                                     >  1:1896669/1‑67 (MQ=14)
                                       gAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGtt                                                      <  1:1190765/50‑1 (MQ=255)
                                        aaaCGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCatgat                                                 <  1:4403377/54‑1 (MQ=14)
                                        aaaCGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcatcg                                <  1:565443/71‑3 (MQ=11)
                                        aaaCGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc                                >  1:4899675/1‑71 (MQ=255)
                                         aaCGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAg                                             >  1:3535738/1‑57 (MQ=255)
                                          aCGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCAtgatga                                               >  1:3633700/1‑54 (MQ=14)
                                          aCGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCAtgatga                                               >  1:3590871/1‑54 (MQ=14)
                                          aCGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAATGAAAGGGTTATGATGAAGCTCGt                                        <  1:5193989/61‑1 (MQ=255)
                                             ggAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCatgat                                                 <  1:2622584/49‑1 (MQ=14)
                                             ggAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTCATCATCGTGgtg                           >  1:1155474/1‑70 (MQ=2)
                                             ggAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgtt                           >  1:3793091/1‑71 (MQ=255)
                                              gAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgt                            >  1:3680797/1‑70 (MQ=11)
                                              gAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTCATCATCGTGgtgc                          >  1:3332284/1‑69 (MQ=2)
                                              gAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttg                          <  1:2081641/71‑1 (MQ=255)
                                               aaaGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTCATCATCGTGgtgc                          >  1:3132334/1‑68 (MQ=2)
                                               aaaGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTCATCATCGTGgtgc                          >  1:4164927/1‑68 (MQ=2)
                                                 aGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGt                                                       <  1:22867/40‑1 (MQ=0)
                                                    cccTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACt                               <  1:1253870/60‑1 (MQ=255)
                                                     tcTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgtg                           <  1:3123410/63‑2 (MQ=11)
                                                     ccTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttgt                    >  1:3907027/1‑70 (MQ=255)
                                                      cTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtc                                       <  1:1663603/50‑1 (MQ=255)
                                                      cTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtc                                       <  1:3373786/50‑1 (MQ=255)
                                                      cTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTa                  <  1:4919004/71‑1 (MQ=255)
                                                       tCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCat                                                    <  1:128161/36‑1 (MQ=12)
                                                       tCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCat                                                    <  1:1943990/36‑1 (MQ=12)
                                                        cccGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCtac                                         >  1:74665/1‑44 (MQ=9)
                                                        cccGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGc                                            >  1:3127908/1‑43 (MQ=255)
                                                        cccGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa                                 >  1:4898656/1‑54 (MQ=255)
                                                         ccGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgtga                          >  1:3161910/1‑59 (MQ=2)
                                                         ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCatga                                                  >  1:1660542/1‑36 (MQ=12)
                                                         ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCatga                                                  >  1:4550644/1‑36 (MQ=12)
                                                         ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat                                  <  1:213885/52‑1 (MQ=9)
                                                         ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat                                  <  1:3118384/52‑1 (MQ=9)
                                                         ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat                                  <  1:3479904/52‑1 (MQ=9)
                                                         ccGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttgt                    <  1:2337461/66‑1 (MQ=255)
                                                          cGAGGAAGGGGCCATAAATAAGGAAAGGGTCatgat                                                 <  1:3931266/36‑1 (MQ=9)
                                                          cGAGGAAGGGGCCATAAATAAGGAAAGGGTCatgat                                                 <  1:3598393/36‑1 (MQ=9)
                                                          cGAGGAAGGGGCCATAAATAAGGAAAGGGTCatgat                                                 <  1:2218638/36‑1 (MQ=9)
                                                          cGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa                                 >  1:1020149/1‑52 (MQ=255)
                                                          cGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg                              <  1:2294832/55‑1 (MQ=255)
                                                           gaagaaGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa                                 >  1:525181/1‑51 (MQ=255)
                                                           gaagaaGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt             <  1:4698006/71‑1 (MQ=255)
                                                           gaagaaGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt             <  1:4307788/71‑1 (MQ=255)
                                                           gaagaaGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGt              >  1:5507266/1‑70 (MQ=255)
                                                            aagaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgt                            >  1:1201610/1‑55 (MQ=255)
                                                            aagaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt             >  1:3440667/1‑70 (MQ=255)
                                                            aagaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTa            <  1:1849759/71‑1 (MQ=255)
                                                            aagaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTa            <  1:2702621/71‑1 (MQ=255)
                                                             agaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTgg                             >  1:611677/1‑53 (MQ=255)
                                                             agaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTgg                             >  1:3598080/1‑53 (MQ=255)
                                                             agaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa           <  1:1283014/71‑1 (MQ=255)
                                                             agaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa           <  1:4262499/71‑1 (MQ=255)
                                                              gaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAg          >  1:5332259/1‑71 (MQ=255)
                                                                 ggggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttg                     <  1:3400364/57‑1 (MQ=255)
                                                                   gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttg                     <  1:4397732/56‑1 (MQ=255)
                                                                   gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa           <  1:5556436/66‑1 (MQ=255)
                                                                   gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa           <  1:2402884/66‑1 (MQ=255)
                                                                     gCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTccc    <  1:2599328/71‑1 (MQ=255)
                                                                     gCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTccc    <  1:2279448/71‑1 (MQ=255)
                                                                      ccTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg                              <  1:5123806/44‑1 (MQ=255)
                                                                       cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa  >  1:5610479/1‑71 (MQ=255)
                                                                       cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa  >  1:2174414/1‑71 (MQ=255)
                                                                       cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa  >  1:4961894/1‑71 (MQ=255)
                                                                       |                                                                      
GTCCGGCGCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGA  >  Exported/2045765‑2045905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: