Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,045,838 G→A 27.8% intergenic (+600/+195) yqiK → / ← rfaE conserved hypothetical protein/fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,045,8380GA27.8% 198.6 / 68.3 108intergenic (+600/+195)yqiK/rfaEconserved hypothetical protein/fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase
Reads supporting (aligned to +/- strand):  ref base G (30/42);  new base A (14/15);  total (47/61)
Fisher's exact test for biased strand distribution p-value = 6.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 119 total aligned reads displayed.

GCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACT  >  Exported/2045772‑2045907
                                                                   |                                                                     
 cTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGtata                                                                   <  1:1887124/70‑1 (MQ=255)
    atcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGtat                                                                    <  1:2333700/66‑1 (MQ=255)
    atcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                                >  1:3343442/1‑70 (MQ=255)
    atcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAg                                                                 <  1:5466060/69‑1 (MQ=255)
     tcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTg                                                                       <  1:1552405/62‑1 (MQ=255)
     tcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTg                                                                       <  1:4827154/62‑1 (MQ=255)
     tcaACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGaa                                                              <  1:2717963/71‑1 (MQ=255)
       aacaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAg                                                            >  1:2466718/1‑71 (MQ=255)
       aacaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAg                                                            >  1:5640262/1‑71 (MQ=255)
        ctaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGaaa                                                             <  1:4132819/67‑1 (MQ=255)
        acaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTg                                                                       <  1:1271467/59‑1 (MQ=255)
        acaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTg                                                                       <  1:711585/59‑1 (MQ=255)
        acaaTCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTg                                                                       <  1:5090951/59‑1 (MQ=255)
                  ggCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                                <  1:276982/56‑1 (MQ=255)
                  ggCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                                <  1:2562837/56‑1 (MQ=255)
                  ggCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                                <  1:4051813/56‑1 (MQ=255)
                  ggCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                                <  1:346786/56‑1 (MQ=255)
                   gcagcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                                <  1:165441/55‑1 (MQ=255)
                   gcagcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGa                                                               <  1:733399/56‑1 (MQ=255)
                    cagcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAg                                               >  1:830053/1‑71 (MQ=255)
                     agcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTa                                                       <  1:4329956/62‑1 (MQ=255)
                     agcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTa                                                       <  1:2531398/62‑1 (MQ=255)
                      gcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAgg                                                                <  1:4661619/52‑1 (MQ=255)
                      gcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAgg                                                           <  1:3000704/57‑1 (MQ=255)
                      gcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTatg                                                     >  1:4328361/1‑63 (MQ=255)
                        aaGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGt                                                         >  1:781119/1‑57 (MQ=255)
                           gggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGTCCTTGTATAAGGAAAGGGTTa                                                       <  1:1977594/56‑1 (MQ=255)
                           acgCTGAAACGGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGt                                          >  1:303292/3‑70 (MQ=0)
                            ggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGtat                                                                    <  1:57129/42‑1 (MQ=255)
                            ggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat                                       >  1:5627124/1‑71 (MQ=255)
                            ggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat                                       >  1:3187602/1‑71 (MQ=255)
                            ggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat                                       >  1:552372/1‑71 (MQ=255)
                            cgCTGAAACGGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGtca                                        <  1:1786500/70‑1 (MQ=1)
                              cTGAAACGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGaaa                                                             >  1:3653992/1‑47 (MQ=14)
                              cTGAAACGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCatg                                                     <  1:144998/55‑1 (MQ=14)
                              cTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATaa                                                                  >  1:442617/1‑42 (MQ=255)
                                gAAACGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGaa                                                              >  1:1492151/1‑44 (MQ=14)
                                gAAACGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACtcat                                       >  1:1896669/1‑67 (MQ=14)
                                gAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGtt                                                        <  1:1190765/50‑1 (MQ=255)
                                 aaaCGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCatgat                                                   <  1:4403377/54‑1 (MQ=14)
                                 aaaCGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCatgat                                                   <  1:3787364/54‑1 (MQ=14)
                                 aaaCGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcatcg                                  <  1:565443/71‑3 (MQ=11)
                                 aaaCGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc                                  >  1:4899675/1‑71 (MQ=255)
                                  aaCGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAg                                               >  1:3535738/1‑57 (MQ=255)
                                   aCGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCAtgatga                                                 >  1:3633700/1‑54 (MQ=14)
                                   aCGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAATGAAAGGGTTATGATGAAGCTCGt                                          <  1:5193989/61‑1 (MQ=255)
                                      ggAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCatgat                                                   <  1:2622584/49‑1 (MQ=14)
                                      ggAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTCATCATCGTGgtg                             >  1:1155474/1‑70 (MQ=2)
                                      ggAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgtt                             >  1:3793091/1‑71 (MQ=255)
                                       gAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgt                              >  1:3680797/1‑70 (MQ=11)
                                       gAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTCATCATCGTGgtgc                            >  1:3332284/1‑69 (MQ=2)
                                       gAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttg                            <  1:2081641/71‑1 (MQ=255)
                                        aaaGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTCATCATCGTGgtgc                            >  1:3132334/1‑68 (MQ=2)
                                          aGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGt                                                         <  1:22867/40‑1 (MQ=0)
                                          aGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGt                                                         <  1:3505689/40‑1 (MQ=0)
                                             cccTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACt                                 <  1:1253870/60‑1 (MQ=255)
                                              tcTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgtg                             <  1:3123410/63‑2 (MQ=11)
                                              ccTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttgt                      >  1:3907027/1‑70 (MQ=255)
                                               cTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtc                                         <  1:3373786/50‑1 (MQ=255)
                                               cTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtc                                         <  1:1663603/50‑1 (MQ=255)
                                               cTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTa                    <  1:4919004/71‑1 (MQ=255)
                                                tCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCat                                                      <  1:2965762/36‑1 (MQ=12)
                                                tCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCat                                                      <  1:1943990/36‑1 (MQ=12)
                                                 cccGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCtac                                           >  1:74665/1‑44 (MQ=9)
                                                 cccGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGc                                              >  1:3127908/1‑43 (MQ=255)
                                                 cccGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa                                   >  1:4898656/1‑54 (MQ=255)
                                                  ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCatga                                                    >  1:5472944/1‑36 (MQ=12)
                                                  ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCatga                                                    >  1:4550644/1‑36 (MQ=12)
                                                  ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat                                    <  1:3118384/52‑1 (MQ=9)
                                                  ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat                                    <  1:213885/52‑1 (MQ=9)
                                                  ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat                                    <  1:3479904/52‑1 (MQ=9)
                                                   cGAGGAAGGGGCCATAAATAAGGAAAGGGTCatgat                                                   <  1:3931266/36‑1 (MQ=9)
                                                   cGAGGAAGGGGCCATAAATAAGGAAAGGGTCatgat                                                   <  1:3598393/36‑1 (MQ=9)
                                                   cGAGGAAGGGGCCATAAATAAGGAAAGGGTCatgat                                                   <  1:2218638/36‑1 (MQ=9)
                                                   cGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa                                   >  1:1020149/1‑52 (MQ=255)
                                                   cGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg                                <  1:2294832/55‑1 (MQ=255)
                                                    gaagaaGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa                                   >  1:525181/1‑51 (MQ=255)
                                                    gaagaaGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt               <  1:4698006/71‑1 (MQ=255)
                                                    gaagaaGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt               <  1:4307788/71‑1 (MQ=255)
                                                    gaagaaGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGt                >  1:5507266/1‑70 (MQ=255)
                                                     aagaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgt                              >  1:1201610/1‑55 (MQ=255)
                                                     aagaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTa              <  1:1849759/71‑1 (MQ=255)
                                                     aagaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTa              <  1:2702621/71‑1 (MQ=255)
                                                      agaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTgg                               >  1:611677/1‑53 (MQ=255)
                                                      agaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTgg                               >  1:4303137/1‑53 (MQ=255)
                                                      agaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTgg                               >  1:3598080/1‑53 (MQ=255)
                                                      agaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa             <  1:4262499/71‑1 (MQ=255)
                                                      agaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa             <  1:1283014/71‑1 (MQ=255)
                                                       gaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAg            >  1:5332259/1‑71 (MQ=255)
                                                          ggggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttg                       <  1:3400364/57‑1 (MQ=255)
                                                          ggggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttg                       <  1:5080620/57‑1 (MQ=255)
                                                            gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa             <  1:5556436/66‑1 (MQ=255)
                                                            gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa             <  1:2402884/66‑1 (MQ=255)
                                                              gCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTccc      <  1:2279448/71‑1 (MQ=255)
                                                               ccTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg                                <  1:5123806/44‑1 (MQ=255)
                                                                cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa    >  1:2174414/1‑71 (MQ=255)
                                                                cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa    >  1:4961894/1‑71 (MQ=255)
                                                                   gTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTa                    >  1:3449558/1‑52 (MQ=255)
                                                                   gTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACt                  >  1:2513298/1‑54 (MQ=255)
                                                                   gTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACt  >  1:1802981/1‑70 (MQ=255)
                                                                   |                                                                     
GCTCATCAACAATCGGGGGGCAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACT  >  Exported/2045772‑2045907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: