Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,045,864 T→A 10.6% intergenic (+626/+169) yqiK → / ← rfaE conserved hypothetical protein/fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,045,8640TA10.6% 312.8 / 26.8 125intergenic (+626/+169)yqiK/rfaEconserved hypothetical protein/fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase
Reads supporting (aligned to +/- strand):  ref base T (57/54);  new base A (4/9);  total (61/64)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

Only 100 of 126 total aligned reads displayed.

AGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAG  >  Exported/2045797‑2045933
                                                                    |                                                                     
  acgCTGAAACGGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGt                                                                    >  1:303292/3‑70 (MQ=0)
   ggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat                                                                 >  1:3187602/1‑71 (MQ=255)
   ggCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat                                                                 >  1:552372/1‑71 (MQ=255)
       gAAACGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACtcat                                                                 >  1:1889915/1‑67 (MQ=14)
        aaaCGGGAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcatcg                                                            <  1:565443/71‑3 (MQ=11)
        aaaCGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc                                                            >  1:4899675/1‑71 (MQ=255)
             ggAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTCATCATCGTGgtg                                                       >  1:1155474/1‑70 (MQ=2)
             ggAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgtt                                                       >  1:3793091/1‑71 (MQ=255)
              gAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgt                                                        >  1:3680797/1‑70 (MQ=11)
              gAAAGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTCATCATCGTGgtgc                                                      >  1:3332284/1‑69 (MQ=2)
              gAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttg                                                      <  1:2081641/71‑1 (MQ=255)
               aaaGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTCATCATCGTGgtgc                                                      >  1:4164927/1‑68 (MQ=2)
               aaaGCCCCTCCCGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTCATCATCGTGgtgc                                                      >  1:3132334/1‑68 (MQ=2)
                    cccTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACt                                                           <  1:1253870/60‑1 (MQ=255)
                     tcTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgtg                                                       <  1:3123410/63‑2 (MQ=11)
                     ccTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttgt                                                >  1:3907027/1‑70 (MQ=255)
                      cTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtc                                                                   <  1:3373786/50‑1 (MQ=255)
                      cTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtc                                                                   <  1:1663603/50‑1 (MQ=255)
                      cTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTa                                              <  1:4919004/71‑1 (MQ=255)
                        cccGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCtac                                                                     >  1:74665/1‑44 (MQ=9)
                         ccGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgtga                                                      >  1:3161910/1‑59 (MQ=2)
                         ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat                                                              <  1:3118384/52‑1 (MQ=9)
                         ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat                                                              <  1:3479904/52‑1 (MQ=9)
                         ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat                                                              <  1:2184869/52‑1 (MQ=9)
                         ccGAGGAAGGGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat                                                              <  1:213885/52‑1 (MQ=9)
                         ccGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttgt                                                <  1:2337461/66‑1 (MQ=255)
                          cGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa                                                             >  1:1020149/1‑52 (MQ=255)
                          cGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg                                                          <  1:2294832/55‑1 (MQ=255)
                           gaagaaGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa                                                             >  1:525181/1‑51 (MQ=255)
                           gaagaaGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt                                         <  1:4307788/71‑1 (MQ=255)
                           gaagaaGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt                                         <  1:4698006/71‑1 (MQ=255)
                            aagaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTa                                        <  1:1849759/71‑1 (MQ=255)
                            aagaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTa                                        <  1:2702621/71‑1 (MQ=255)
                             agaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTgg                                                         >  1:3598080/1‑53 (MQ=255)
                             agaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTgg                                                         >  1:611677/1‑53 (MQ=255)
                             agaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTgg                                                         >  1:4303137/1‑53 (MQ=255)
                             agaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa                                       <  1:1283014/71‑1 (MQ=255)
                              gaagGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAg                                      >  1:5332259/1‑71 (MQ=255)
                                 ggggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttg                                                 <  1:3400364/57‑1 (MQ=255)
                                 ggggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttg                                                 <  1:5080620/57‑1 (MQ=255)
                                   gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttg                                                 <  1:4397732/56‑1 (MQ=255)
                                   gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa                                       <  1:5556436/66‑1 (MQ=255)
                                   gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa                                       <  1:2402884/66‑1 (MQ=255)
                                     gCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTccc                                <  1:2599328/71‑1 (MQ=255)
                                     gCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTccc                                <  1:2279448/71‑1 (MQ=255)
                                      ccTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg                                                          <  1:5123806/44‑1 (MQ=255)
                                       cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa                              >  1:4961894/1‑71 (MQ=255)
                                       cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa                              >  1:2174414/1‑71 (MQ=255)
                                       cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa                              >  1:5610479/1‑71 (MQ=255)
                                          gTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTa                                              >  1:3449558/1‑52 (MQ=255)
                                          gTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACt                            <  1:2989076/70‑1 (MQ=255)
                                          gTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACt                            >  1:5045282/1‑70 (MQ=255)
                                          gTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACt                            >  1:1802981/1‑70 (MQ=255)
                                          gTATAAGGAAAGGGTTATGATGAAGCTCGTCAACATACTg                                                          >  1:4741596/1‑40 (MQ=255)
                                           tataAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGttt                                   <  1:2037146/62‑1 (MQ=255)
                                           tataAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACtt                           <  1:2360843/70‑1 (MQ=255)
                                           tataAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACtt                           <  1:4805201/70‑1 (MQ=255)
                                           aataAGGAAAGGGTTATGATGAAGCACGTCATCATACTgg                                                         >  1:1658768/2‑40 (MQ=14)
                                            ataAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTAc                         >  1:3781916/1‑71 (MQ=255)
                                            ataAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgt                                                        <  1:1232592/40‑1 (MQ=255)
                                            ataAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgt                                                        <  1:177545/40‑1 (MQ=255)
                                             taAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTAc                         >  1:3135905/1‑70 (MQ=255)
                                             taAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACt                        <  1:4184059/71‑1 (MQ=255)
                                             taAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgtga                                                      <  1:5329727/41‑3 (MQ=14)
                                             taAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgtga                                                      <  1:2103245/41‑3 (MQ=14)
                                             taAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgtga                                                      <  1:1334239/41‑3 (MQ=14)
                                              aaGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACt                        >  1:1233939/1‑70 (MQ=255)
                                               aGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa                                       >  1:486568/1‑54 (MQ=255)
                                               aGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa                                       >  1:385031/1‑54 (MQ=255)
                                                  aaaGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTaacaa                   >  1:1408650/1‑71 (MQ=255)
                                                  aaaGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTaacaa                   <  1:1078939/71‑1 (MQ=255)
                                                   aaGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTGCTGTTAAGTTTCCCGACTTACTaacaa                   >  1:777201/1‑70 (MQ=255)
                                                   aaGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGttt                                   >  1:1940289/1‑54 (MQ=255)
                                                   aaGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGtt                                    >  1:5557866/1‑53 (MQ=255)
                                                    aGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACt                 <  1:1435832/71‑1 (MQ=255)
                                                    aGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACt                 <  1:4297176/71‑1 (MQ=255)
                                                     gggTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa                              >  1:4734498/1‑57 (MQ=255)
                                                       gTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACtcat              <  1:2724373/71‑1 (MQ=255)
                                                       gTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACtcat              <  1:2787557/71‑1 (MQ=255)
                                                       gTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACtcat              <  1:1948681/71‑1 (MQ=255)
                                                         tATGATGACGAGCTTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACtcat              <  1:4135275/69‑1 (MQ=25)
                                                           tgatgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt                                         <  1:4025806/40‑1 (MQ=255)
                                                           tgatgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt                                         <  1:3335732/40‑1 (MQ=255)
                                                            gatgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTaac                     <  1:84775/59‑1 (MQ=255)
                                                             atgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa                                       >  1:135141/1‑40 (MQ=255)
                                                             atgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTa                          >  1:1475997/1‑53 (MQ=255)
                                                              tgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTa                                        <  1:5675658/38‑1 (MQ=255)
                                                              tgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTa                                        <  1:640693/38‑1 (MQ=255)
                                                              tgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAg                                      >  1:1020266/1‑40 (MQ=255)
                                                              tgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTc                                  <  1:283267/44‑1 (MQ=255)
                                                              tgaAGCACGTCATCATACTGGTGATACTCTTAGTGATTAGCTTCCAGGCTTACTaagaac                  <  1:526827/60‑1 (MQ=255)
                                                                aaGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAg                                      >  1:3223184/1‑38 (MQ=255)
                                                                aaGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAggggg     <  1:3028699/71‑1 (MQ=255)
                                                                 aGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAggggg     >  1:483794/1‑70 (MQ=255)
                                                                  gCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa                                       >  1:4986244/1‑35 (MQ=255)
                                                                  gCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAg                                      >  1:181965/1‑36 (MQ=255)
                                                                  gCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGga   <  1:4943335/71‑1 (MQ=255)
                                                                  gCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGga   <  1:4607599/71‑1 (MQ=255)
                                                                   cTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGgag  <  1:1910649/71‑1 (MQ=255)
                                                                   cTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGgag  <  1:1903942/71‑1 (MQ=255)
                                                                    |                                                                     
AGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAGGGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAG  >  Exported/2045797‑2045933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: