Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 2,053,941 | G→A | 9.6% | intergenic (+99/+82) | cca → / ← bacA | fused tRNA nucleotidyl transferase and 2'3'‑cyclic phosphodiesterase/2'nucleotidase/phosphatase/undecaprenyl pyrophosphate phosphatase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 2,053,941 | 0 | G | A | 9.6% | 143.6 / 4.8 | 52 | intergenic (+99/+82) | cca/bacA | fused tRNA nucleotidyl transferase and 2'3'‑cyclic phosphodiesterase/2'nucleotidase/phosphatase/undecaprenyl pyrophosphate phosphatase |
Reads supporting (aligned to +/- strand): ref base G (28/19); new base A (3/2); total (31/21) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01 |
AACGCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCC > Exported/2053872‑2054007 | aaCGCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGt > 1:1842913/1‑71 (MQ=255) aCGCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGGAAGCGTCGCATCAGGCGtt > 1:3875910/1‑71 (MQ=255) aCGCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGtt < 1:129242/71‑1 (MQ=255) cGCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGtt > 1:3281464/1‑70 (MQ=255) gCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGa > 1:328791/1‑71 (MQ=255) ttATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGtt < 1:1417792/66‑1 (MQ=255) ttATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTg > 1:5198770/1‑71 (MQ=255) tATCCGGCCTACAGGTCGGCAACAGTTGTAGGCCTGATAAGACGCGACAGCGTCGCATCAGGCattgattg > 1:4402974/1‑71 (MQ=2) aTCCGGCCTACAGGTCGGCAACAGTTGTAGGCCTGATAAGACGCGACAGCGTCGCATCAGGCATTGATTGc > 1:3497838/1‑71 (MQ=2) aTCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTg < 1:1559422/69‑1 (MQ=255) ggccTACAGGTCGGCAACAGTTGTAGGCCTGATAAGACGCGACAGCGTCGCATCAGGCATTGATTGCCGGa > 1:5129818/4‑71 (MQ=14) gg‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGAt > 1:2698069/1‑71 (MQ=255) aTGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGAtt < 1:3024428/58‑1 (MQ=255) aTGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATgcgg < 1:1681334/69‑1 (MQ=255) tGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATgcggc < 1:1625650/69‑1 (MQ=255) gTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGt > 1:4160627/1‑70 (MQ=255) cAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGaa > 1:4671056/1‑71 (MQ=255) aaCGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAAc > 1:2545792/1‑71 (MQ=255) cGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGc < 1:483099/71‑1 (MQ=255) cGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGc > 1:688297/1‑71 (MQ=255) gtttGTAGGCCTGATAAGACGCGACAGCGTCGCATCAGGCATTGATTGCCGGATGCGGCGTAAACGCCtt < 1:4293478/68‑1 (MQ=11) gtttGTAGGCCTGATAAGACGCGACAGCGTCGCATCAGGCATTGATTGCCGGATGCGGCGTAAACGCCtt < 1:3890482/68‑1 (MQ=11) aGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTa < 1:1025895/71‑1 (MQ=255) gTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCg < 1:4145213/50‑1 (MQ=255) gTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTAt < 1:5202251/71‑1 (MQ=255) ttGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGCAt < 1:4536747/52‑1 (MQ=255) tGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAAc > 1:2172949/1‑62 (MQ=255) gTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGGTTGCCGAATGCGGCGTAAACGCCTTATCCg > 1:11775/1‑71 (MQ=14) gTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGGTTGCCGAATGCGGCGTAAACGCCTTATCCg > 1:384209/1‑71 (MQ=14) ggCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGcc < 1:3706205/71‑1 (MQ=255) cTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTAc > 1:4226647/1‑71 (MQ=255) tGATAAGACGCGCAAGCGTCGCATCAGGCGTTGGTTGCCGAATGCGGCGta < 1:4217731/51‑2 (MQ=14) gATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACg > 1:5380770/1‑70 (MQ=255) aTAAGACGCGCAAGCGTCGCATCAGGCGTTGGTTGCCGAATGAGGCGTAAACGCCTTAt > 1:243636/1‑59 (MQ=17) aTAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGc > 1:4815312/1‑70 (MQ=255) aaGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAAt > 1:1385256/1‑71 (MQ=255) aGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATc < 1:5636696/71‑1 (MQ=255) gACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCg > 1:5637974/1‑71 (MQ=255) aCGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATgcggc < 1:1660086/41‑1 (MQ=255) aCGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGt > 1:3671525/1‑71 (MQ=255) cgcgCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGtt > 1:3079450/1‑71 (MQ=255) gcAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGTTGa > 1:946860/1‑70 (MQ=255) gcAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGTTGa > 1:1416237/1‑70 (MQ=255) gcAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGTTGAc < 1:4357513/71‑1 (MQ=255) gcAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGTTGAc < 1:5568931/71‑1 (MQ=255) acaGCGTCGCATCAGGCATTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc > 1:3716432/3‑58 (MQ=11) gCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAAc > 1:1767830/1‑39 (MQ=255) gCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCATATTCCGCCTACGCAATCGTTGACAACg > 1:2247135/1‑71 (MQ=255) gCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGTTGACAACGcc > 1:2436290/1‑68 (MQ=255) gCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGTTGACAACGCCaa < 1:394944/70‑1 (MQ=255) gCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAg > 1:4752680/1‑71 (MQ=255) aGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTa > 1:1099773/1‑44 (MQ=255) ggCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATcc < 1:1006173/70‑1 (MQ=255) | AACGCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGTTGACAACGCCAAGCATCC > Exported/2053872‑2054007 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |