Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,068,639 T→C 14.5% K384R (AAA→AGA) ‡ aer ← fused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,068,6390TC14.5% 160.2 / 17.2 69K384R (AAA→AGA) ‡aerfused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component
Reads supporting (aligned to +/- strand):  ref base T (30/29);  new base C (6/4);  total (36/33)
Fisher's exact test for biased strand distribution p-value = 7.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCA  >  Exported/2068569‑2068707
                                                                      |                                                                    
aaTATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCtt                                                                      <  1:5128433/71‑1 (MQ=255)
 atatCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCttt                                                                     <  1:3440332/71‑1 (MQ=255)
 atatCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCttt                                                                     >  1:642705/1‑71 (MQ=255)
  tatCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTg                                                                    <  1:3794506/71‑1 (MQ=255)
  tagcGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCttt                                                                     <  1:1950387/67‑1 (MQ=255)
    tCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCttt                                                                     >  1:1496479/1‑68 (MQ=255)
    tCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCttt                                                                     >  1:762290/1‑68 (MQ=255)
    tCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCttt                                                                     >  1:2017912/1‑68 (MQ=255)
    tCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGcc                                                                  >  1:1731936/1‑71 (MQ=255)
    tCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGcc                                                                  <  1:5241137/71‑1 (MQ=255)
       ttGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTg                                                               <  1:509632/71‑1 (MQ=255)
       ttGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTg                                                               <  1:2189034/71‑1 (MQ=255)
         ggCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGtt                                                             >  1:1260742/1‑71 (MQ=255)
         ggCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGtt                                                             >  1:2271021/1‑71 (MQ=255)
               aTTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCgg                                                       <  1:1934680/71‑1 (MQ=255)
                ttAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGcc                                                         <  1:2562609/68‑1 (MQ=255)
                ttAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCgg                                                       <  1:4988295/70‑1 (MQ=255)
                 tAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCa                                                     >  1:4627060/1‑71 (MQ=255)
                  aGCGCTGCGGCTGGCTAAATGCCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCg                                                        >  1:3394627/1‑67 (MQ=255)
                  aGCGCTGCGGCTGGCTAAATGCCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCAc                                                    >  1:1497492/1‑71 (MQ=255)
                  aGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCg                                                        >  1:288336/1‑67 (MQ=255)
                    cgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCAcgc                                                  <  1:2795825/71‑1 (MQ=255)
                     gcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCAcgcg                                                 <  1:3653829/71‑1 (MQ=255)
                      cTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACgcgc                                                <  1:31476/71‑1 (MQ=255)
                       tacGGCTGGCAAGATTGCGCACTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCgagc                                                >  1:949004/3‑70 (MQ=255)
                         cGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGc                                                                   >  1:4130272/1‑49 (MQ=255)
                         cGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGc                                                          >  1:2561294/1‑58 (MQ=255)
                            ctggctAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCGTc                                          >  1:5514198/1‑71 (MQ=255)
                                ctAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc                                                            <  1:5691084/49‑1 (MQ=255)
                                 tAAATGACGCACTTCCCCTGCCACCACTGCAAAACCtt                                                                      >  1:4270326/1‑38 (MQ=255)
                                 tAAATGACGCACTTCCCCTGCCACCACTGCAAAACCtt                                                                      >  1:289426/1‑38 (MQ=255)
                                 tAAATGACGCACTTCCCCTGCCACCACTGCAAAACCtt                                                                      >  1:45832/1‑38 (MQ=255)
                                  aaaTGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc                                                            <  1:96151/47‑1 (MQ=255)
                                    aTGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGt                                                              >  1:591358/1‑43 (MQ=255)
                                    aTGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGc                                                          >  1:3498637/1‑47 (MQ=255)
                                     tGACGCACTTCCCCTGCCACCACTGCAAAACCTTTg                                                                    <  1:4226627/36‑1 (MQ=255)
                                     tGACGCACTTCCCCTGCCACCACTGCAAAACCTTTg                                                                    <  1:1648839/36‑1 (MQ=255)
                                     tGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAccgccg                                  >  1:2579055/1‑70 (MQ=255)
                                     tGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAccgcc                                   >  1:4293917/1‑69 (MQ=255)
                                       aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGc                                                                   >  1:2773435/1‑35 (MQ=255)
                                       aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGcgcc                                               <  1:5137532/55‑1 (MQ=255)
                                       aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGcgcc                                               <  1:3223092/55‑1 (MQ=255)
                                       aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGcgcc                                               <  1:3152915/55‑1 (MQ=255)
                                        cGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt                              <  1:4839485/71‑1 (MQ=255)
                                             ttCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAggg                         >  1:4406751/1‑71 (MQ=255)
                                              tcaccTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCa                            >  1:3808880/4‑67 (MQ=255)
                                              tcaccTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGc                        >  1:1607488/4‑71 (MQ=255)
                                              tCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCa                                        >  1:5501552/1‑55 (MQ=255)
                                              tCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCa                                        >  1:3851941/1‑55 (MQ=255)
                                               ccccTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGc                        >  1:3133454/1‑70 (MQ=255)
                                                 ccTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAg                     <  1:3115023/71‑1 (MQ=255)
                                                   tGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt                              <  1:1804907/60‑1 (MQ=255)
                                                   tGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa                   >  1:4876798/1‑71 (MQ=255)
                                                    gCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGaa                   >  1:551201/1‑70 (MQ=255)
                                                    gCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa                   >  1:3128799/1‑70 (MQ=255)
                                                     ccactaCCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGaa                   <  1:1596229/69‑1 (MQ=255)
                                                     ccaccaCTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAc                                       >  1:1004843/1‑49 (MQ=255)
                                                      caccacTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat                <  1:3381062/71‑1 (MQ=255)
                                                      caccacAGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat                >  1:1366067/1‑71 (MQ=255)
                                                        ccacTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTg              <  1:3391711/71‑1 (MQ=255)
                                                        ccacCGCAAAACCGCGCCCTTGCTCACCCGCACGCGCAGCCTCAACCGCCGCGTTCAAAGCCAGAATATTg              >  1:2145271/1‑71 (MQ=255)
                                                        ccacCGCAAAACCGCGCCCTTGCTCACCCGCACGCGCAGCCTCAACCGCCGCGTTCAAAGCCAGAATATTg              <  1:5665247/71‑1 (MQ=255)
                                                          acTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCa                            >  1:872275/1‑55 (MQ=255)
                                                          acTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGt            >  1:1418717/1‑71 (MQ=255)
                                                           cTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTc                                          <  1:983823/40‑1 (MQ=255)
                                                           cTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTc           >  1:4854925/1‑71 (MQ=255)
                                                              cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAg                           >  1:560931/1‑52 (MQ=255)
                                                              cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTg         <  1:3944400/70‑1 (MQ=255)
                                                               aaaaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAata                 >  1:915605/1‑61 (MQ=255)
                                                               aaaaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAata                 >  1:5113625/1‑61 (MQ=255)
                                                                 aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTc                             <  1:4460276/47‑1 (MQ=255)
                                                                 aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc     <  1:310011/71‑1 (MQ=255)
                                                                 aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc     <  1:3913188/71‑1 (MQ=255)
                                                                 aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc     <  1:210974/71‑1 (MQ=255)
                                                                 aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc     >  1:2298716/1‑71 (MQ=255)
                                                                   ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACg    >  1:2514079/1‑70 (MQ=255)
                                                                   ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGc   <  1:61465/71‑1 (MQ=255)
                                                                   ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGc   <  1:1503705/71‑1 (MQ=255)
                                                                    cTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCa  <  1:3548251/71‑1 (MQ=255)
                                                                      |                                                                    
AATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCA  >  Exported/2068569‑2068707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: