Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,068,641 G→C 15.1% G383G (GGC→GGG aer ← fused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,068,6410GC15.1% 159.0 / 18.1 66G383G (GGC→GGGaerfused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component
Reads supporting (aligned to +/- strand):  ref base G (30/26);  new base C (6/4);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 7.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCA  >  Exported/2068571‑2068707
                                                                      |                                                                  
tatCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTg                                                                    <  1:3794506/71‑1 (MQ=255)
  tCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGcc                                                                  <  1:5241137/71‑1 (MQ=255)
  tCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGcc                                                                  >  1:1731936/1‑71 (MQ=255)
     ttGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTg                                                               <  1:2189034/71‑1 (MQ=255)
     ttGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTg                                                               <  1:509632/71‑1 (MQ=255)
       ggCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGtt                                                             >  1:1260742/1‑71 (MQ=255)
       ggCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGtt                                                             >  1:2271021/1‑71 (MQ=255)
             aTTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCgg                                                       <  1:1934680/71‑1 (MQ=255)
              ttAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGcc                                                         <  1:2562609/68‑1 (MQ=255)
              ttAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCgg                                                       <  1:4988295/70‑1 (MQ=255)
               tAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCa                                                     >  1:4627060/1‑71 (MQ=255)
                aGCGCTGCGGCTGGCTAAATGCCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCg                                                        >  1:3394627/1‑67 (MQ=255)
                aGCGCTGCGGCTGGCTAAATGCCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCAc                                                    >  1:1497492/1‑71 (MQ=255)
                aGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCg                                                        >  1:288336/1‑67 (MQ=255)
                  cgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCAcgc                                                  <  1:2795825/71‑1 (MQ=255)
                   gcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCAcgcg                                                 <  1:3653829/71‑1 (MQ=255)
                    cTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACgcgc                                                <  1:31476/71‑1 (MQ=255)
                     tacGGCTGGCAAGATTGCGCACTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCgagc                                                >  1:949004/3‑70 (MQ=255)
                       cGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGc                                                                   >  1:4130272/1‑49 (MQ=255)
                       cGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGc                                                          >  1:2561294/1‑58 (MQ=255)
                          ctggctAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCGTc                                          >  1:5514198/1‑71 (MQ=255)
                              ctAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc                                                            <  1:5691084/49‑1 (MQ=255)
                                aaaTGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc                                                            <  1:96151/47‑1 (MQ=255)
                                  aTGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGt                                                              >  1:591358/1‑43 (MQ=255)
                                  aTGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGc                                                          >  1:3498637/1‑47 (MQ=255)
                                   tGACGCACTTCCCCTGCCACCACTGCAAAACCTTTg                                                                    <  1:4226627/36‑1 (MQ=255)
                                   tGACGCACTTCCCCTGCCACCACTGCAAAACCTTTg                                                                    <  1:1648839/36‑1 (MQ=255)
                                   tGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAccgccg                                  >  1:2579055/1‑70 (MQ=255)
                                   tGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAccgcc                                   >  1:4293917/1‑69 (MQ=255)
                                     aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGc                                                                   >  1:2773435/1‑35 (MQ=255)
                                     aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGcgcc                                               <  1:3152915/55‑1 (MQ=255)
                                     aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGcgcc                                               <  1:3223092/55‑1 (MQ=255)
                                     aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGcgcc                                               <  1:5137532/55‑1 (MQ=255)
                                      cGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt                              <  1:4839485/71‑1 (MQ=255)
                                           ttCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAggg                         >  1:4406751/1‑71 (MQ=255)
                                            tcaccTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCa                            >  1:3808880/4‑67 (MQ=255)
                                            tcaccTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGc                        >  1:1607488/4‑71 (MQ=255)
                                            tCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCa                                        >  1:3851941/1‑55 (MQ=255)
                                            tCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCa                                        >  1:5501552/1‑55 (MQ=255)
                                             ccccTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGc                        >  1:3133454/1‑70 (MQ=255)
                                               ccTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAg                     <  1:3115023/71‑1 (MQ=255)
                                                 tGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt                              <  1:1804907/60‑1 (MQ=255)
                                                 tGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa                   >  1:4876798/1‑71 (MQ=255)
                                                  gCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGaa                   >  1:551201/1‑70 (MQ=255)
                                                  gCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa                   >  1:3128799/1‑70 (MQ=255)
                                                   ccactaCCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGaa                   <  1:1596229/69‑1 (MQ=255)
                                                   ccaccaCTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAc                                       >  1:1004843/1‑49 (MQ=255)
                                                    caccacTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat                <  1:3381062/71‑1 (MQ=255)
                                                    caccacAGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat                >  1:1366067/1‑71 (MQ=255)
                                                      ccacTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTg              <  1:3391711/71‑1 (MQ=255)
                                                      ccacCGCAAAACCGCGCCCTTGCTCACCCGCACGCGCAGCCTCAACCGCCGCGTTCAAAGCCAGAATATTg              <  1:5665247/71‑1 (MQ=255)
                                                      ccacCGCAAAACCGCGCCCTTGCTCACCCGCACGCGCAGCCTCAACCGCCGCGTTCAAAGCCAGAATATTg              >  1:2145271/1‑71 (MQ=255)
                                                        acTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCa                            >  1:872275/1‑55 (MQ=255)
                                                        acTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGt            >  1:1418717/1‑71 (MQ=255)
                                                         cTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTc                                          <  1:983823/40‑1 (MQ=255)
                                                         cTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTc           >  1:4854925/1‑71 (MQ=255)
                                                            cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAg                           >  1:560931/1‑52 (MQ=255)
                                                            cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTg         <  1:3944400/70‑1 (MQ=255)
                                                             aaaaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAata                 >  1:5113625/1‑61 (MQ=255)
                                                             aaaaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAata                 >  1:915605/1‑61 (MQ=255)
                                                               aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTc                             <  1:4460276/47‑1 (MQ=255)
                                                               aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc     <  1:310011/71‑1 (MQ=255)
                                                               aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc     >  1:2298716/1‑71 (MQ=255)
                                                               aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc     <  1:210974/71‑1 (MQ=255)
                                                               aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc     <  1:3913188/71‑1 (MQ=255)
                                                                 ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACg    >  1:2514079/1‑70 (MQ=255)
                                                                 ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGc   <  1:1503705/71‑1 (MQ=255)
                                                                 ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGc   <  1:61465/71‑1 (MQ=255)
                                                                  cTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCa  <  1:3548251/71‑1 (MQ=255)
                                                                     ggCCCTGCTCGCCGGCGCGCGCCGCTTCGACcgccgc                                 >  1:1004657/2‑37 (MQ=25)
                                                                      |                                                                  
TATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCA  >  Exported/2068571‑2068707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: