Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 2,068,647 | T→C | 20.6% | E381E (GAA→GAG) | aer ← | fused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 2,068,647 | 0 | T | C | 20.6% | 121.1 / 26.9 | 63 | E381E (GAA→GAG) | aer | fused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component |
Reads supporting (aligned to +/- strand): ref base T (27/23); new base C (7/6); total (34/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01 |
GGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAA > Exported/2068578‑2068717 | ggCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGtt > 1:1260742/1‑71 (MQ=255) ggCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGtt > 1:2271021/1‑71 (MQ=255) aTTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCgg < 1:1934680/71‑1 (MQ=255) ttAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGcc < 1:2562609/68‑1 (MQ=255) ttAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCgg < 1:4988295/70‑1 (MQ=255) tAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCa > 1:4627060/1‑71 (MQ=255) aGCGCTGCGGCTGGCTAAATGCCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCg > 1:3394627/1‑67 (MQ=255) aGCGCTGCGGCTGGCTAAATGCCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCAc > 1:1497492/1‑71 (MQ=255) aGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCg > 1:288336/1‑67 (MQ=255) cgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCAcgc < 1:2795825/71‑1 (MQ=255) gcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCAcgcg < 1:3653829/71‑1 (MQ=255) cTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACgcgc < 1:31476/71‑1 (MQ=255) tacGGCTGGCAAGATTGCGCACTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCgagc > 1:949004/3‑70 (MQ=255) cGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGc > 1:2561294/1‑58 (MQ=255) ctggctAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCGTc > 1:5514198/1‑71 (MQ=255) ctAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc < 1:5691084/49‑1 (MQ=255) aaaTGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc < 1:96151/47‑1 (MQ=255) aTGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGt > 1:591358/1‑43 (MQ=255) aTGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGc > 1:3498637/1‑47 (MQ=255) tGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAccgccg > 1:2579055/1‑70 (MQ=255) tGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAccgcc > 1:4293917/1‑69 (MQ=255) aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGcgcc < 1:3152915/55‑1 (MQ=255) aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGcgcc < 1:3223092/55‑1 (MQ=255) aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGcgcc < 1:5137532/55‑1 (MQ=255) cGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt < 1:4839485/71‑1 (MQ=255) ttCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAggg > 1:4406751/1‑71 (MQ=255) tcaccTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCa > 1:3808880/4‑67 (MQ=255) tcaccTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGc > 1:1607488/4‑71 (MQ=255) tCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCa > 1:3851941/1‑55 (MQ=255) tCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCa > 1:5501552/1‑55 (MQ=255) ccccTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGc > 1:3133454/1‑70 (MQ=255) ccTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAg < 1:3115023/71‑1 (MQ=255) tGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt < 1:1804907/60‑1 (MQ=255) tGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa > 1:4876798/1‑71 (MQ=255) gCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGaa > 1:551201/1‑70 (MQ=255) gCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa > 1:3128799/1‑70 (MQ=255) ccactaCCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGaa < 1:1596229/69‑1 (MQ=255) ccaccaCTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAc > 1:1004843/1‑49 (MQ=255) caccacTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat < 1:3381062/71‑1 (MQ=255) caccacAGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat > 1:1366067/1‑71 (MQ=255) ccacTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTg < 1:3391711/71‑1 (MQ=255) ccacCGCAAAACCGCGCCCTTGCTCACCCGCACGCGCAGCCTCAACCGCCGCGTTCAAAGCCAGAATATTg < 1:5665247/71‑1 (MQ=255) ccacCGCAAAACCGCGCCCTTGCTCACCCGCACGCGCAGCCTCAACCGCCGCGTTCAAAGCCAGAATATTg > 1:2145271/1‑71 (MQ=255) acTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCa > 1:872275/1‑55 (MQ=255) acTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGt > 1:1418717/1‑71 (MQ=255) cTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTc < 1:983823/40‑1 (MQ=255) cTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTc > 1:4854925/1‑71 (MQ=255) cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAg > 1:560931/1‑52 (MQ=255) cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTg < 1:3944400/70‑1 (MQ=255) aaaaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAata > 1:5113625/1‑61 (MQ=255) aaaaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAata > 1:915605/1‑61 (MQ=255) aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTc < 1:4460276/47‑1 (MQ=255) aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc < 1:3913188/71‑1 (MQ=255) aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc < 1:210974/71‑1 (MQ=255) aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc < 1:310011/71‑1 (MQ=255) aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc > 1:2298716/1‑71 (MQ=255) ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACg > 1:2514079/1‑70 (MQ=255) ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGc < 1:1503705/71‑1 (MQ=255) ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGc < 1:61465/71‑1 (MQ=255) cTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCa < 1:3548251/71‑1 (MQ=255) ggCCCTGCTCGCCGGCGCGCGCCGCTTCGACcgccgc > 1:1004657/2‑37 (MQ=25) cccTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGAATATTGGTCTGGAAAGCAattcc < 1:2684443/71‑4 (MQ=255) ccTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCg > 1:5478507/1‑71 (MQ=255) cTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt < 1:74979/36‑1 (MQ=16) tGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGt > 1:5684660/1‑70 (MQ=255) gTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGtt > 1:4153744/1‑70 (MQ=255) ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATAtt > 1:4971986/1‑48 (MQ=255) ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATAtt > 1:1426970/1‑48 (MQ=255) ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTaa > 1:3250491/1‑71 (MQ=255) ttCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTaa < 1:3532087/71‑1 (MQ=255) | GGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCAATATCGTTAA > Exported/2068578‑2068717 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |