| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,076,941 | C→T | 17.8% | intergenic (+57/+88) | ebgC → / ← uxaA | cryptic beta‑D‑galactosidase, beta subunit/altronate hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,076,941 | 0 | C | T | 17.8% | 57.9 / 6.3 | 28 | intergenic (+57/+88) | ebgC/uxaA | cryptic beta‑D‑galactosidase, beta subunit/altronate hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (13/10); new base T (2/3); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
AACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTC > Exported/2076876‑2076986 | aacaaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGtt > 1:696637/1‑71 (MQ=255) acaaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGtta > 1:674936/1‑70 (MQ=255) acaaataacaaCTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGtta > 1:4490368/1‑70 (MQ=255) tACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTGATAAATCCTTAg > 1:996873/1‑71 (MQ=255) tACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTGATAAATCCTTAg > 1:4125962/1‑71 (MQ=255) cggcggCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGATGTGtt < 1:6115/56‑1 (MQ=255) cggcAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTGATAAATCCTTAGCAGGt < 1:2556103/71‑1 (MQ=255) aaaaGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTGATAAATCCTTAGCa > 1:1609770/1‑64 (MQ=255) ggAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTGATAAATCCTTAGCAGGTATGGaa > 1:4520091/1‑69 (MQ=255) gTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTGATAAATCCTTAGCAGGTATGGAAAAGCa < 1:1292725/71‑1 (MQ=255) tttGCCGCCACCGCTACCCTACTCATTTTCGGAGATgtgt < 1:80979/40‑1 (MQ=255) tttGCCGCCACCGCTACCCTACTCATTTTCGGAGATgtgt < 1:4641401/40‑1 (MQ=255) tttGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTGATAAATCCTTAGCAGGTATGGAAAAGCaa < 1:2835140/71‑1 (MQ=255) tGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTGATAAATCCTTAGCAGGTATGGAAAAGCAAAc > 1:1938776/1‑71 (MQ=255) tGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGGTGATaa > 1:1157794/1‑44 (MQ=255) ccGCTACCCTACTCATTTTCGGAGACGTGTTGATAAATCCTTAGCAGGTATGGAAAAGCa < 1:3774690/60‑1 (MQ=255) ccGCTACCCTACTCATTTTCGGAGACGTGTTGATAAATCCTTAGCAGGTATGGAAAAGCa < 1:2510973/60‑1 (MQ=255) ccGCTACCCTACTCATTTTCGGAGACGTGTTGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTc < 1:81953/71‑1 (MQ=255) aCTCATTTTCGGAGACGTGTTGATAAATCCTTAGCAGGTATGGa < 1:3717421/44‑1 (MQ=255) aCTCATTTTCGGAGACGTGTTGATAAATCCTTAGCAGGTATGGa < 1:433317/44‑1 (MQ=255) aCTCATTTTCGGAGACGTGTTGATAAATCCTTAGCAGGTATGGa < 1:2299471/44‑1 (MQ=255) aCTCATTTTCGGAGACGTGTTGATAAATCCTTAGCAGGTATGGa < 1:1486249/44‑1 (MQ=255) aCTCATTTTCGGAGACGTGTTGATAAATCCTTAGCAGGTATGGAAAAGCa > 1:1460200/1‑50 (MQ=255) aCTCATTTTCGGAGACGTGTTGATAAATCCTTAGCAGGTATGGAAAAGCa > 1:2584456/1‑50 (MQ=255) aTTTTCGGAGACGTGTTGATAAATCCTTAGCAGGTATGGaaaa > 1:754749/1‑43 (MQ=255) aTTTTCGGAGACGTGTTGATAAATCCTTAGCAGGTATGGaaa > 1:995356/1‑42 (MQ=255) ttCGGAGACGTGTTGATAAATCCTTAGCAGGTATGGAAAAGCAAACgg > 1:2963432/1‑48 (MQ=255) ttCGGAGACGTGTTGATAAATCCTTAGCAGGTATGGAAAAGCAAACgg > 1:1765498/1‑48 (MQ=255) | AACAAATAACAACTACGGCGGCAAAAGGAGTTTGCCGCCACCGCTACCCTACTCATTTTCGGAGACGTGTTGATAAATCCTTAGCAGGTATGGAAAAGCAAACGGCGCTTC > Exported/2076876‑2076986 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |