Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,093,679	C→T	12.7%	P391P (CCG→CCA)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,093,679	0	C	T	12.7%	240.9 / 19.9	94	P391P (CCG→CCA)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base C (44/38);  new base T (7/5);  total (51/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCG  >  Exported/2093609‑2093749                                                                       |                                                                       aGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCAc                                                                        >  1:3369357/1‑71 (MQ=255) aGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCAc                                                                        >  1:3772774/1‑71 (MQ=255)     gcggcgCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGa                                                                    >  1:3882536/1‑71 (MQ=255)     gcggcgCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGa                                                                    >  1:2599924/1‑71 (MQ=255)           cATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGt                                                               <  1:1185664/70‑1 (MQ=255)            aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                             >  1:5358735/1‑71 (MQ=255)               gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTg                                                          <  1:1356241/71‑1 (MQ=255)               gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTg                                                          >  1:374560/1‑71 (MQ=255)                ccATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                             >  1:1770903/1‑67 (MQ=255)                   tCCTCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAtt                                                                  <  1:5050246/59‑1 (MQ=255)                   tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAt                                                                   <  1:5245108/58‑1 (MQ=255)                   tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTATCTGTCCg                                                      <  1:4469926/71‑1 (MQ=255)                   tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGt                                                         <  1:4249346/68‑1 (MQ=255)                    ccGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                                                                 >  1:3383283/1‑59 (MQ=255)                     cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg                                                     >  1:1237253/1‑70 (MQ=255)                     cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg                                                     >  1:1152682/1‑70 (MQ=255)                     cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg                                                     >  1:602812/1‑70 (MQ=255)                      gtgcggcgTTCACCGCTTTTACTGCCGCAATGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGcg                                                   <  1:140230/69‑2 (MQ=255)                      gtgcggcgTTCACCGCTTTTACTGCCGCAATGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGcg                                                   <  1:2659374/69‑2 (MQ=255)                      gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGc                                                    >  1:4318269/1‑70 (MQ=255)                      gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGc                                                    >  1:367462/1‑70 (MQ=255)                          ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg                                               <  1:3983882/71‑1 (MQ=255)                          ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg                                               <  1:2229390/71‑1 (MQ=255)                           gcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                              <  1:47870/71‑1 (MQ=255)                            cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGa                                             >  1:4124898/1‑71 (MQ=255)                             gTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                            >  1:2398074/1‑71 (MQ=255)                             gTTTACTGCTTACACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                            >  1:4688571/1‑71 (MQ=255)                              tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                                   <  1:1033904/63‑1 (MQ=255)                               ttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAAc                                                 <  1:2483797/64‑1 (MQ=255)                                tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                          >  1:3354777/1‑70 (MQ=255)                                tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                          >  1:1807330/1‑70 (MQ=255)                                tACTGCTTTCACGGCATTAATGGAATTACGTTCAATGCACCGGATTTGTACCTg                                                          >  1:360010/1‑54 (MQ=38)                                 aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACggg                                                                     >  1:2728630/1‑42 (MQ=255)                                 aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAcc                                                            >  1:4997200/1‑51 (MQ=255)                                 aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAcc                                                            >  1:384749/1‑51 (MQ=255)                                 aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                          >  1:428430/1‑69 (MQ=255)                                 aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                          >  1:3965734/1‑69 (MQ=255)                                 aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGc                                         <  1:1246911/70‑1 (MQ=255)                                 aCTGCTTTCACGGCATTAAAGGCATTACGTTCAATGCACGGGATTTGTAcc                                                            >  1:224919/1‑51 (MQ=255)                                 aCTGCTTTCACGGAATTAATGGCATTACGTTCAATGCACggg                                                                     >  1:1392820/1‑42 (MQ=255)                                   tGCTTTCACGGCATTAATGGCATTACGTTCAATGCACgg                                                                      <  1:327343/39‑1 (MQ=255)                                     cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                     <  1:870489/70‑1 (MQ=255)                                       ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                  >  1:3275880/1‑71 (MQ=255)                                        tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGc                                 >  1:5304007/1‑71 (MQ=255)                                           cGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                                   <  1:4757448/50‑1 (MQ=255)                                            ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                             <  1:1316666/71‑1 (MQ=255)                                            ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                             <  1:4238672/71‑1 (MQ=255)                                            ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                             <  1:4417386/71‑1 (MQ=255)                                              cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                           >  1:1290078/1‑71 (MQ=255)                                               aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                          <  1:3518293/71‑1 (MQ=255)                                                  aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                       >  1:62536/1‑71 (MQ=255)                                                   aTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGaccg                              <  1:4700496/63‑2 (MQ=255)                                                    tGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                              <  1:2850388/62‑1 (MQ=255)                                                    tGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                              <  1:4082327/62‑1 (MQ=255)                                                     ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                    >  1:72115/1‑71 (MQ=255)                                                     ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                    >  1:5369122/1‑71 (MQ=255)                                                     ggCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCt                    >  1:2510150/1‑71 (MQ=255)                                                      gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                       >  1:1661883/1‑51 (MQ=255)                                                         ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                                   <  1:4158560/36‑1 (MQ=255)                                                         ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                           <  1:5595479/60‑1 (MQ=255)                                                         ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                           <  1:1899907/60‑1 (MQ=255)                                                         ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                           <  1:1350301/60‑1 (MQ=255)                                                         ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                           <  1:3464394/60‑1 (MQ=255)                                                         ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                       <  1:4430465/64‑1 (MQ=255)                                                         ttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAgg                           <  1:5253238/60‑1 (MQ=255)                                                         ttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAt                <  1:2779624/71‑1 (MQ=255)                                                          tACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                                   <  1:3204794/35‑1 (MQ=255)                                                           aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                     >  1:2178079/1‑48 (MQ=255)                                                           aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTc                                    >  1:511074/1‑49 (MQ=255)                                                           aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTc                                    >  1:121262/1‑49 (MQ=255)                                                           aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                               >  1:4994782/1‑54 (MQ=255)                                                           aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg              >  1:5653179/1‑71 (MQ=255)                                                           aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg              >  1:114394/1‑71 (MQ=255)                                                           aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAgac                                >  1:5432081/1‑51 (MQ=255)                                                           aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGtt                         >  1:605005/1‑60 (MQ=255)                                                           aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                    >  1:346/1‑65 (MQ=255)                                                           aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg              >  1:1677202/1‑69 (MQ=255)                                                           aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg              >  1:944373/1‑69 (MQ=255)                                                            cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc             <  1:1106021/71‑1 (MQ=255)                                                            cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc             <  1:3410395/71‑1 (MQ=255)                                                             gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                     <  1:2960225/46‑1 (MQ=255)                                                                cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                        >  1:4516193/1‑56 (MQ=255)                                                                cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                        >  1:4494426/1‑56 (MQ=255)                                                                cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                        >  1:3693864/1‑56 (MQ=255)                                                                cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt         >  1:3584056/1‑71 (MQ=255)                                                                cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt         >  1:1451747/1‑71 (MQ=255)                                                                cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt         >  1:3590660/1‑71 (MQ=255)                                                                cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAt          <  1:4537438/70‑1 (MQ=255)                                                                 aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt        <  1:1529390/71‑1 (MQ=255)                                                                   tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                          <  1:4681619/51‑1 (MQ=255)                                                                   tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                          <  1:4015058/51‑1 (MQ=255)                                                                   tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                          <  1:226556/51‑1 (MQ=255)                                                                    gCACGGGATTTGTACCTGTCCGGCAACCGGATCGc                                         <  1:1708365/35‑1 (MQ=255)                                                                    gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                       <  1:236864/53‑1 (MQ=255)                                                                     cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc    >  1:1744041/1‑71 (MQ=255)                                                                      aCGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGc                     >  1:216264/1‑53 (MQ=255)                                                                       cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg  <  1:5343954/71‑1 (MQ=255)                                                                       cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg  <  1:381/71‑1 (MQ=255)                                                                       |                                                                       AGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCG  >  Exported/2093609‑2093749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑