BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,093,684	T→C	13.0%	I390V (ATC→GTC) ‡	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,093,684	0	T	C	13.0%	224.5 / 21.3	92	I390V (ATC→GTC) ‡	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand): ref base T (41/39); new base C (7/5); total (48/44)
Fisher's exact test for biased strand distribution p-value = 7.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 101 total aligned reads displayed.

CATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTG  >  Exported/2093619‑2093754

cATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGt                                                                    <  1:1185664/70‑1 (MQ=255)

 aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                                  >  1:5358735/1‑71 (MQ=255)

    gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTg                                                               >  1:374560/1‑71 (MQ=255)

    gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTg                                                               <  1:1356241/71‑1 (MQ=255)

     ccATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                                                                  >  1:1770903/1‑67 (MQ=255)

        tCCTCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAtt                                                                       <  1:5050246/59‑1 (MQ=255)

        tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAt                                                                        <  1:5245108/58‑1 (MQ=255)

        tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTATCTGTCCg                                                           <  1:4469926/71‑1 (MQ=255)

        tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGt                                                              <  1:4249346/68‑1 (MQ=255)

         ccGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                                                                      >  1:3383283/1‑59 (MQ=255)

          cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg                                                          >  1:1152682/1‑70 (MQ=255)

          cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg                                                          >  1:1237253/1‑70 (MQ=255)

          cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg                                                          >  1:602812/1‑70 (MQ=255)

           gtgcggcgTTCACCGCTTTTACTGCCGCAATGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGcg                                                        <  1:2659374/69‑2 (MQ=255)

           gtgcggcgTTCACCGCTTTTACTGCCGCAATGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGcg                                                        <  1:140230/69‑2 (MQ=255)

           gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGc                                                         >  1:4318269/1‑70 (MQ=255)

           gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGc                                                         >  1:367462/1‑70 (MQ=255)

               ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg                                                    <  1:3983882/71‑1 (MQ=255)

               ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg                                                    <  1:2229390/71‑1 (MQ=255)

                gcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg                                                   <  1:47870/71‑1 (MQ=255)

                 cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGa                                                  >  1:4124898/1‑71 (MQ=255)

                  gTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                                 >  1:2398074/1‑71 (MQ=255)

                  gTTTACTGCTTACACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt                                                 >  1:4688571/1‑71 (MQ=255)

                   tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                                        <  1:1033904/63‑1 (MQ=255)

                    ttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAAc                                                      <  1:2483797/64‑1 (MQ=255)

                     tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                               >  1:3354777/1‑70 (MQ=255)

                     tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                               >  1:1807330/1‑70 (MQ=255)

                     tACTGCTTTCACGGCATTAATGGAATTACGTTCAATGCACCGGATTTGTACCTg                                                               >  1:360010/1‑54 (MQ=38)

                      aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAcc                                                                 >  1:384749/1‑51 (MQ=255)

                      aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAcc                                                                 >  1:4997200/1‑51 (MQ=255)

                      aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                               >  1:428430/1‑69 (MQ=255)

                      aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg                                               >  1:3965734/1‑69 (MQ=255)

                      aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGc                                              <  1:1246911/70‑1 (MQ=255)

                      aCTGCTTTCACGGCATTAAAGGCATTACGTTCAATGCACGGGATTTGTAcc                                                                 >  1:224919/1‑51 (MQ=255)

                          cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                          <  1:870489/70‑1 (MQ=255)

                            ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg                                       >  1:3275880/1‑71 (MQ=255)

                             tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGc                                      >  1:5304007/1‑71 (MQ=255)

                                cGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                                        <  1:4757448/50‑1 (MQ=255)

                                 ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                  <  1:4238672/71‑1 (MQ=255)

                                 ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                  <  1:1316666/71‑1 (MQ=255)

                                 ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                  <  1:4417386/71‑1 (MQ=255)

                                   cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                >  1:1290078/1‑71 (MQ=255)

                                    aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                               <  1:3518293/71‑1 (MQ=255)

                                       aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                            >  1:62536/1‑71 (MQ=255)

                                        aTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGaccg                                   <  1:4700496/63‑2 (MQ=255)

                                         tGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                                   <  1:4082327/62‑1 (MQ=255)

                                         tGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                                   <  1:2850388/62‑1 (MQ=255)

                                          ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                         >  1:72115/1‑71 (MQ=255)

                                          ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                         >  1:5369122/1‑71 (MQ=255)

                                          ggCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCt                         >  1:2510150/1‑71 (MQ=255)

                                           gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg                                            >  1:1661883/1‑51 (MQ=255)

                                              ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                                        <  1:4158560/36‑1 (MQ=255)

                                              ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                <  1:1899907/60‑1 (MQ=255)

                                              ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                <  1:1350301/60‑1 (MQ=255)

                                              ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                <  1:3464394/60‑1 (MQ=255)

                                              ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                <  1:5595479/60‑1 (MQ=255)

                                              ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                            <  1:4430465/64‑1 (MQ=255)

                                              ttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAgg                                <  1:5253238/60‑1 (MQ=255)

                                              ttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAt                     <  1:2779624/71‑1 (MQ=255)

                                               tACGTTCAATGCACGGGATTTGTACCTGTCCGGCa                                                        <  1:3204794/35‑1 (MQ=255)

                                                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                          >  1:2178079/1‑48 (MQ=255)

                                                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTc                                         >  1:511074/1‑49 (MQ=255)

                                                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTc                                         >  1:121262/1‑49 (MQ=255)

                                                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc                                    >  1:4994782/1‑54 (MQ=255)

                                                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                   >  1:5653179/1‑71 (MQ=255)

                                                aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                   >  1:114394/1‑71 (MQ=255)

                                                aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAgac                                     >  1:5432081/1‑51 (MQ=255)

                                                aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGtt                              >  1:605005/1‑60 (MQ=255)

                                                aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                         >  1:346/1‑65 (MQ=255)

                                                aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                   >  1:1677202/1‑69 (MQ=255)

                                                 cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                  <  1:1106021/71‑1 (MQ=255)

                                                 cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                  <  1:3410395/71‑1 (MQ=255)

                                                  gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt                                          <  1:2960225/46‑1 (MQ=255)

                                                     cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                             >  1:4516193/1‑56 (MQ=255)

                                                     cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                             >  1:4494426/1‑56 (MQ=255)

                                                     cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                             >  1:3693864/1‑56 (MQ=255)

                                                     cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt              >  1:3584056/1‑71 (MQ=255)

                                                     cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt              >  1:3590660/1‑71 (MQ=255)

                                                     cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt              >  1:1451747/1‑71 (MQ=255)

                                                     cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAt               <  1:4537438/70‑1 (MQ=255)

                                                      aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt             <  1:1529390/71‑1 (MQ=255)

                                                        tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                               <  1:4015058/51‑1 (MQ=255)

                                                        tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                               <  1:4681619/51‑1 (MQ=255)

                                                        tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                               <  1:226556/51‑1 (MQ=255)

                                                         gCACGGGATTTGTACCTGTCCGGCAACCGGATCGc                                              <  1:1708365/35‑1 (MQ=255)

                                                         gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                            <  1:236864/53‑1 (MQ=255)

                                                          cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc         >  1:1744041/1‑71 (MQ=255)

                                                           aCGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGc                          >  1:216264/1‑53 (MQ=255)

                                                            cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg       <  1:381/71‑1 (MQ=255)

                                                            cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg       <  1:5343954/71‑1 (MQ=255)

                                                             gggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc           >  1:1219149/1‑66 (MQ=255)

                                                               gATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                            <  1:4799612/47‑1 (MQ=255)

                                                                aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                             >  1:2327529/1‑45 (MQ=255)

                                                                aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                             <  1:686516/45‑1 (MQ=255)

                                                                 tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCGAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg  >  1:1978175/1‑71 (MQ=255)

                                                                 tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                            <  1:3246839/45‑1 (MQ=255)

                                                                 tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                            <  1:3523781/45‑1 (MQ=255)

                                                                 tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                       <  1:20459/50‑1 (MQ=255)

                                                                 tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                       <  1:5240538/50‑1 (MQ=255)

                                                                 tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                       <  1:4156908/50‑1 (MQ=255)

CATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTG  >  Exported/2093619‑2093754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`