Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,093,727	A→G	13.6%	H375H (CAT→CAC)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,093,727	0	A	G	13.6%	220.3 / 21.2	88	H375H (CAT→CAC)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base A (38/38);  new base G (10/2);  total (48/40)
Fisher's exact test for biased strand distribution p-value = 5.78e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACC  >  Exported/2093658‑2093794                                                                      |                                                                    aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                                    >  1:62536/1‑71 (MQ=255)    ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                                                                 >  1:72115/1‑71 (MQ=255)    ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                                                                 >  1:5369122/1‑71 (MQ=255)    ggCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCt                                                                 >  1:2510150/1‑71 (MQ=255)        ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                                    <  1:4430465/64‑1 (MQ=255)        ttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAt                                                             <  1:2779624/71‑1 (MQ=255)          aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                           >  1:114394/1‑71 (MQ=255)          aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                           >  1:5653179/1‑71 (MQ=255)          aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                                                                 >  1:346/1‑65 (MQ=255)          aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                                           >  1:944373/1‑69 (MQ=255)          aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                                           >  1:1677202/1‑69 (MQ=255)           cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                                          <  1:1106021/71‑1 (MQ=255)           cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                                          <  1:3410395/71‑1 (MQ=255)               cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                     >  1:4516193/1‑56 (MQ=255)               cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                     >  1:4494426/1‑56 (MQ=255)               cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                     >  1:3693864/1‑56 (MQ=255)               cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                                      >  1:1451747/1‑71 (MQ=255)               cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                                      >  1:3584056/1‑71 (MQ=255)               cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                                      >  1:3590660/1‑71 (MQ=255)               cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAt                                                       <  1:4537438/70‑1 (MQ=255)                aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt                                                     <  1:1529390/71‑1 (MQ=255)                   gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                                    <  1:236864/53‑1 (MQ=255)                    cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                                 >  1:1744041/1‑71 (MQ=255)                     aCGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGc                                                                  >  1:216264/1‑53 (MQ=255)                      cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg                                               <  1:381/71‑1 (MQ=255)                      cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg                                               <  1:5343954/71‑1 (MQ=255)                       gggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                                   >  1:1219149/1‑66 (MQ=255)                         gATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                                    <  1:4799612/47‑1 (MQ=255)                          aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                     >  1:2327529/1‑45 (MQ=255)                          aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                     <  1:686516/45‑1 (MQ=255)                           tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCGAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                          >  1:1978175/1‑71 (MQ=255)                           tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                                    <  1:3246839/45‑1 (MQ=255)                           tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                                    <  1:3523781/45‑1 (MQ=255)                           tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                                                               <  1:5240538/50‑1 (MQ=255)                           tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                                                               <  1:20459/50‑1 (MQ=255)                           tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                                                               <  1:4156908/50‑1 (MQ=255)                            ttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                          >  1:5319644/1‑70 (MQ=255)                             tGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCa                                                              >  1:3967197/1‑49 (MQ=255)                             tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCAt                                                             >  1:2946154/1‑50 (MQ=255)                              gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTtgtg                                                                   >  1:5605557/1‑43 (MQ=255)                              gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCa                                        >  1:2198475/1‑70 (MQ=12)                              gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCa                                        >  1:2405313/1‑70 (MQ=12)                              gTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                                        <  1:1369682/70‑1 (MQ=255)                                 ccTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAcc                                    >  1:885311/1‑71 (MQ=255)                                  cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGTGATTTCCGCCGCATTGCAt                                       <  1:774486/67‑1 (MQ=255)                                  cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                                            >  1:1351605/1‑62 (MQ=255)                                  cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                                            >  1:3664762/1‑62 (MQ=255)                                  cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                       <  1:2939965/67‑1 (MQ=255)                                    gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAc                                     >  1:810687/1‑67 (MQ=255)                                     tCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTgcg                                >  1:2494244/1‑71 (MQ=255)                                     tCCGGCAACCGGATCGCAGGTCAGGCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgc                                 >  1:2755031/1‑70 (MQ=255)                                     tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt                                                     >  1:206288/1‑50 (MQ=255)                                     tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt                                                     >  1:2369391/1‑50 (MQ=255)                                     tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt                                                     >  1:4332631/1‑50 (MQ=255)                                     tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                          <  1:3523794/61‑1 (MQ=255)                                     tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                          <  1:5115985/61‑1 (MQ=255)                                     tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                          <  1:1704363/61‑1 (MQ=255)                                     tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                          <  1:1450476/61‑1 (MQ=255)                                     tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                       <  1:1091913/64‑1 (MQ=255)                                     tCCGCCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                       <  1:5143837/64‑1 (MQ=255)                                      ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                                 >  1:3931271/1‑53 (MQ=255)                                      ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                                 >  1:5102481/1‑53 (MQ=255)                                      ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                                 >  1:2417862/1‑53 (MQ=255)                                      ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                                 >  1:5033261/1‑53 (MQ=255)                                      ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                                 >  1:3493960/1‑53 (MQ=255)                                       cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt                                                     >  1:1296817/1‑48 (MQ=255)                                       cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                                        >  1:4030355/1‑61 (MQ=255)                                       cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGcc                              >  1:5285012/1‑71 (MQ=255)                                       cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGcc                              >  1:1777827/1‑71 (MQ=255)                                         gcgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCgg                            <  1:5587325/68‑1 (MQ=9)                                         gcgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCgg                            >  1:3939444/4‑71 (MQ=9)                                               ggATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTg                                  >  1:3450218/1‑59 (MQ=255)                                                 aTCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTgccgc                    <  1:3127635/71‑1 (MQ=255)                                                    gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                                   >  1:2162419/1‑37 (MQ=255)                                                    gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                            <  1:1952331/60‑1 (MQ=255)                                                    gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                            <  1:3908258/60‑1 (MQ=255)                                                    gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                            <  1:2574925/60‑1 (MQ=255)                                                    gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                            <  1:4934977/60‑1 (MQ=255)                                                    gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCa                 <  1:3822503/71‑1 (MQ=255)                                                     cAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCag                <  1:3440753/71‑1 (MQ=255)                                                         tCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagtagt            <  1:3609381/71‑1 (MQ=255)                                                         gcAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                          <  1:5623993/40‑1 (MQ=255)                                                           aGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGtt           <  1:577449/70‑1 (MQ=255)                                                            gCCCAAGGTTATGCTCCATCGCGATTTCCGCCGGATAGCATAc                                     >  1:322804/1‑43 (MQ=38)                                                               cAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGt       >  1:556013/1‑70 (MQ=255)                                                                aaGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTa     <  1:1068627/71‑1 (MQ=255)                                                                aaGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTa     <  1:1061863/71‑1 (MQ=255)                                                                 aGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTaa    <  1:3242207/71‑1 (MQ=255)                                                                  ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg        >  1:1757215/1‑66 (MQ=255)                                                                  ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg        >  1:80479/1‑66 (MQ=255)                                                                  ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg        >  1:2795890/1‑66 (MQ=255)                                                                  ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg        >  1:2639012/1‑66 (MQ=255)                                                                   gTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc  <  1:4757352/71‑1 (MQ=255)                                                                   gTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc  <  1:829348/71‑1 (MQ=255)                                                                      |                                                                    AATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACC  >  Exported/2093658‑2093794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑