Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,093,752 T→A 11.5% N367I (AAT→ATT) ‡ tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,093,7520TA11.5% 163.5 / 11.9 61N367I (AAT→ATT) ‡tdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base T (26/28);  new base A (5/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGC  >  Exported/2093684‑2093821
                                                                    |                                                                     
tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCGAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                                                     >  1:1978175/1‑71 (MQ=255)
 ttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                                                     >  1:5319644/1‑70 (MQ=255)
   gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCa                                                                   >  1:2405313/1‑70 (MQ=12)
   gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCa                                                                   >  1:2198475/1‑70 (MQ=12)
   gTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                                                                   <  1:1369682/70‑1 (MQ=255)
      ccTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAcc                                                               >  1:885311/1‑71 (MQ=255)
       cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGTGATTTCCGCCGCATTGCAt                                                                  <  1:774486/67‑1 (MQ=255)
       cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                                                                       >  1:3664762/1‑62 (MQ=255)
       cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                                                                       >  1:1351605/1‑62 (MQ=255)
       cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                                                  <  1:2939965/67‑1 (MQ=255)
         gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAc                                                                >  1:810687/1‑67 (MQ=255)
          tCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTgcg                                                           >  1:2494244/1‑71 (MQ=255)
          tCCGGCAACCGGATCGCAGGTCAGGCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgc                                                            >  1:2755031/1‑70 (MQ=255)
          tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                                                     <  1:1704363/61‑1 (MQ=255)
          tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                                                     <  1:3523794/61‑1 (MQ=255)
          tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                                                     <  1:5115985/61‑1 (MQ=255)
          tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                                                     <  1:1450476/61‑1 (MQ=255)
          tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                                                  <  1:1091913/64‑1 (MQ=255)
          tCCGCCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                                                  <  1:5143837/64‑1 (MQ=255)
            cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                                                                   >  1:4030355/1‑61 (MQ=255)
            cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGcc                                                         >  1:1777827/1‑71 (MQ=255)
            cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGcc                                                         >  1:5285012/1‑71 (MQ=255)
              gcgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCgg                                                       >  1:3939444/4‑71 (MQ=9)
              gcgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCgg                                                       <  1:5587325/68‑1 (MQ=9)
                    ggATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTg                                                             >  1:3450218/1‑59 (MQ=255)
                      aTCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTgccgc                                               <  1:3127635/71‑1 (MQ=255)
                         gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                                                       <  1:4934977/60‑1 (MQ=255)
                         gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                                                       <  1:1952331/60‑1 (MQ=255)
                         gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                                                       <  1:3908258/60‑1 (MQ=255)
                         gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                                                       <  1:2574925/60‑1 (MQ=255)
                         gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCa                                            <  1:3822503/71‑1 (MQ=255)
                          cAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCag                                           <  1:3440753/71‑1 (MQ=255)
                              tCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagtagt                                       <  1:3609381/71‑1 (MQ=255)
                              gcAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                                                     <  1:5623993/40‑1 (MQ=255)
                                aGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGtt                                      <  1:577449/70‑1 (MQ=255)
                                 gCCCAAGGTTATGCTCCATCGCGATTTCCGCCGGATAGCATAc                                                                >  1:322804/1‑43 (MQ=38)
                                    cAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGt                                  >  1:556013/1‑70 (MQ=255)
                                     aaGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTa                                <  1:1061863/71‑1 (MQ=255)
                                     aaGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTa                                <  1:1068627/71‑1 (MQ=255)
                                      aGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTaa                               <  1:3242207/71‑1 (MQ=255)
                                       ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg                                   >  1:80479/1‑66 (MQ=255)
                                       ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg                                   >  1:2795890/1‑66 (MQ=255)
                                       ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg                                   >  1:1757215/1‑66 (MQ=255)
                                       ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg                                   >  1:2639012/1‑66 (MQ=255)
                                        gTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                             <  1:829348/71‑1 (MQ=255)
                                        gTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                             <  1:4757352/71‑1 (MQ=255)
                                                 cATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccg                    >  1:5469700/1‑71 (MQ=255)
                                                  aTCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgc                   >  1:3856781/1‑71 (MQ=255)
                                                   tCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgc                   >  1:2843138/1‑70 (MQ=255)
                                                    cgcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagta                                         >  1:4412515/1‑47 (MQ=255)
                                                     gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGtt                                 >  1:682396/1‑54 (MQ=255)
                                                     gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                             >  1:2489800/1‑58 (MQ=255)
                                                     gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                             >  1:5362224/1‑58 (MQ=255)
                                                     gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                             >  1:1375060/1‑58 (MQ=255)
                                                     gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCAt                >  1:849638/1‑71 (MQ=255)
                                                       gATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCAtt               >  1:4173286/1‑70 (MQ=255)
                                                       gATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTg              <  1:4048256/71‑1 (MQ=255)
                                                        aTTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTc                                     <  1:1469940/47‑1 (MQ=255)
                                                         tttCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgc                   <  1:2904729/64‑1 (MQ=255)
                                                            ccgccgCGATGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGagc           <  1:4323993/69‑3 (MQ=255)
                                                            ccgccgCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACa          <  1:161852/70‑1 (MQ=255)
                                                             cgccgcGATGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGAGCacg        >  1:4570746/1‑69 (MQ=255)
                                                                  cATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCa                 >  1:2495466/1‑57 (MQ=255)
                                                                    ttGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGc  >  1:4963297/1‑70 (MQ=255)
                                                                    |                                                                     
TTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGC  >  Exported/2093684‑2093821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑