Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,093,778 C→T 8.0% L358L (CTG→CTA) ‡ tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,093,7780CT 8.0% 139.7 / 2.4 50L358L (CTG→CTA) ‡tdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base C (25/21);  new base T (2/2);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

GCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCC  >  Exported/2093709‑2093847
                                                                     |                                                                     
gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCa                                                                      <  1:3822503/71‑1 (MQ=255)
 cAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCag                                                                     <  1:3440753/71‑1 (MQ=255)
     tCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagtagt                                                                 <  1:3609381/71‑1 (MQ=255)
       aGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGtt                                                                <  1:577449/70‑1 (MQ=255)
           cAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGt                                                            >  1:556013/1‑70 (MQ=255)
            aaGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTa                                                          <  1:1068627/71‑1 (MQ=255)
            aaGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTa                                                          <  1:1061863/71‑1 (MQ=255)
             aGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTaa                                                         <  1:3242207/71‑1 (MQ=255)
              ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg                                                             >  1:1757215/1‑66 (MQ=255)
              ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg                                                             >  1:2639012/1‑66 (MQ=255)
              ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg                                                             >  1:2795890/1‑66 (MQ=255)
              ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg                                                             >  1:80479/1‑66 (MQ=255)
               gTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                                                       <  1:4757352/71‑1 (MQ=255)
               gTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                                                       <  1:829348/71‑1 (MQ=255)
                        cATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccg                                              >  1:5469700/1‑71 (MQ=255)
                         aTCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgc                                             >  1:3856781/1‑71 (MQ=255)
                          tCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgc                                             >  1:2843138/1‑70 (MQ=255)
                           cgcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagta                                                                   >  1:4412515/1‑47 (MQ=255)
                            gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGtt                                                           >  1:682396/1‑54 (MQ=255)
                            gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                                                       >  1:2489800/1‑58 (MQ=255)
                            gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                                                       >  1:5362224/1‑58 (MQ=255)
                            gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                                                       >  1:1375060/1‑58 (MQ=255)
                            gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCAt                                          >  1:849638/1‑71 (MQ=255)
                              gATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCAtt                                         >  1:4173286/1‑70 (MQ=255)
                              gATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTg                                        <  1:4048256/71‑1 (MQ=255)
                               aTTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTc                                                               <  1:1469940/47‑1 (MQ=255)
                                tttCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgc                                             <  1:2904729/64‑1 (MQ=255)
                                   ccgccgCGATGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGagc                                     <  1:4323993/69‑3 (MQ=255)
                                   ccgccgCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACa                                    <  1:161852/70‑1 (MQ=255)
                                    cgccgcGATGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGAGCacg                                  >  1:4570746/1‑69 (MQ=255)
                                         cATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCa                                           >  1:2495466/1‑57 (MQ=255)
                                           ttGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGc                            >  1:4963297/1‑70 (MQ=255)
                                            tGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGAGCAcgccacgcca                          <  1:543722/71‑1 (MQ=255)
                                             gCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg                                                             >  1:2572045/1‑35 (MQ=255)
                                             gCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAAc                                     >  1:1229003/1‑59 (MQ=255)
                                             gCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCa                          <  1:4668797/70‑1 (MQ=255)
                                             gCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCa                          <  1:4730031/70‑1 (MQ=255)
                                                 aCCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGAGCACGCCACGCCaactt                      >  1:3027797/1‑67 (MQ=255)
                                                   cTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTccc                   >  1:4067880/1‑71 (MQ=255)
                                                      cgcCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAAtc                       >  1:5441428/1‑64 (MQ=255)
                                                      cgcCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCt                <  1:756842/71‑1 (MQ=255)
                                                      cgcCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCt                <  1:4555033/71‑1 (MQ=255)
                                                        ccGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGa              <  1:344475/71‑1 (MQ=255)
                                                        ccGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGa              <  1:663082/71‑1 (MQ=255)
                                                          ggACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGaa                                      >  1:3686379/1‑45 (MQ=255)
                                                          ggACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACa            <  1:1509830/71‑1 (MQ=255)
                                                           gACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAg           >  1:258567/1‑71 (MQ=255)
                                                               gccgccCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCg        >  1:2350007/1‑70 (MQ=255)
                                                                ccgccCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGa       <  1:384699/70‑1 (MQ=255)
                                                                ccgccCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGa       >  1:2655589/1‑70 (MQ=255)
                                                                    ccAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTcc  <  1:995537/71‑1 (MQ=255)
                                                                     cAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGc                            >  1:552915/1‑44 (MQ=255)
                                                                     cAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGc                            >  1:3114831/1‑44 (MQ=255)
                                                                     |                                                                     
GCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCC  >  Exported/2093709‑2093847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑