| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,093,792 | A→G | 7.5% | L354L (TTA→CTA) ‡ | tdcG ← | L‑serine dehydratase 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,093,792 | 0 | A | G | 7.5% | 136.4 / 2.6 | 53 | L354L (TTA→CTA) ‡ | tdcG | L‑serine dehydratase 3 |
| Reads supporting (aligned to +/- strand): ref base A (30/19); new base G (2/2); total (32/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.69e-01 | |||||||||||
AGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGG > Exported/2093722‑2093860 | aGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTaa < 1:3242207/71‑1 (MQ=255) gTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc < 1:829348/71‑1 (MQ=255) gTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc < 1:4757352/71‑1 (MQ=255) cATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccg > 1:5469700/1‑71 (MQ=255) aTCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgc > 1:3856781/1‑71 (MQ=255) tCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgc > 1:2843138/1‑70 (MQ=255) gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc > 1:1375060/1‑58 (MQ=255) gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc > 1:5362224/1‑58 (MQ=255) gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc > 1:2489800/1‑58 (MQ=255) gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCAt > 1:849638/1‑71 (MQ=255) gATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCAtt > 1:4173286/1‑70 (MQ=255) gATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTg < 1:4048256/71‑1 (MQ=255) tttCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgc < 1:2904729/64‑1 (MQ=255) ccgccgCGATGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGagc < 1:4323993/69‑3 (MQ=255) ccgccgCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACa < 1:161852/70‑1 (MQ=255) cgccgcGATGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGAGCacg > 1:4570746/1‑69 (MQ=255) cATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCa > 1:2495466/1‑57 (MQ=255) ttGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGc > 1:4963297/1‑70 (MQ=255) tGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGAGCAcgccacgcca < 1:543722/71‑1 (MQ=255) gCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAAc > 1:1229003/1‑59 (MQ=255) gCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCa < 1:4668797/70‑1 (MQ=255) gCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCa < 1:4730031/70‑1 (MQ=255) aCCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGAGCACGCCACGCCaactt > 1:3027797/1‑67 (MQ=255) cTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTccc > 1:4067880/1‑71 (MQ=255) cgcCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAAtc > 1:5441428/1‑64 (MQ=255) cgcCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCt < 1:4555033/71‑1 (MQ=255) cgcCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCt < 1:756842/71‑1 (MQ=255) ccGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGa < 1:344475/71‑1 (MQ=255) ccGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGa < 1:663082/71‑1 (MQ=255) ggACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGaa > 1:3686379/1‑45 (MQ=255) ggACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACa < 1:1509830/71‑1 (MQ=255) gACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAg > 1:258567/1‑71 (MQ=255) gccgccCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCg > 1:2350007/1‑70 (MQ=255) ccgccCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGa < 1:384699/70‑1 (MQ=255) ccgccCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGa > 1:2655589/1‑70 (MQ=255) ccAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTcc < 1:995537/71‑1 (MQ=255) cAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGc > 1:552915/1‑44 (MQ=255) cAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGc > 1:3114831/1‑44 (MQ=255) agtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCa < 1:4597969/42‑1 (MQ=255) gtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCAc > 1:998413/1‑37 (MQ=255) gtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCAc > 1:1670102/1‑37 (MQ=255) gtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCAc > 1:3106350/1‑37 (MQ=255) gtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAAt > 1:756109/1‑43 (MQ=255) gtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAAt > 1:5150474/1‑43 (MQ=255) gtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCgcgc > 1:3478369/1‑69 (MQ=255) gtTCAGTTAACACTGCCGCCGCCATTGAACAGGCCAc > 1:5085282/1‑37 (MQ=255) ttCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGa < 1:2961925/59‑1 (MQ=255) ttCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTc < 1:1246004/63‑1 (MQ=255) tCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACa < 1:4327679/53‑1 (MQ=255) cAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCaga < 1:155535/70‑1 (MQ=255) cAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCa > 1:5442082/1‑68 (MQ=255) cAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCa > 1:1812151/1‑68 (MQ=255) cAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCa > 1:4780405/1‑68 (MQ=255) cAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCa > 1:4659274/1‑68 (MQ=255) aaCCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCa > 1:2918784/1‑63 (MQ=255) aaCCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAgaga > 1:3914397/1‑67 (MQ=255) aaCCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAgaga > 1:4206147/1‑67 (MQ=255) aaCCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATgg < 1:2754508/70‑1 (MQ=255) aaCCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATg > 1:2734480/1‑69 (MQ=255) | AGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGG > Exported/2093722‑2093860 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |