Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,093,796 T→A 7.4% A352A (GCA→GCT)  tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,093,7960TA 7.4% 152.4 / 3.8 54A352A (GCA→GCT) tdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base T (31/19);  new base A (2/2);  total (33/21)
Fisher's exact test for biased strand distribution p-value = 6.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

CATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGC  >  Exported/2093733‑2093864
                                                               |                                                                    
cATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccg                                                               >  1:5469700/1‑71 (MQ=255)
 aTCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgc                                                              >  1:3856781/1‑71 (MQ=255)
  tCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgc                                                              >  1:2843138/1‑70 (MQ=255)
    gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCAt                                                           >  1:849638/1‑71 (MQ=255)
      gATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCAtt                                                          >  1:4173286/1‑70 (MQ=255)
      gATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTg                                                         <  1:4048256/71‑1 (MQ=255)
        tttCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgc                                                              <  1:2904729/64‑1 (MQ=255)
           ccgccgCGATGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGagc                                                      <  1:4323993/69‑3 (MQ=255)
           ccgccgCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACa                                                     <  1:161852/70‑1 (MQ=255)
            cgccgcGATGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGAGCacg                                                   >  1:4570746/1‑69 (MQ=255)
                 cATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCa                                                            >  1:2495466/1‑57 (MQ=255)
                   ttGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGc                                             >  1:4963297/1‑70 (MQ=255)
                    tGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGAGCAcgccacgcca                                           <  1:543722/71‑1 (MQ=255)
                     gCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAAc                                                      >  1:1229003/1‑59 (MQ=255)
                     gCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCa                                           <  1:4668797/70‑1 (MQ=255)
                     gCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCa                                           <  1:4730031/70‑1 (MQ=255)
                         aCCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGAGCACGCCACGCCaactt                                       >  1:3027797/1‑67 (MQ=255)
                           cTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTccc                                    >  1:4067880/1‑71 (MQ=255)
                              cgcCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAAtc                                        >  1:5441428/1‑64 (MQ=255)
                              cgcCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCt                                 <  1:4555033/71‑1 (MQ=255)
                              cgcCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCt                                 <  1:756842/71‑1 (MQ=255)
                                ccGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGa                               <  1:663082/71‑1 (MQ=255)
                                ccGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGa                               <  1:344475/71‑1 (MQ=255)
                                  ggACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGaa                                                       >  1:3686379/1‑45 (MQ=255)
                                  ggACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACa                             <  1:1509830/71‑1 (MQ=255)
                                   gACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAg                            >  1:258567/1‑71 (MQ=255)
                                       gccgccCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCg                         >  1:2350007/1‑70 (MQ=255)
                                        ccgccCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGa                        <  1:384699/70‑1 (MQ=255)
                                        ccgccCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGa                        >  1:2655589/1‑70 (MQ=255)
                                            ccAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTcc                   <  1:995537/71‑1 (MQ=255)
                                             cAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGc                                             >  1:552915/1‑44 (MQ=255)
                                             cAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGc                                             >  1:3114831/1‑44 (MQ=255)
                                                 agtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCa                                           <  1:4597969/42‑1 (MQ=255)
                                                  gtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCAc                                               >  1:998413/1‑37 (MQ=255)
                                                  gtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCAc                                               >  1:1670102/1‑37 (MQ=255)
                                                  gtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCAc                                               >  1:3106350/1‑37 (MQ=255)
                                                  gtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAAt                                         >  1:756109/1‑43 (MQ=255)
                                                  gtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAAt                                         >  1:5150474/1‑43 (MQ=255)
                                                  gtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCgcgc               >  1:3478369/1‑69 (MQ=255)
                                                  gtTCAGTTAACACTGCCGCCGCCATTGAACAGGCCAc                                               >  1:5085282/1‑37 (MQ=255)
                                                   ttCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGa                        <  1:2961925/59‑1 (MQ=255)
                                                   ttCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTc                    <  1:1246004/63‑1 (MQ=255)
                                                    tCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACa                             <  1:4327679/53‑1 (MQ=255)
                                                     cAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCaga           <  1:155535/70‑1 (MQ=255)
                                                     cAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCa             >  1:4780405/1‑68 (MQ=255)
                                                     cAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCa             >  1:5442082/1‑68 (MQ=255)
                                                     cAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCa             >  1:1812151/1‑68 (MQ=255)
                                                     cAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCa             >  1:4659274/1‑68 (MQ=255)
                                                          aaCCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCa             >  1:2918784/1‑63 (MQ=255)
                                                          aaCCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAgaga         >  1:3914397/1‑67 (MQ=255)
                                                          aaCCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAgaga         >  1:4206147/1‑67 (MQ=255)
                                                          aaCCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATgg      <  1:2754508/70‑1 (MQ=255)
                                                          aaCCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATg       >  1:2734480/1‑69 (MQ=255)
                                                             ccTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGc  <  1:4921248/71‑1 (MQ=255)
                                                               |                                                                    
CATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGC  >  Exported/2093733‑2093864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑