Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,094,015	C→A	16.2%	G279G (GGG→GGT)	tdcG ←	L‑serine dehydratase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,094,015	0	C	A	16.2%	181.4 / 15.1	67	G279G (GGG→GGT)	tdcG	L‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base C (33/23);  new base A (8/3);  total (41/26)
Fisher's exact test for biased strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGA  >  Exported/2093945‑2094084                                                                       |                                                                      aCTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACgcgc                                                                       >  1:3708721/1‑71 (MQ=255) aCTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACgcgc                                                                       >  1:4843/1‑71 (MQ=255)   ttgtCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGcgacc                                                                     >  1:938376/4‑71 (MQ=255)   ttgtCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGcgacc                                                                     >  1:2066948/4‑71 (MQ=255)   ttgtCGTAGTACGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGcgacc                                                                     >  1:5468454/4‑71 (MQ=255)     aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc                                                                   >  1:3695590/1‑71 (MQ=255)     aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc                                                                   >  1:2676583/1‑71 (MQ=255)     aTCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGcgcccgc                                                                   >  1:4637198/1‑71 (MQ=255)        gtagtaCGCCAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAg                                                                >  1:806680/1‑71 (MQ=255)           ataaGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTg                                                             >  1:4459363/1‑71 (MQ=255)              aGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCg                                                           <  1:659736/70‑1 (MQ=255)              aGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGt                                                          >  1:2956915/1‑71 (MQ=255)               gCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCg                                                           <  1:3037432/69‑1 (MQ=255)               gCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGtt                                                         <  1:5166382/71‑1 (MQ=255)               gCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGtt                                                         <  1:465832/71‑1 (MQ=255)                ccAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGttc                                                        <  1:2552742/71‑2 (MQ=255)                ccAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGttc                                                        >  1:2181308/1‑70 (MQ=255)                ccAGAACTGCCGGGATAATCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGttc                                                        >  1:2620699/1‑70 (MQ=255)                 cAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGtttt                                                       <  1:2560447/71‑1 (MQ=255)                 cAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGtttt                                                       <  1:182662/71‑1 (MQ=255)                 cAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGtttt                                                       >  1:1294979/1‑71 (MQ=255)                  aGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttc                                                      >  1:58555/1‑71 (MQ=255)                  aGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttc                                                      >  1:3581412/1‑71 (MQ=255)                    tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt                                                    <  1:3112079/71‑1 (MQ=255)                    tACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt                                                    <  1:4390054/71‑1 (MQ=255)                      cTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt                                                    >  1:1208784/1‑69 (MQ=255)                        gCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGtt                                                         <  1:1173057/62‑1 (MQ=255)                        gCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCAc                                                 >  1:3455443/1‑70 (MQ=255)                          cGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTaa                                              <  1:384785/71‑1 (MQ=255)                           ggAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAAc                                             >  1:3191954/1‑71 (MQ=255)                            ggataatCCCGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGtt                                                         <  1:5387372/56‑1 (MQ=255)                            gAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTaa                                              >  1:4677484/1‑69 (MQ=255)                              ataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGc                                          >  1:5546509/1‑71 (MQ=255)                              ataatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGc                                          >  1:4308065/1‑71 (MQ=255)                                aatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttct                                                     <  1:604785/58‑1 (MQ=255)                                aatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttct                                                     <  1:4361282/58‑1 (MQ=255)                                aatGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttct                                                     <  1:2004588/58‑1 (MQ=255)                                  tcccGCACGCACCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgt                                      >  1:1552554/3‑70 (MQ=255)                                     cgcacgcaCCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCcgcgcg                                   >  1:1346859/1‑71 (MQ=255)                                      gcacgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGcgcg                                   >  1:3312505/1‑70 (MQ=255)                                      gcacgcacCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgca                                   >  1:2334490/1‑69 (MQ=255)                                      gcacgcacCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgca                                   <  1:1044766/70‑2 (MQ=255)                                       cacgcacCGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAgtgcaaa                                 >  1:620203/1‑68 (MQ=255)                                         cgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACa                               >  1:5409053/1‑71 (MQ=255)                                         cgcacCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACa                               >  1:2567839/1‑71 (MQ=255)                                              cGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTg                          <  1:2775499/71‑1 (MQ=255)                                              cGTTAGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAGTGCAAACATGt                            >  1:5522383/1‑69 (MQ=255)                                               gTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAgc                                      >  1:2495112/1‑58 (MQ=255)                                               gTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGcgcg                                   <  1:5421752/61‑1 (MQ=255)                                               gTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGa                         >  1:925203/1‑71 (MQ=255)                                               gTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGa                         >  1:3041413/1‑71 (MQ=255)                                                ttAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                        >  1:466892/1‑71 (MQ=255)                                                ttAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                        >  1:5399221/1‑71 (MQ=255)                                                 tAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGa                         <  1:3601001/69‑1 (MQ=255)                                                  aGTCGGCGCAGTCACCACGCGACCGCCAGCAGCGTTCTCTTCGTTCACTGCCAGTGCAAACATGTTGATc                       >  1:3908176/1‑70 (MQ=255)                                                    tCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttctt                                                    <  1:5136975/39‑1 (MQ=255)                                                      ggTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTc                  >  1:2580445/1‑71 (MQ=255)                                                      ggTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTc                  >  1:749459/1‑71 (MQ=255)                                                          ccGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGAt               >  1:2698284/1‑70 (MQ=255)                                                            gTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGa                >  1:40654/1‑67 (MQ=255)                                                             ttACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACa           <  1:977343/71‑1 (MQ=255)                                                               aCCATGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATcc                      >  1:3007816/1‑58 (MQ=255)                                                               aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt         >  1:3248202/1‑71 (MQ=255)                                                               aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt         >  1:520638/1‑71 (MQ=255)                                                               aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt         >  1:2448947/1‑71 (MQ=255)                                                               aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt         >  1:4967812/1‑71 (MQ=255)                                                               aCCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt         >  1:4415825/1‑71 (MQ=255)                                                                ccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt         <  1:2656370/70‑1 (MQ=255)                                                                ccACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACAtt         <  1:3737987/70‑1 (MQ=255)                                                                 cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                        <  1:5581510/54‑1 (MQ=255)                                                                 cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGAt                        <  1:5061826/54‑1 (MQ=255)                                                                 cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCa       >  1:2046177/1‑71 (MQ=255)                                                                 cACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCa       <  1:72116/71‑1 (MQ=255)                                                                  aCGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCAt      >  1:2703277/1‑71 (MQ=255)                                                                   cgcgCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGa                         >  1:5388240/1‑51 (MQ=255)                                                                      gcccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGa  <  1:5030121/71‑1 (MQ=255)                                                                       cccgccAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATg              <  1:4502263/58‑1 (MQ=255)                                                                       |                                                                      ACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCGATGACATTCATCGGA  >  Exported/2093945‑2094084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑