| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 311,646 | G→C | 6.5% | A409A (GCC→GCG) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 311,646 | 0 | G | C | 6.5% | 244.5 / 4.6 | 77 | A409A (GCC→GCG) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (41/31); new base C (2/3); total (43/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCA > Exported/311585‑311715 | cttcttTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATc < 1:2086679/70‑1 (MQ=255)cttcttTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATc < 1:1499404/70‑1 (MQ=255) ttttGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATccacc > 1:1251386/1‑70 (MQ=255) tGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCaa > 1:1358828/1‑69 (MQ=255) gcggcAAACCTTCTTGCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATccacc > 1:5323789/1‑65 (MQ=255) gcggcAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATccacc > 1:1743876/1‑65 (MQ=255) gcggcAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAgg > 1:951509/1‑71 (MQ=255) gcggcAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAgg > 1:4890893/1‑71 (MQ=255) gcAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGcc > 1:1329531/1‑70 (MQ=255) cAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGa < 1:406510/71‑1 (MQ=255) aaaCCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCa < 1:819344/54‑1 (MQ=255) tcttcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg > 1:109510/1‑71 (MQ=255) tcttcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg > 1:3077261/1‑71 (MQ=255) cttcCGCCATAACACGCTCAACGTTTTCTACCATAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg > 1:5422651/1‑70 (MQ=255) cttcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGat < 1:463952/70‑2 (MQ=255) cttcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg < 1:4820727/70‑1 (MQ=255) cttcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg < 1:1766026/70‑1 (MQ=255) cttcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg < 1:1815962/70‑1 (MQ=255) ttcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATcca > 1:4262650/1‑50 (MQ=255) tcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCg < 1:19042/66‑1 (MQ=255) tcCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCg < 1:59304/66‑1 (MQ=255) cGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATc > 1:2785726/1‑45 (MQ=255) cGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCTATGGCGAGCACCa < 1:538705/71‑1 (MQ=255) gCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCAt < 1:1433740/71‑1 (MQ=255) ccATAACACGCTCAACGTTATCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg < 1:3258236/64‑1 (MQ=255) aTAACATGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATccc > 1:1056061/1‑71 (MQ=255) aTAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATccc > 1:1644918/1‑71 (MQ=255) aTAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATccc > 1:4990330/1‑71 (MQ=255) aTAACACGCTCAACGTTTTCTACAACAACGATGGCGTc > 1:4867762/1‑38 (MQ=255) tAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATcc > 1:458097/1‑41 (MQ=255) tAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATcc > 1:1459547/1‑41 (MQ=255) tAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATcc > 1:2231522/1‑41 (MQ=255) tAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATcc > 1:516200/1‑41 (MQ=255) tAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCg < 1:4198635/71‑1 (MQ=255) tAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCg < 1:3669603/71‑1 (MQ=255) aaCACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCg > 1:3579065/1‑70 (MQ=255) aaCACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCg > 1:3062090/1‑70 (MQ=255) acacGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGaa < 1:252856/71‑1 (MQ=255) acacGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGaa > 1:2018696/1‑71 (MQ=255) acacGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGaa > 1:5234260/1‑71 (MQ=255) acGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACa > 1:3007397/1‑71 (MQ=255) acGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACa > 1:2951861/1‑71 (MQ=255) acGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACa > 1:4959289/1‑71 (MQ=255) gCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACAtt > 1:4596310/1‑71 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATccacca < 1:3480826/37‑1 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATccacca < 1:2937573/37‑1 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATccacca < 1:3967913/37‑1 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATccacca < 1:4555398/37‑1 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAg < 1:2701007/53‑1 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGt > 1:1579717/1‑71 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGt > 1:5357395/1‑71 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGt > 1:4643062/1‑71 (MQ=255) tCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGt > 1:4315453/1‑71 (MQ=255) aCGTTTTCTACCACAACGATGGCGTCATCCACCAACAgg < 1:1445885/39‑1 (MQ=255) cGTTTTCTACCACAACGATGGCGTCATCCCCCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGc > 1:2161141/1‑71 (MQ=255) cGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATg > 1:920907/1‑44 (MQ=255) cGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATg > 1:2560903/1‑44 (MQ=255) cGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATg > 1:2791053/1‑44 (MQ=255) ttttCTACCACAACGATGGCGTCATCCACCAACAGGCCGa < 1:4518397/40‑1 (MQ=255) ttttCTACCACAACGATGGCGTCATCCACCAACAGGCCGa < 1:1845934/40‑1 (MQ=255) ttttCTACCACAACGATGGCGTCATCCACCAACAGGCCGa < 1:1807354/40‑1 (MQ=255) ttttCTACCACAACGATGGCGTCATACACCAACAGGCCGa < 1:4354943/40‑1 (MQ=255) ttCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCgtgt > 1:1397721/1‑71 (MQ=255) ttCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCgtgt > 1:5433983/1‑71 (MQ=255) ttCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCgtg < 1:4225786/70‑1 (MQ=255) tCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCgtgt < 1:3700339/70‑1 (MQ=255) cTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACAt < 1:1941279/58‑1 (MQ=255) caccaccACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGttga > 1:4149736/2‑69 (MQ=9) caccaccACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGttg < 1:4994313/69‑2 (MQ=9) ccaccaCTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGAtg < 1:477367/71‑2 (MQ=2) ccACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATc > 1:1276196/1‑71 (MQ=255) caccacTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGaa > 1:3822069/1‑51 (MQ=2) caccacTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGAtg < 1:5562986/70‑2 (MQ=2) acaACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCga < 1:2423164/71‑1 (MQ=255) caACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCa > 1:2347169/1‑39 (MQ=255) aaCGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACa > 1:3396943/1‑50 (MQ=255) tGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa < 1:1071670/71‑1 (MQ=255) ggCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa > 1:2725324/1‑70 (MQ=255) ggCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa > 1:958283/1‑70 (MQ=255) | CTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCA > Exported/311585‑311715 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |