Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,096,631 C→A 8.3% S103I (AGT→ATT) ‡ garK ← glycerate kinase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,096,6310CA 8.3% 189.9 / 3.2 60S103I (AGT→ATT) ‡garKglycerate kinase I
Reads supporting (aligned to +/- strand):  ref base C (30/25);  new base A (2/3);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 6.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

TACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCA  >  Exported/2096561‑2096699
                                                                      |                                                                    
tACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAAc                                                                      <  1:602647/71‑1 (MQ=255)
   cAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTgg                                                                   <  1:3979976/71‑1 (MQ=255)
         cAGCCCACTGGCGGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCa                                                             <  1:2768444/71‑1 (MQ=255)
             ccACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACttt                                                         <  1:506739/71‑1 (MQ=255)
                cTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtctc                                                      >  1:3926480/1‑71 (MQ=255)
                 tGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCg                                                     >  1:3593843/1‑71 (MQ=255)
                 tGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCg                                                     >  1:4799295/1‑71 (MQ=255)
                  ggCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAAc                                                                      >  1:2219952/1‑53 (MQ=255)
                  ggCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGc                                                    >  1:2411976/1‑71 (MQ=255)
                   gctgccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGcc                                                   >  1:697326/4‑71 (MQ=255)
                   gctgccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGcc                                                   >  1:786951/4‑71 (MQ=255)
                    ctgccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGcc                                                   <  1:5535532/68‑1 (MQ=255)
                    ccgccgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTgg                                                                   <  1:395542/54‑1 (MQ=255)
                    ccgccgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGccc                                                  <  1:3151299/71‑1 (MQ=255)
                       ccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGCCCa                                                 <  1:526871/69‑1 (MQ=255)
                        cgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGCCCATCg                                              <  1:5037709/71‑1 (MQ=255)
                         gccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAAc                                                                      >  1:5606833/1‑46 (MQ=255)
                          ccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGc                                            >  1:433689/1‑71 (MQ=255)
                             tttCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCt                                         >  1:5273455/1‑71 (MQ=255)
                              ttCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTg                                        >  1:5572796/1‑71 (MQ=255)
                               tCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCAtt                                                               <  1:5260805/47‑1 (MQ=255)
                               tCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtct                                                       >  1:5192297/1‑55 (MQ=255)
                               tCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtc                                                        >  1:3790962/1‑54 (MQ=255)
                                  aTAAACGCGGTTTTGACATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAAc                                    >  1:3663530/1‑71 (MQ=255)
                                  aTAAACGCGGTTTTGACATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAAc                                    >  1:3670230/1‑71 (MQ=255)
                                   tAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAg                                                >  1:3800223/1‑58 (MQ=255)
                                     aaCGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCa                                 <  1:4018471/71‑1 (MQ=255)
                                       cgcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACt                                                           >  1:4705379/1‑43 (MQ=255)
                                       cgcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACt                                                           >  1:4415560/1‑43 (MQ=255)
                                        gcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtct                                                       >  1:1380603/1‑46 (MQ=255)
                                        gcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtct                                                       >  1:922753/1‑46 (MQ=255)
                                         cgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCg                             <  1:2474521/71‑1 (MQ=255)
                                             tttGCCATCGCCGGAGATCCCCCAACTGGCATTCACttt                                                         <  1:2655758/39‑1 (MQ=255)
                                              ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAg                                                >  1:976787/1‑47 (MQ=255)
                                              ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGcc                                           >  1:4816747/1‑52 (MQ=255)
                                              ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGa                          <  1:4347880/69‑1 (MQ=255)
                                              ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGa                          <  1:660612/69‑1 (MQ=255)
                                              ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGAc                         <  1:1676490/70‑1 (MQ=255)
                                              ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGAc                         <  1:3019747/70‑1 (MQ=255)
                                              ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACg                        >  1:3011316/1‑71 (MQ=255)
                                              ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTATCGCCCAGCGGCCCTGTAACCCAGGCGTGa                          <  1:5476708/69‑1 (MQ=255)
                                              ttGCCAGCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACg                        >  1:737594/1‑71 (MQ=255)
                                               tGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGt                       >  1:5274956/1‑71 (MQ=255)
                                               tGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGt                       >  1:1211212/1‑71 (MQ=255)
                                                gCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACg                        >  1:592681/1‑69 (MQ=255)
                                                gCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGtt                      >  1:4407448/1‑71 (MQ=255)
                                                    tCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCg                             <  1:102826/60‑1 (MQ=255)
                                                      gCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTaa                                     >  1:2216563/1‑50 (MQ=255)
                                                         ggAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCt             <  1:4734987/71‑1 (MQ=255)
                                                         ggAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCt             <  1:1456238/71‑1 (MQ=255)
                                                          gagaTCCCCCAACTGGCATTCACTTTCTCGCCCATCGGCCCTGt                                       <  1:5020226/44‑1 (MQ=255)
                                                          gagaTCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTg            >  1:3797718/1‑71 (MQ=255)
                                                            gaTCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGa                          >  1:2952483/1‑55 (MQ=255)
                                                             aTCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGt         <  1:1443069/71‑1 (MQ=255)
                                                                 cccAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGt                            <  1:851536/48‑1 (MQ=255)
                                                                 cccAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGt                            <  1:4788619/48‑1 (MQ=255)
                                                                 cccAACTGGCATTCACTTTCTCGCCCAGAGGCCCTGTAACCCAGGCGt                            <  1:3133575/48‑1 (MQ=255)
                                                                   cAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTaa                                     >  1:112187/1‑37 (MQ=255)
                                                                   cAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGc   <  1:3056159/71‑1 (MQ=255)
                                                                    aaCTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCATCCCCCTGGGTGGCTGCa  <  1:5485661/71‑1 (MQ=255)
                                                                     aCTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCa                    >  1:4794662/1‑52 (MQ=255)
                                                                     aCTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCa                    >  1:2803914/1‑52 (MQ=255)
                                                                     aCTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGccc                <  1:4021149/56‑1 (MQ=255)
                                                                      cTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAAccc                                  >  1:2997395/1‑37 (MQ=255)
                                                                      |                                                                    
TACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTTCAGCCCCCTGGGTGGCTGCA  >  Exported/2096561‑2096699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑