Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,128,783	G→A	6.0%	L83L (TTG→TTA)	yhbW →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,128,783	0	G	A	6.0%	304.4 / 5.6	100	L83L (TTG→TTA)	yhbW	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (37/57);  new base A (4/2);  total (41/59)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

GGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCGGGGCGAAT  >  Exported/2128717‑2128851                                                                   |                                                                     ggTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCt                                                                   <  1:2933546/70‑1 (MQ=255) ggTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCt                                                                   <  1:5343502/70‑1 (MQ=255) ggTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCt                                                                   <  1:471531/70‑1 (MQ=255) ggTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCt                                                                   >  1:4004748/1‑70 (MQ=255) ggTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGGCTGGCGGCGTGATGTTGCCTa                                                                  <  1:3758127/71‑1 (MQ=255)  gTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTaa                                                                 >  1:3096037/1‑71 (MQ=255)     ttGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCa                                                              >  1:4240667/1‑71 (MQ=255)      tGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCAc                                                             >  1:1044405/1‑71 (MQ=255)         tCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAAc                                                                <  1:199427/65‑1 (MQ=255)         tCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAAc                                                                <  1:150958/65‑1 (MQ=255)         tCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCa                                                          >  1:3250422/1‑71 (MQ=255)          cGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCAc                                                         >  1:2852645/1‑71 (MQ=255)          cGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCAc                                                         >  1:1542697/1‑71 (MQ=255)           ggCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCAc                                                         <  1:3175396/70‑1 (MQ=255)            gCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCg                                                       >  1:4809070/1‑71 (MQ=255)            gCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCg                                                       >  1:5033214/1‑71 (MQ=255)               aTCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTg                                                    >  1:2881571/1‑71 (MQ=255)               aTCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTg                                                    >  1:2887994/1‑71 (MQ=255)                  tGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGt                                                  >  1:5604197/1‑70 (MQ=255)                   ggcggcGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCa                                                >  1:757079/1‑71 (MQ=255)                   ggcggcGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCa                                                >  1:1855188/1‑71 (MQ=255)                   ggcggcGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCa                                                >  1:3135834/1‑71 (MQ=255)                   ggcggcGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCa                                                >  1:2269511/1‑71 (MQ=255)                    gcggcgAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTTGTc                                                 <  1:2030040/69‑1 (MQ=255)                    gcggcgAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGt                                                  <  1:1831747/68‑1 (MQ=255)                    gcggcaAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTACCAAACCACTCACCGTTGGtg                                                 <  1:2111076/69‑2 (MQ=255)                    gcggcaAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTACCAAACCACTCACCGTTGGtg                                                 <  1:1155315/69‑2 (MQ=255)                     cggcgAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCAtt                                              <  1:3205555/71‑1 (MQ=255)                      ggcgAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTg                                             >  1:898222/1‑71 (MQ=255)                         gAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                          <  1:2432677/71‑1 (MQ=255)                         gAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                          <  1:3612360/71‑1 (MQ=255)                         gAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                          <  1:3690774/71‑1 (MQ=255)                          aaTACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                          >  1:1195383/1‑70 (MQ=255)                          aaTACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGa                                         <  1:5525017/71‑1 (MQ=255)                           aTACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGt                                                  <  1:4987983/61‑1 (MQ=255)                           aTACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGaa                                        >  1:1282772/1‑71 (MQ=255)                            tACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGt                                                  <  1:4974268/60‑1 (MQ=255)                                accacGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGtt                                   >  1:200472/1‑71 (MQ=255)                                    cGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCgg                                >  1:3442286/1‑70 (MQ=255)                                         cATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                          <  1:4171005/55‑1 (MQ=255)                                         cATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                          <  1:2755949/55‑1 (MQ=255)                                         cATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGc                           <  1:5077610/70‑1 (MQ=255)                                         cATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGc                           <  1:2109652/70‑1 (MQ=255)                                         cATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGCTCATTGCAg                                          <  1:2160332/55‑1 (MQ=255)                                          aTCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                         <  1:5173617/71‑1 (MQ=255)                                          aTCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                         <  1:4520205/71‑1 (MQ=255)                                          aTCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                         <  1:5563804/71‑1 (MQ=255)                                          aTCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                         <  1:1416187/71‑1 (MQ=255)                                          aTCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCt                          >  1:725394/1‑70 (MQ=255)                                           tCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTTGTCATTGCAg                                          <  1:820875/53‑1 (MQ=255)                                           tCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                          <  1:4622542/53‑1 (MQ=255)                                           tCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                          <  1:1805496/53‑1 (MQ=255)                                           tCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                          <  1:3186282/53‑1 (MQ=255)                                           tCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                          <  1:2927221/53‑1 (MQ=255)                                             tGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAAc                                       >  1:2481454/1‑54 (MQ=255)                                             tGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                         <  1:4631648/68‑1 (MQ=255)                                             tGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                         <  1:4250587/68‑1 (MQ=255)                                              ggggTCTGGCGGCGTGATGTTGCCTAACCACTCAcc                                                        <  1:1948425/36‑1 (MQ=255)                                              ggggTCTGGCGGCGTGATGTTGCCTAACCACTCAcc                                                        <  1:3989837/36‑1 (MQ=255)                                              ggggTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCt                          <  1:3158381/66‑1 (MQ=255)                                               gggTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTaa                       <  1:4569475/68‑1 (MQ=255)                                               gggTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTaa                       <  1:1666822/68‑1 (MQ=255)                                               gggTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTaa                       <  1:751515/68‑1 (MQ=255)                                               gggTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTaa                       <  1:3154073/68‑1 (MQ=255)                                               gggTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATa                     >  1:4770232/1‑70 (MQ=255)                                                  tCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGtt                                   <  1:2843948/53‑1 (MQ=255)                                                  tCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGtt                                   <  1:526108/53‑1 (MQ=255)                                                  tCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGtt                                   <  1:2297250/53‑1 (MQ=255)                                                  tCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACAct                 <  1:347671/71‑1 (MQ=255)                                                    tGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                          <  1:5201139/44‑1 (MQ=255)                                                    tGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAg                                          <  1:1966137/44‑1 (MQ=255)                                                    tGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCa                              >  1:386594/1‑56 (MQ=255)                                                    tGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACActc                >  1:4533210/1‑70 (MQ=255)                                                    tGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACActc                >  1:1214574/1‑70 (MQ=255)                                                    tGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGCTCGGCACGCtt                         <  1:3654481/61‑1 (MQ=255)                                                      gcggcgTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAg                                     >  1:3809273/1‑47 (MQ=255)                                                      gcggcgTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGtt                                   >  1:5268446/1‑49 (MQ=255)                                                      gcggcgTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGt                                    >  1:5201349/1‑48 (MQ=255)                                                      gcggcgTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACActct               <  1:1853371/69‑1 (MQ=255)                                                      gcggcgTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTa              >  1:3924417/1‑70 (MQ=255)                                                       cggcgTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATc            >  1:2422984/1‑71 (MQ=255)                                                        ggcgTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATcc           <  1:4115837/71‑1 (MQ=255)                                                         gcgTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATcc           <  1:293210/70‑1 (MQ=255)                                                          cgTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCgg         >  1:2086487/1‑71 (MQ=255)                                                           gTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCggg        >  1:302183/1‑71 (MQ=255)                                                            tGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCgggg       >  1:5487209/1‑71 (MQ=255)                                                             gATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCGGGGc      >  1:3159227/1‑71 (MQ=255)                                                             gATGTTACCAAACCACTCACCGTTGGTGATTGCCGAACAGTTCGGCACGCTCAAt                      >  1:1823771/1‑55 (MQ=37)                                                             gATGTTACCAAACCACTCACCGTTGGTGATTGCCGAACAGTTCGGCACGCTCAAt                      >  1:4435032/1‑55 (MQ=37)                                                             gATGTTACCAAACCACTCACCGTTGGTGATTGCCGAACAGTTCGGCACGCTCAAt                      >  1:4944276/1‑55 (MQ=37)                                                             gATGTTACCAAACCACTCACCGTTGGTGATTGCCGAACAGTTCGGCACGCTCAAt                      >  1:4578327/1‑55 (MQ=37)                                                               tGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                         <  1:1590835/50‑1 (MQ=255)                                                               tGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                         <  1:3812922/50‑1 (MQ=255)                                                               tGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                         <  1:2112398/50‑1 (MQ=255)                                                               tGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                         <  1:5153639/50‑1 (MQ=255)                                                               tGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                         <  1:2583454/50‑1 (MQ=255)                                                                 ttGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                         <  1:327661/48‑1 (MQ=255)                                                                 ttGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCtt                         <  1:4672880/48‑1 (MQ=255)                                                                 ttGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATa                     >  1:4714484/1‑52 (MQ=255)                                                                 ttGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCGGGGCGAAt  <  1:1097033/71‑1 (MQ=255)                                                                   |                                                                     GGTATTGATCGGCTATCTGGCGGCGAATACCACCACGCTGCATCTGGGGTCTGGCGGCGTGATGTTGCCTAACCACTCACCGTTGGTCATTGCAGAACAGTTCGGCACGCTTAATACACTCTATCCGGGGCGAAT  >  Exported/2128717‑2128851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑