Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,130,614 A→G 32.7% F97F (TTT→TTC mtr ← tryptophan transporter of high affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,130,6140AG32.7% 140.0 / 97.0 116F97F (TTT→TTCmtrtryptophan transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base A (39/39);  new base G (20/18);  total (59/57)
Fisher's exact test for biased strand distribution p-value = 8.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 131 total aligned reads displayed.

TCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAA  >  Exported/2130546‑2130683
                                                                    |                                                                     
tctGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGcaca                                                                       >  1:4181047/1‑69 (MQ=255)
      aaGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAAt                                                               <  1:1850097/71‑1 (MQ=255)
        ggTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATgg                                                             <  1:635539/71‑1 (MQ=255)
        ggTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATgg                                                             <  1:2725584/71‑1 (MQ=255)
         gtgtGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                                                            <  1:1183978/71‑1 (MQ=255)
          tgtgATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGcaca                                                                       <  1:2728283/59‑1 (MQ=255)
              aTGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAAt                                                               >  1:838448/1‑63 (MQ=255)
              aTGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGc                                                       >  1:3914307/1‑71 (MQ=255)
                 cAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATgg                                                             <  1:3606152/62‑1 (MQ=255)
                  aGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                                                   <  1:5352501/71‑1 (MQ=255)
                  aGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                                                   <  1:4577236/71‑1 (MQ=255)
                  aGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGtt                                                   <  1:2573442/71‑1 (MQ=255)
                   tgaTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACgaa                                                                     >  1:4315429/3‑52 (MQ=255)
                   tgaTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACggt                                                            <  1:4399599/59‑2 (MQ=255)
                   tgaTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGtaa                                                          <  1:2464514/61‑1 (MQ=255)
                   gAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                                                            <  1:2594640/61‑1 (MQ=255)
                   gAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGa                                                            <  1:126007/61‑1 (MQ=255)
                     aTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGat                                                <  1:5186317/71‑2 (MQ=255)
                     aTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGat                                                <  1:1718479/71‑2 (MQ=255)
                     aTCGCACCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGat                                                <  1:4173012/71‑2 (MQ=255)
                      tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGaa                                                           <  1:1585121/59‑1 (MQ=255)
                      tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc                                                >  1:2605545/1‑70 (MQ=255)
                      tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc                                                >  1:2932335/1‑70 (MQ=255)
                      tCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc                                                >  1:4458215/1‑70 (MQ=255)
                       cGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGaaa                                                          >  1:3640919/1‑59 (MQ=255)
                       cGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGaaa                                                          >  1:2291876/1‑59 (MQ=255)
                        gAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCa                                              >  1:1514809/1‑70 (MQ=255)
                        gAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCAc                                             >  1:1087905/1‑71 (MQ=255)
                          aCCATTAGCAGAGATATAGGCATAAGTGATGATATAGAGAACGAAGGCAACggt                                                            <  1:2002172/54‑2 (MQ=255)
                          aCCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGc                                                                  <  1:2124470/48‑1 (MQ=255)
                          aCCATTAGCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACggt                                                            <  1:2605608/54‑2 (MQ=255)
                           ccACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGtt                                          <  1:648042/71‑1 (MQ=255)
                            cACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTc                                         <  1:4121138/71‑1 (MQ=255)
                              cTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt                                           <  1:1466945/67‑1 (MQ=255)
                              cTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt                                           <  1:2089049/67‑1 (MQ=255)
                              cTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTc                                         >  1:2427702/1‑69 (MQ=255)
                                ggCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc                                     >  1:4870493/1‑71 (MQ=255)
                                ggCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGc                                     >  1:4944555/1‑71 (MQ=255)
                                agCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCaa                                                                >  1:5219831/2‑44 (MQ=255)
                                agCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCaa                                                                >  1:1574932/2‑44 (MQ=255)
                                agCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCaa                                                                >  1:2620165/2‑44 (MQ=255)
                                agCAGAGATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGa                                                 <  1:1761793/58‑1 (MQ=255)
                                   agagATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGtaa                                                          >  1:2682723/1‑47 (MQ=255)
                                   agagATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGtaa                                                          >  1:1194759/1‑47 (MQ=255)
                                   agagATATAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGtaa                                                          >  1:3960139/1‑47 (MQ=255)
                                     agatatAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTcc                                        >  1:2351582/3‑63 (MQ=255)
                                     agatatAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTcc                                        >  1:4209174/3‑63 (MQ=255)
                                     agatatAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTcc                                        >  1:2284749/3‑63 (MQ=255)
                                     agatatAGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTcc                                        >  1:5092420/3‑63 (MQ=255)
                                     agatatAGGCATAAGTGAGGATATAAAGAACGAAGGCAACGGTAATACCGCTGATAATGTTcc                                        >  1:5638411/3‑63 (MQ=255)
                                       atatAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCa                                       >  1:2791634/1‑62 (MQ=255)
                                       atatAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCa                                       >  1:3938836/1‑62 (MQ=255)
                                       atatAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCa                                       >  1:1084562/1‑62 (MQ=255)
                                       atatAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGcc                              >  1:1800618/1‑71 (MQ=255)
                                         ataGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGccc                             <  1:3340983/70‑1 (MQ=255)
                                         ataGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGcc                              <  1:5230412/69‑1 (MQ=255)
                                          taGGCATAAGTGAGGATATAGAGAACGAAGGCAACggt                                                            <  1:1024268/38‑2 (MQ=16)
                                          taGGCATAAGTGAGGATATAGAGAACGAAGGCAACggt                                                            <  1:3971318/38‑2 (MQ=16)
                                          taGGCATAAGTGAGGATATAGAGAACGAAGGCAACggt                                                            <  1:503752/38‑2 (MQ=16)
                                          taGGCATAAGTGAGGATATAGAGAACGAAGGCAACggt                                                            <  1:3792709/38‑2 (MQ=16)
                                          taGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGat                                                <  1:652054/50‑2 (MQ=255)
                                          taGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGat                                                <  1:1651353/50‑2 (MQ=255)
                                          taGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTcc                                        >  1:1468136/1‑58 (MQ=255)
                                          taGGCATAAGTGAGGATATAGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTcc                                        >  1:4705894/1‑58 (MQ=255)
                                           aGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGc                                                       >  1:2967980/1‑42 (MQ=255)
                                           aGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGc                                                       >  1:3726080/1‑42 (MQ=255)
                                           aGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGAc                                                <  1:2449244/49‑1 (MQ=255)
                                            ggCATAAGTGAGGATATAGAGAACGAAGGCAACggt                                                            <  1:5040106/36‑2 (MQ=16)
                                            ggCATAAGTGAGGATATAGAGAACGAAGGCAACGGtaa                                                          >  1:1600465/1‑38 (MQ=12)
                                             gCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCa                         >  1:3476297/1‑70 (MQ=255)
                                             gCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCa                         >  1:2994315/1‑70 (MQ=255)
                                                tAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt                                           <  1:2542552/49‑1 (MQ=255)
                                                tAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGt                                           <  1:3513886/49‑1 (MQ=255)
                                                tAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc                     >  1:389185/1‑71 (MQ=255)
                                                  ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTc                                         >  1:4955361/1‑49 (MQ=255)
                                                  ggTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCttt                                 <  1:1562379/57‑1 (MQ=255)
                                                   gTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt                   <  1:4258585/70‑1 (MQ=255)
                                                     cAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCCGCAAATCTTTg                 >  1:2796383/1‑70 (MQ=255)
                                                      aGGATATAGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGt               >  1:3723559/1‑71 (MQ=255)
                                                       ggATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTg              <  1:4305190/71‑1 (MQ=255)
                                                        gATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa             <  1:2813722/71‑1 (MQ=255)
                                                        gATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa             >  1:5392089/1‑71 (MQ=255)
                                                         atatAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGAt            <  1:2089740/71‑1 (MQ=255)
                                                          tataGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATg           >  1:1344534/1‑71 (MQ=255)
                                                            taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTg              >  1:1008746/1‑66 (MQ=255)
                                                            taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTg              <  1:841671/66‑1 (MQ=255)
                                                            taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTg              >  1:2056723/1‑66 (MQ=255)
                                                            taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa             <  1:5029024/67‑1 (MQ=255)
                                                            taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa             <  1:5022463/67‑1 (MQ=255)
                                                            taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa             <  1:3855934/67‑1 (MQ=255)
                                                              gagCACATAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa             <  1:2824126/65‑1 (MQ=255)
                                                              gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCATATCttt                  <  1:11791/60‑1 (MQ=255)
                                                              gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt                  <  1:4053417/60‑1 (MQ=255)
                                                              gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt                  <  1:1267016/60‑1 (MQ=255)
                                                              gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgtg        >  1:836607/1‑70 (MQ=255)
                                                                gCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTgg                >  1:4798413/1‑60 (MQ=255)
                                                                 cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa    <  1:4613688/71‑1 (MQ=255)
                                                                 cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa    <  1:3111314/71‑1 (MQ=255)
                                                                  acaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa    <  1:3226932/70‑1 (MQ=255)
                                                                   caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATg           <  1:3569765/62‑1 (MQ=255)
                                                                    |                                                                     
TCTGCGAAGGTGTGATGCAGAATCGAACCACTGGCAGAAATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAA  >  Exported/2130546‑2130683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: