Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,130,653 G→A 10.6% G84G (GGC→GGT mtr ← tryptophan transporter of high affinity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,130,6530GA10.6% 308.1 / 19.0 113G84G (GGC→GGTmtrtryptophan transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base G (46/55);  new base A (5/7);  total (51/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

ATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAAT  >  Exported/2130585‑2130721
                                                                    |                                                                    
atatAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGcc                                                                    <  1:3590723/71‑1 (MQ=255)
atatAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGcc                                                                    >  1:1800618/1‑71 (MQ=255)
  ataGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGccc                                                                   <  1:3340983/70‑1 (MQ=255)
  ataGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGcc                                                                    <  1:5230412/69‑1 (MQ=255)
   taGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAg                                                                 >  1:17965/1‑71 (MQ=255)
      gCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCa                                                               >  1:3476297/1‑70 (MQ=255)
      gCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCa                                                               >  1:2994315/1‑70 (MQ=255)
         tAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc                                                           >  1:389185/1‑71 (MQ=255)
          aGGTCAGGATATAGAGCACAAAGGCGATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt                                                          >  1:2905092/1‑71 (MQ=255)
            gTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt                                                         <  1:4258585/70‑1 (MQ=255)
              cAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCCGCAAATCTTTg                                                       >  1:2796383/1‑70 (MQ=255)
               aGGATATAGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGt                                                     >  1:3723559/1‑71 (MQ=255)
                 gATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa                                                   >  1:5392089/1‑71 (MQ=255)
                 gATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa                                                   <  1:2813722/71‑1 (MQ=255)
                 gATATAGAGAACGAAGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGa                                                   >  1:3970433/1‑71 (MQ=255)
                   tataGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATg                                                 >  1:1344534/1‑71 (MQ=255)
                     taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTg                                                    >  1:2056723/1‑66 (MQ=255)
                     taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTg                                                    >  1:1008746/1‑66 (MQ=255)
                     taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTg                                                    <  1:841671/66‑1 (MQ=255)
                     taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa                                                   <  1:3855934/67‑1 (MQ=255)
                     taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa                                                   <  1:5022463/67‑1 (MQ=255)
                     taGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa                                                   <  1:5029024/67‑1 (MQ=255)
                       gagCACATAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa                                                   <  1:2824126/65‑1 (MQ=255)
                       gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCATATCttt                                                        <  1:11791/60‑1 (MQ=255)
                       gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt                                                        <  1:4053417/60‑1 (MQ=255)
                       gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt                                                        <  1:1146724/60‑1 (MQ=255)
                       gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCttt                                                        <  1:1267016/60‑1 (MQ=255)
                       gagCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGgtg                                              >  1:836607/1‑70 (MQ=255)
                         gCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTgg                                                      >  1:4798413/1‑60 (MQ=255)
                          cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa                                          <  1:4613688/71‑1 (MQ=255)
                          cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa                                          <  1:3111314/71‑1 (MQ=255)
                          cacaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa                                          <  1:2050087/71‑1 (MQ=255)
                           acgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaa                                          <  1:4131665/67‑1 (MQ=255)
                           acgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaa                                          <  1:5010047/67‑1 (MQ=255)
                           acgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaa                                          <  1:5535236/67‑1 (MQ=255)
                           acaAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa                                          <  1:3226932/70‑1 (MQ=255)
                            cgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaaaa                                        >  1:1372428/3‑71 (MQ=255)
                            cgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaaaa                                        >  1:190574/3‑71 (MQ=255)
                            cgaaGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTaaaa                                        <  1:223424/69‑1 (MQ=255)
                            caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATg                                                 <  1:307056/62‑1 (MQ=255)
                            caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATg                                                 <  1:3569765/62‑1 (MQ=255)
                            caAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaaaa                                        <  1:4942605/71‑1 (MQ=255)
                              aaGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaaaa                                        >  1:2756128/1‑69 (MQ=255)
                               aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt                                                            >  1:3630603/1‑48 (MQ=255)
                               aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAAt                                                            >  1:5668755/1‑48 (MQ=255)
                               aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc                                                           <  1:4152227/49‑1 (MQ=255)
                               aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc                                                           <  1:721850/49‑1 (MQ=255)
                               aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc                                                           <  1:1220998/49‑1 (MQ=255)
                               aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATc                                                           <  1:4633698/49‑1 (MQ=255)
                               aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTc                                     >  1:4489128/1‑71 (MQ=255)
                               aGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTc                                     <  1:2801628/71‑1 (MQ=255)
                               aGGCAATGGAAATGCCGTTAACCACGTTCCAGCCTTTGCCCAGCAAAt                                                            >  1:2848161/1‑48 (MQ=255)
                               aGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGgtgtta                                           >  1:4137638/1‑63 (MQ=255)
                               aGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTAAAACtg                                     <  1:2681888/71‑2 (MQ=255)
                               aGGCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTAAAACtg                                     <  1:3774747/71‑2 (MQ=255)
                                ggCAACGGTAATACCGCTGATAATGTTCCAGGTGTTACCGATTAAATCTTTGGTGATGGTGTTAAAACtgg                                    <  1:2852568/71‑3 (MQ=255)
                                 gCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTg                                                       >  1:2372887/1‑51 (MQ=255)
                                 gCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa                                                   >  1:4735662/1‑55 (MQ=255)
                                 gCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGa                                                   >  1:3868890/1‑55 (MQ=255)
                                   aaTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt                                                         <  1:3966389/47‑1 (MQ=255)
                                   aaTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt                                                     >  1:4753208/1‑51 (MQ=255)
                                   aaTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt                                                     >  1:5610506/1‑51 (MQ=255)
                                   aaTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt                                                     >  1:5439009/1‑51 (MQ=255)
                                   aaTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt                                                     >  1:940966/1‑51 (MQ=255)
                                    aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaaa                                                             >  1:5349269/1‑42 (MQ=255)
                                    aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCaaa                                                             >  1:1236361/1‑42 (MQ=255)
                                    aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt                                                          <  1:3890396/45‑1 (MQ=255)
                                    aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCt                                                          <  1:4840014/45‑1 (MQ=255)
                                    aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTgg                                                      <  1:1941406/49‑1 (MQ=255)
                                    aTGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTgg                                                      <  1:1938430/49‑1 (MQ=255)
                                     tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCtt                                                         >  1:644446/1‑45 (MQ=255)
                                     tGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGt                                                     <  1:1357031/49‑1 (MQ=255)
                                      ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGc                                                                >  1:3105797/1‑37 (MQ=255)
                                      ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGc                                                                >  1:883130/1‑37 (MQ=255)
                                      ggAAATGCCGTTGACCACGTTCCAGCCTTTGCCAAGCAAATCttt                                                        >  1:1344116/1‑45 (MQ=255)
                                        aaaTGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGAtt                            >  1:2521635/1‑71 (MQ=255)
                                        aaaTGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGAtt                            <  1:4854646/71‑1 (MQ=255)
                                         aaTGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCg                                    <  1:2759106/62‑1 (MQ=255)
                                           tGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa                                          <  1:1296079/54‑1 (MQ=255)
                                           tGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTc                           <  1:2190586/69‑1 (MQ=255)
                                           tGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTc                           <  1:1520045/69‑1 (MQ=255)
                                            gCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTg                         >  1:4191754/1‑70 (MQ=255)
                                             ccGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGt                        >  1:5087710/1‑70 (MQ=255)
                                              cGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCaa                                          <  1:268737/51‑1 (MQ=255)
                                               gTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc                                <  1:2726522/60‑1 (MQ=255)
                                               gTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAAcc                                <  1:3655996/60‑1 (MQ=255)
                                                  gACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTc                   <  1:126705/70‑1 (MQ=255)
                                                     cACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGt               >  1:869825/1‑71 (MQ=255)
                                                       cGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGt               >  1:2991543/1‑69 (MQ=255)
                                                       cGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGt               >  1:1774955/1‑69 (MQ=255)
                                                       cGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTa             <  1:1545702/71‑1 (MQ=255)
                                                       cGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTa             <  1:3566885/71‑1 (MQ=255)
                                                               ccTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTc                   >  1:3424217/1‑57 (MQ=255)
                                                               ccTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCa                  >  1:2062936/1‑58 (MQ=255)
                                                               ccTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCa                  >  1:4726282/1‑58 (MQ=255)
                                                               ccTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCa                  >  1:3231600/1‑58 (MQ=255)
                                                                 tttGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTg                         <  1:2077361/49‑1 (MQ=255)
                                                                  ttGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAat  >  1:5413748/1‑71 (MQ=255)
                                                                   tGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAat  <  1:5644314/70‑1 (MQ=255)
                                                                   tGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAat  <  1:2934140/70‑1 (MQ=255)
                                                                    |                                                                    
ATATAGGCATAGGTCAGGATATAGAGCACAAAGGCAATGGAAATGCCGTTGACCACGTTCCAGCCTTTGCCCAGCAAATCTTTGGTGATGGTGTCAAAACTCGAACCGATTCTGTAATTCAGGTTAGCTTCCAGAAT  >  Exported/2130585‑2130721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: