Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,155,530 T→C 14.7% intergenic (+41/+145) dacB → / ← obgE D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,155,5300TC14.7% 73.1 / 6.2 34intergenic (+41/+145)dacB/obgED‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair
Reads supporting (aligned to +/- strand):  ref base T (12/17);  new base C (2/3);  total (14/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

AAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCA  >  Exported/2155466‑2155597
                                                                |                                                                   
aaaGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACa                                                                >  1:2133692/1‑70 (MQ=255)
         tATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCt                                                       <  1:2648301/70‑1 (MQ=255)
                          aaaaaGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcg                                     >  1:2760005/1‑71 (MQ=255)
                           aaaaGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcgg                                    >  1:146625/1‑71 (MQ=255)
                            aaaGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATgcggc                                   >  1:2048622/1‑71 (MQ=255)
                               gAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGt                                 >  1:837092/1‑70 (MQ=255)
                               gAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                <  1:3823536/71‑1 (MQ=255)
                                       ggCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCt                         >  1:4772423/1‑70 (MQ=255)
                                          acaTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGca                                            >  1:4035108/1‑47 (MQ=255)
                                          acaTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc                     >  1:3364822/1‑71 (MQ=255)
                                          acaTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATc                     >  1:4800180/1‑71 (MQ=255)
                                           caTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGtt                                                  <  1:3530875/41‑1 (MQ=255)
                                           caTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGtt                                                  <  1:3065056/41‑1 (MQ=255)
                                           caTGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                <  1:1301565/59‑1 (MQ=255)
                                             tGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                <  1:1927413/57‑1 (MQ=255)
                                             tGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                <  1:2921788/57‑1 (MQ=255)
                                             tGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa                                <  1:2977235/57‑1 (MQ=255)
                                               gCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGc                 <  1:4255506/70‑1 (MQ=255)
                                                   cggCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc             >  1:3673691/2‑70 (MQ=11)
                                                    ggCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaa           <  1:1553601/71‑1 (MQ=11)
                                                    ggCTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa            >  1:3050819/1‑70 (MQ=11)
                                                    ggCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa            >  1:5520019/1‑70 (MQ=255)
                                                      cTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa         <  1:3245084/71‑1 (MQ=11)
                                                      cTTCAGATTGCTGACAAAGTGCGCGTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa         <  1:3841057/71‑1 (MQ=11)
                                                      cTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa         <  1:3285390/71‑1 (MQ=255)
                                                       tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc                                      <  1:5139261/39‑1 (MQ=14)
                                                       tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc                                      <  1:255958/39‑1 (MQ=14)
                                                       tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc                                      <  1:4175532/39‑1 (MQ=14)
                                                       tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCt                         <  1:3603031/52‑1 (MQ=11)
                                                       tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaaa         <  1:1356754/68‑1 (MQ=11)
                                                       tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACaaaa         <  1:4236095/68‑1 (MQ=11)
                                                        acAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATgc                                      <  1:5145935/39‑1 (MQ=14)
                                                         cAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCg      >  1:4382492/1‑71 (MQ=255)
                                                          aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa                              >  1:1573383/1‑46 (MQ=255)
                                                            attgttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt                                <  1:5410303/42‑2 (MQ=255)
                                                            attgttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGtt                                <  1:864957/42‑2 (MQ=255)
                                                            attgttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTg    >  1:474442/1‑70 (MQ=255)
                                                            attgttGACAAAGTGCGCTTTGTTTATGCCGGATGCAGCGtt                                <  1:1713143/42‑2 (MQ=11)
                                                              tgttGACAACGTACGCATTGTTCATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAACAGTGCa  >  1:534654/3‑70 (MQ=11)
                                                              tgttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTa                       >  1:4728969/3‑49 (MQ=11)
                                                              tgttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACAAAATCGTGc   >  1:339141/3‑69 (MQ=255)
                                                                ttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc             <  1:2908284/57‑1 (MQ=40)
                                                                |                                                                   
AAAGATATTTATCAGAACAATTAGTCAAAAAGAAACCCCGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCA  >  Exported/2155466‑2155597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: