| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 311,691 | T→C | 15.4% | T394T (ACA→ACG) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 311,691 | 0 | T | C | 15.4% | 317.8 / 38.2 | 123 | T394T (ACA→ACG) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (52/52); new base C (8/11); total (60/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 124 total aligned reads displayed.
GCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTAC > Exported/311621‑311759 | tCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGt > 1:4643062/1‑71 (MQ=255) cGTTTTCTACCACAACGATGGCGTCATCCCCCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGc > 1:2161141/1‑71 (MQ=255) ttCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCgtgt > 1:5433983/1‑71 (MQ=255) ttCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCgtg < 1:4225786/70‑1 (MQ=255) tCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCgtgt < 1:3700339/70‑1 (MQ=255) caccaccACTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGttga > 1:4149736/2‑69 (MQ=9) ccaccaCTATCGCATCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGAtg < 1:477367/71‑2 (MQ=2) ccACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATc > 1:1276196/1‑71 (MQ=255) acaACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCga < 1:2423164/71‑1 (MQ=255) tGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa < 1:1071670/71‑1 (MQ=255) ggCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa > 1:958283/1‑70 (MQ=255) ggCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa > 1:2725324/1‑70 (MQ=255) gcatCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCaa > 1:1607906/4‑70 (MQ=9) gCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa > 1:3622721/1‑69 (MQ=255) gTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAgg > 1:5049028/1‑71 (MQ=255) gTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAgg < 1:2435204/71‑1 (MQ=255) gTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAgg > 1:5396240/1‑71 (MQ=255) tCATCGACGAGCAGCCCGATGGCAAGCACCATCCCGAACATCGt < 1:1496414/44‑1 (MQ=9) tCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggc < 1:4854868/71‑1 (MQ=255) tCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggc < 1:1420939/71‑1 (MQ=255) aTCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcgg > 1:539611/1‑71 (MQ=255) tCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCaa < 1:2642586/64‑1 (MQ=255) tCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCaa < 1:5550556/64‑1 (MQ=255) tCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcggc < 1:5510265/71‑1 (MQ=255) tCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcgg > 1:4525407/1‑70 (MQ=255) tCCACCAACAGGCAGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCaa < 1:4851666/64‑1 (MQ=255) ccaccaACAGGCCGATGGCGAGCACCATCCCGAACATTg < 1:1978624/39‑1 (MQ=255) caccaACAGGCCGATGGCGAGCACCATCCCGAACATTGtt > 1:4206916/1‑40 (MQ=255) caccaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTGTCGAGAAGCCAAAg > 1:4383075/1‑64 (MQ=255) caccaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTAt > 1:2497605/1‑51 (MQ=255) caccaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCga > 1:2975313/1‑54 (MQ=255) caccaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcggc > 1:65230/1‑69 (MQ=255) accaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAg < 1:3755918/71‑1 (MQ=255) accaACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAg < 1:2964428/71‑1 (MQ=255) ccaACAGGCCGATGGCGAGCACCATCCCGAACATTg < 1:3975930/36‑1 (MQ=255) ccaACAGGCCGATGGCGAGCACCATCCCGAACATTg < 1:5381640/36‑1 (MQ=255) gagcAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAagc < 1:600496/60‑1 (MQ=9) gagcAGCCCGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGa < 1:3877372/68‑1 (MQ=12) caACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTAt < 1:540908/48‑1 (MQ=255) caACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTAt < 1:4071180/48‑1 (MQ=255) caACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTAt < 1:5616912/48‑1 (MQ=255) caACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCga < 1:510460/51‑1 (MQ=255) caACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa > 1:2970675/1‑71 (MQ=255) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGc > 1:2718452/1‑55 (MQ=255) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCaaa > 1:497563/1‑59 (MQ=255) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCaaa > 1:793641/1‑59 (MQ=255) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCaaa > 1:1893672/1‑59 (MQ=255) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa > 1:560383/1‑57 (MQ=255) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCa > 1:5318445/1‑57 (MQ=255) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGAc > 1:5483622/1‑71 (MQ=255) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGAc > 1:3244469/1‑71 (MQ=255) aaCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGAc > 1:4240022/1‑71 (MQ=255) gcAGCCCGATGGCAAGCACCATCCCGAACATCGTTAgtgt < 1:161080/39‑1 (MQ=18) aCAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAg < 1:5028315/67‑1 (MQ=255) aGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGc < 1:841289/71‑1 (MQ=255) ggCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa < 1:572928/71‑1 (MQ=255) ggCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa < 1:4191323/71‑1 (MQ=255) cccGATGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCaa < 1:1437865/70‑1 (MQ=16) ccGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcggc < 1:3557547/59‑1 (MQ=255) cGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAg < 1:1094316/71‑1 (MQ=255) gATGGCTAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGcc < 1:3399714/48‑1 (MQ=255) gATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGcc < 1:3037034/48‑1 (MQ=255) gATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGcc < 1:1626237/48‑1 (MQ=255) aTGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcggc < 1:4838550/56‑1 (MQ=255) aTGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcggc < 1:219989/56‑1 (MQ=255) aTGGCAAGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGt < 1:1344227/71‑1 (MQ=12) tGGCGAGCACCATCCCTAACATTGTTATCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa < 1:4981848/65‑1 (MQ=255) tGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCaa < 1:1394458/48‑1 (MQ=255) ggCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgaga > 1:37014/1‑41 (MQ=255) ggCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCgaga > 1:4550352/1‑41 (MQ=255) ggCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaaa > 1:5263517/1‑66 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc < 1:3276581/71‑1 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc < 1:1676517/71‑1 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc < 1:1865527/71‑1 (MQ=255) gCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTccc < 1:4340837/71‑1 (MQ=255) aaGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTccct > 1:85032/2‑69 (MQ=12) aGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCaa < 1:3260133/43‑1 (MQ=255) aGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAg > 1:2721745/1‑71 (MQ=255) aGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGat > 1:4923939/1‑55 (MQ=12) aGCACCATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTccctaa > 1:1227868/1‑68 (MQ=12) cACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTcc > 1:4627673/1‑65 (MQ=255) cACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGc > 1:1881803/1‑70 (MQ=255) aCCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa > 1:785974/1‑52 (MQ=255) aCCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa > 1:68116/1‑52 (MQ=255) ccATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCa > 1:2161714/1‑69 (MQ=255) cATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGc < 1:1733292/67‑1 (MQ=255) cATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTc > 1:128890/1‑61 (MQ=12) cATCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTCCCTAACaaca > 1:4166017/1‑69 (MQ=12) tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAggcgg > 1:3876650/1‑42 (MQ=255) tCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCaa < 1:4952290/54‑1 (MQ=255) tCCCGAACATCGTTAGTGTGTTGATGGAGTAACCAAAAGCGGCGAGGATGGCAAACGTCCCTAACaaca > 1:91188/1‑67 (MQ=12) ccGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGc < 1:4266106/63‑1 (MQ=255) aaCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGc < 1:2224202/60‑1 (MQ=255) aCATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCgg > 1:4653480/1‑70 (MQ=255) cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa > 1:3814848/1‑41 (MQ=255) cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGa > 1:2130522/1‑41 (MQ=255) cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCa < 1:1352518/46‑1 (MQ=255) cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCg > 1:1447142/1‑68 (MQ=255) cATTGTTAGCGTGTTTATCGAGAAGCCAAAGGAGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCg > 1:1376820/1‑68 (MQ=255) aTTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTAc < 1:4654934/71‑1 (MQ=255) | GCTCAACGTTTTCTACCACAACGATGGCGTCATCCACCAACAGGCCGATGGCGAGCACCATCCCGAACATTGTTAGCGTGTTTATCGAGAAGCCAAAGGCGGCAAGGACGGCAAAGGTCCCGAGCAATACCACCGGTAC > Exported/311621‑311759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |