Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,220,218 T→C 35.0% intergenic (+478/‑500) yhdH → / → accB predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,220,2180TC35.0% 97.8 / 87.5 97intergenic (+478/‑500)yhdH/accBpredicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit
Reads supporting (aligned to +/- strand):  ref base T (32/31);  new base C (12/22);  total (44/53)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

Only 100 of 109 total aligned reads displayed.

ATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACC  >  Exported/2220148‑2220286
                                                                      |                                                                    
aTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt                                                                      <  1:3386335/71‑1 (MQ=255)
aTTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt                                                                      <  1:5607574/71‑1 (MQ=255)
 ttGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACattatt                                                                      >  1:2122867/1‑70 (MQ=255)
 ttGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTAttt                                                                     <  1:2925540/71‑1 (MQ=255)
       aaTCCCCAGCAACCGCTGCGTAATGTCGTGGATCTTGTCGCGTTCCTGGCATCCCTACATTTTTTGTgg                                                                 >  1:4540535/1‑69 (MQ=255)
        aTCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTc                                                               <  1:1818535/70‑1 (MQ=255)
           cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg                                                           <  1:2005162/71‑1 (MQ=255)
           cccAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg                                                           <  1:1736173/71‑1 (MQ=255)
            ctggCAACAGTTGCATAATTTCGTCTATCTTGTGGCGATTCTGGCCCCAATACATTATCTGTGGGCCGTGa                                                          <  1:4910396/68‑1 (MQ=255)
            ctggCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCcgtg                                                           >  1:4042929/4‑70 (MQ=255)
             cAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg                                                           >  1:3741447/1‑69 (MQ=255)
              ggCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCcgtg                                                           >  1:3262699/2‑68 (MQ=255)
               gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGt                                                                >  1:3018610/1‑62 (MQ=255)
               gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGt                                                                >  1:673363/1‑62 (MQ=255)
               gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGt                                                                >  1:5648965/1‑62 (MQ=255)
               gCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGa                                                       <  1:1257394/71‑1 (MQ=255)
               gCAACAGTTGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCcgtg                                                           >  1:1397027/1‑67 (MQ=255)
                 aaCCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGAtt                                                     <  1:5545810/71‑1 (MQ=255)
                    cGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtc                                                  >  1:2923897/1‑71 (MQ=255)
                     gCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtct                                                 >  1:4713829/1‑71 (MQ=255)
                      ttGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTAtttc                                                <  1:431565/70‑1 (MQ=255)
                      ttGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTAtttc                                                <  1:2915009/70‑1 (MQ=255)
                      cTGCGTAATGTCGTCTATCTTGTCGCGCTCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtct                                                 >  1:4510203/1‑70 (MQ=255)
                       tGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCtgtg                                                           <  1:898184/59‑1 (MQ=255)
                       tGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtctc                                                <  1:1517157/70‑1 (MQ=255)
                       tGCATAATTTCGTCTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCa                                               >  1:5541425/1‑71 (MQ=255)
                         cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCa                                               <  1:1943736/69‑1 (MQ=255)
                         cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCa                                               <  1:1744959/69‑1 (MQ=255)
                         cGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCAt                                              >  1:5020110/1‑70 (MQ=255)
                          gTAATGTCGTCTCTCTTGTCGCGATCCTGGCATCCCTACATTATTTGTTGTCTGTGAAGATTATCTCATTg                                            <  1:1870458/71‑1 (MQ=255)
                          gTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTtgtg                                                                  >  1:310254/1‑49 (MQ=255)
                          gTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTg                                            <  1:3085671/71‑1 (MQ=255)
                            aaTGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTg                                            >  1:917296/1‑69 (MQ=255)
                              tttcgtcTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGaa                                                         <  1:1426304/52‑1 (MQ=255)
                              tttcgtcTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGaa                                                         <  1:1332719/52‑1 (MQ=255)
                              tttcgtcTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGa                                                       >  1:1970645/3‑56 (MQ=255)
                              tttcgtcTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGa                                                       >  1:396614/3‑56 (MQ=255)
                              tttcgtcTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGa                                                       >  1:3718100/3‑56 (MQ=255)
                                tcgtcTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGa                                                       >  1:1127986/1‑54 (MQ=255)
                                tcgtcTATCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGccg                                      >  1:2110651/1‑70 (MQ=255)
                                tcgtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGccc                                      >  1:3997921/1‑71 (MQ=255)
                                  gtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGa                                                       >  1:2942814/1‑52 (MQ=255)
                                  gtcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGa                                                       >  1:1761596/1‑52 (MQ=255)
                                   tcTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTc                                   <  1:4592050/71‑1 (MQ=255)
                                     tATCTTGTCGCGATTCTGGCCCCAATACATTATCtg                                                                    <  1:1120337/36‑1 (MQ=255)
                                     tATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtgt                                                 <  1:2807697/55‑3 (MQ=255)
                                     tATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtct                                                 <  1:1077216/55‑1 (MQ=255)
                                     tATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCCCAt                                 >  1:5414755/1‑71 (MQ=255)
                                      aTCTTGTGGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTAttt                                                 <  1:3900014/54‑3 (MQ=255)
                                       tCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGaa                                                         >  1:3616423/1‑45 (MQ=255)
                                       tCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCa                                               <  1:1933036/55‑1 (MQ=255)
                                       tCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCa                                               <  1:1778168/55‑1 (MQ=255)
                                       tCTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATc                                <  1:3981274/70‑1 (MQ=255)
                                       tCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGa                                                          <  1:5494708/44‑1 (MQ=255)
                                       tCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCa                                               >  1:675890/1‑55 (MQ=255)
                                       tCTTGTCGCGATCCGGGCATCCCTACATTATTTGTGGTCTGTGa                                                          <  1:5676670/44‑1 (MQ=255)
                                        cTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGAt                                                      <  1:5350850/47‑1 (MQ=255)
                                        cTTGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGAt                                                      <  1:1378320/47‑1 (MQ=255)
                                         ttGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGAt                                                      <  1:3489104/46‑1 (MQ=255)
                                         ttGTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGAt                                                      <  1:1299084/46‑1 (MQ=255)
                                         ttGTCGCGATCCTGGCATCCCTACATTATTTGTGGt                                                                >  1:303364/1‑36 (MQ=255)
                                          tGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGccc                                      >  1:4032344/1‑61 (MQ=255)
                                          tGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGccc                                      >  1:5464369/1‑61 (MQ=255)
                                           gTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCt                               <  1:465123/67‑1 (MQ=255)
                                           gTCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGc                           >  1:1666235/1‑71 (MQ=255)
                                           gTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCtt                              <  1:483433/68‑1 (MQ=255)
                                           gTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCg                            <  1:876015/70‑1 (MQ=255)
                                            tCGCGATTCTGGCCCCAATACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGct                          >  1:610291/1‑70 (MQ=255)
                                            tCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGc                           <  1:5574589/70‑1 (MQ=255)
                                               cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTc                                   <  1:2089726/59‑1 (MQ=255)
                                               cgATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTc                                   <  1:3938216/59‑1 (MQ=255)
                                                 aTCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggct                     <  1:5189383/71‑1 (MQ=255)
                                                     tGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtct                                                 <  1:141270/39‑1 (MQ=255)
                                                     tGGCATCCCTACATTATTTGTGGTCTGTGAAGATTAtct                                                 <  1:968893/39‑1 (MQ=255)
                                                      ggCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAg                         <  1:2581014/62‑1 (MQ=255)
                                                      ggCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggctggcgt                >  1:61763/1‑69 (MQ=255)
                                                      ggCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgctt                >  1:4919699/1‑71 (MQ=255)
                                                      ggCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgctt                >  1:68418/1‑71 (MQ=255)
                                                       gCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCa                                               >  1:598487/1‑39 (MQ=255)
                                                       gCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggctggct                 <  1:3455878/69‑1 (MQ=255)
                                                       gCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGggctggct                 <  1:84931/69‑1 (MQ=255)
                                                         aTCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgctttt              >  1:4336204/1‑70 (MQ=255)
                                                            ccTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagc          >  1:873650/1‑71 (MQ=255)
                                                              tACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGccg                                      <  1:5139796/41‑2 (MQ=16)
                                                              tACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGccg                                      <  1:905693/41‑2 (MQ=16)
                                                              tACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGccg                                      <  1:5179624/41‑2 (MQ=16)
                                                              tACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTc                                   <  1:2956533/44‑1 (MQ=16)
                                                              tACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTc                                   <  1:685174/44‑1 (MQ=16)
                                                              tACATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTc                                   <  1:5512540/44‑1 (MQ=16)
                                                                cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctttt      >  1:4523401/1‑71 (MQ=255)
                                                                cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctttt      >  1:4123580/1‑71 (MQ=255)
                                                                cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctttt      >  1:4992352/1‑71 (MQ=255)
                                                                cATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctt        <  1:1391394/69‑1 (MQ=255)
                                                                cATTATCTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCTACGCTGGGCTGgctgtactgctttt      <  1:3746792/71‑1 (MQ=255)
                                                                 attattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagcttttc     <  1:2441561/71‑1 (MQ=255)
                                                                 attattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagcttttc     <  1:4611400/71‑1 (MQ=255)
                                                                 attattTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGgcttttcagctt        <  1:4298870/68‑1 (MQ=255)
                                                                  ttatcTGTGGTCCGTGAAGATTATTTCACCGCAGCCGCTCATCtac                             <  1:3175017/46‑3 (MQ=16)
                                                                    attTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCa                          >  1:2353023/1‑47 (MQ=255)
                                                                    attTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCAcc  >  1:606583/1‑71 (MQ=255)
                                                                      |                                                                    
ATTGGGTAATCCCCAGCAACCGCTGCGTAATGTCGTCTATCTTGTCGCGATCCTGGCATCCCTACATTATTTGTGGTCTGTGAAGATTATCTCATTGCAGCCCCTCATCTTCGCAGGGCTGGCTTTTCAGCTTTTCACC  >  Exported/2220148‑2220286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: