| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,225,948 | G→C | 69.2% | V129V (GTG→GTC) | dusB → | tRNA‑dihydrouridine synthase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,225,948 | 0 | G | C | 69.2% | 99.7 / 61.3 | 78 | V129V (GTG→GTC) | dusB | tRNA‑dihydrouridine synthase B |
| Reads supporting (aligned to +/- strand): ref base G (8/16); new base C (25/29); total (33/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
TCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAAC > Exported/2225887‑2226011 | tCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATg > 1:1665820/1‑66 (MQ=255)tCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATg > 1:221103/1‑66 (MQ=255) ttCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTc < 1:1925402/71‑1 (MQ=255) aGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCt < 1:2927601/70‑1 (MQ=255) ctctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTAc > 1:4624023/1‑71 (MQ=255) ctctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTAc > 1:1336361/1‑71 (MQ=255) ctctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTAc > 1:5237067/1‑71 (MQ=255) ctctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTAc > 1:3468253/1‑71 (MQ=255) ctTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTAccc > 1:1295283/1‑71 (MQ=255) ttGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGt > 1:1795504/1‑65 (MQ=255) gCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATg > 1:40647/1‑47 (MQ=255) gCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATg > 1:5041518/1‑47 (MQ=255) gCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAg > 1:5185048/1‑50 (MQ=255) gCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAg > 1:629643/1‑50 (MQ=255) gCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAg > 1:5028377/1‑50 (MQ=255) cAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGt > 1:3917017/1‑62 (MQ=255) cAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTg < 1:2424299/69‑1 (MQ=255) cAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTg > 1:5204703/1‑69 (MQ=255) aGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAg < 1:2090741/71‑1 (MQ=255) gTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGaa < 1:5109469/69‑1 (MQ=255) tACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGAt < 1:3056273/71‑1 (MQ=255) tACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGAt < 1:1958653/71‑1 (MQ=255) tACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGAt < 1:124103/71‑1 (MQ=255) tACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGAt < 1:5350044/71‑1 (MQ=255) tACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGAt < 1:4896597/71‑1 (MQ=255) tACACGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGAt < 1:2655143/71‑1 (MQ=255) cccGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGaa > 1:4654059/1‑39 (MQ=255) cccGGATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTc > 1:2845667/1‑71 (MQ=255) cGGATGTCGTTAAATCGATCGTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGc < 1:2644833/71‑1 (MQ=255) cGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGc < 1:4919816/71‑1 (MQ=255) ggATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCa > 1:3008416/1‑71 (MQ=255) gATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGc < 1:2326509/38‑1 (MQ=255) gATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGc < 1:4220195/38‑1 (MQ=255) gATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGc < 1:4403067/38‑1 (MQ=255) gATGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGc < 1:230534/38‑1 (MQ=255) tGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAg > 1:3374263/1‑38 (MQ=255) tGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGaa < 1:858369/60‑1 (MQ=255) tGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGaa < 1:2089286/60‑1 (MQ=255) tGTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCg < 1:3186101/71‑1 (MQ=255) gTCGTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCt < 1:3392760/56‑1 (MQ=255) gTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTg > 1:3284821/1‑71 (MQ=255) gTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTg > 1:3046298/1‑71 (MQ=255) gTTAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTg > 1:3761167/1‑71 (MQ=255) ttAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCAcc < 1:2714541/65‑1 (MQ=255) ttAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCAcc < 1:2482250/65‑1 (MQ=255) ttAAATCGATCCTTACCGAGGTCGTCAATGCAGTg < 1:4924703/35‑1 (MQ=255) ttAAATCGATCCTTACCGAGGTCGTCAATGCAGTg < 1:3240402/35‑1 (MQ=255) ttAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGaa < 1:2021558/55‑1 (MQ=255) ttAAATCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTgg < 1:4939939/71‑1 (MQ=255) aTCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGTCAc > 1:3956597/1‑71 (MQ=255) aTCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTgg < 1:5102724/67‑1 (MQ=255) aTCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTgg < 1:1868592/67‑1 (MQ=255) tCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTg < 1:3455423/48‑1 (MQ=255) tCGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTg < 1:2284747/48‑1 (MQ=255) cGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGaa > 1:243965/1‑49 (MQ=255) cGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGaa > 1:3241311/1‑49 (MQ=255) cGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGaa > 1:431998/1‑49 (MQ=255) cGATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGaa < 1:3178624/49‑1 (MQ=255) gATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCg < 1:3120779/70‑1 (MQ=255) gATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCg < 1:1077812/70‑1 (MQ=255) gATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTa > 1:131723/1‑41 (MQ=255) gATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCgg < 1:2928151/71‑1 (MQ=255) gATCCTTACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCgg < 1:680092/71‑1 (MQ=255) cTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTg < 1:4969543/59‑1 (MQ=255) cTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTg < 1:3456884/59‑1 (MQ=255) cTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTg < 1:5139341/59‑1 (MQ=255) tACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTggg > 1:275314/1‑59 (MQ=255) tACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTa > 1:3221800/1‑35 (MQ=255) tACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACAcc > 1:5344348/1‑71 (MQ=255) tACCGAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACAcc > 1:5681696/1‑71 (MQ=255) aCCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAaca < 1:3341946/68‑1 (MQ=255) aCCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAaca < 1:5170299/68‑1 (MQ=255) cGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTa < 1:5143346/71‑1 (MQ=255) gAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTggg < 1:3464707/55‑1 (MQ=255) gAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTggg < 1:1480544/55‑1 (MQ=255) gAGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTa < 1:750231/70‑1 (MQ=255) aGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAAc > 1:41939/1‑71 (MQ=255) aGGTCGTCAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAAc > 1:2499402/1‑71 (MQ=255) gAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGaa > 1:3704718/1‑55 (MQ=255) gAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGaa > 1:1101639/1‑55 (MQ=255) | TCGCAGGTTCAGCCCTCTTGCAGTACCCGGATGTCGTTAAATCGATCCTTACCGAGGTCGTGAATGCAGTGGACGTTCCTGTTACCCTGAAGATTCGCACCGGCTGGGCACCGGAACACCGTAAC > Exported/2225887‑2226011 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |