| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,294,017 | T→A | 22.3% | intergenic (+44/‑231) | gldA → / → yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,294,017 | 0 | T | A | 22.3% | 95.5 / 22.7 | 49 | intergenic (+44/‑231) | gldA/yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (19/19); new base A (3/8); total (22/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.00e-01 | |||||||||||
AGCGTTTCCTGCAAGAGTGGGAATAACCTACTCCAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTG > Exported/2293948‑2294083 | aGCGTTTCCTGCAAGAGTGGGAATAACCTACTCCAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAAt > 1:3363786/1‑70 (MQ=255) agagTGGGAATAACCTACTCCAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTa > 1:5656924/1‑71 (MQ=255) tGGGAATAACCTACTCCAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCt < 1:3310428/71‑1 (MQ=255) ggAATAACCTACTCCAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCtt < 1:3205349/70‑1 (MQ=255) ggAATAACCTACTCCAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTa < 1:1404335/71‑1 (MQ=255) ggAATAACCTACTCCAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTa < 1:3821979/71‑1 (MQ=255) ggAATAACCTACTCCAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTa > 1:4039048/1‑71 (MQ=255) aaCCTACTCCAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAgg > 1:2207878/1‑71 (MQ=255) aCCTACTCCAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGc > 1:791442/1‑71 (MQ=255) aCTCCAATCTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTAc > 1:5379214/1‑71 (MQ=255) cAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCaa > 1:2816222/1‑71 (MQ=255) aCTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAAtct > 1:1456124/1‑71 (MQ=255) cTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCa < 1:3503152/66‑1 (MQ=255) cTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCa < 1:5360329/66‑1 (MQ=255) cTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCa < 1:147098/66‑1 (MQ=255) ggCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCa > 1:4320852/1‑70 (MQ=255) gCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTa < 1:3322978/47‑1 (MQ=255) ttGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCaa < 1:4947334/68‑1 (MQ=255) ttGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAata > 1:4860091/1‑70 (MQ=255) ttGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAata > 1:1397808/1‑70 (MQ=255) tGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACCCTTATCAg < 1:5208743/48‑1 (MQ=255) gTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAatatat > 1:2165227/1‑71 (MQ=255) gggAGTTTGAAGGCAAAATTGCCTGATGCGCTACGCTTTTCAGGCCt < 1:1375929/47‑1 (MQ=17) gggAGTTTGAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTg < 1:2340155/69‑1 (MQ=255) ggAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTg < 1:650623/68‑1 (MQ=255) aGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAt < 1:3568451/69‑1 (MQ=255) gTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTg > 1:2191214/1‑71 (MQ=255) tttGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAatata < 1:327036/61‑1 (MQ=255) ttGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAAt > 1:3972747/1‑48 (MQ=255) ttGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAAt > 1:5078741/1‑48 (MQ=255) tGAACGCAAAATTGCCTGATGCGCTACGCTTATCa > 1:1166492/1‑35 (MQ=37) tGAACGCAAAATTGCCTGATGCGCTACGCTTATCa > 1:2506994/1‑35 (MQ=37) cGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCtt > 1:3491545/1‑71 (MQ=255) cGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCaa < 1:3392733/51‑1 (MQ=14) cGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtt < 1:2258950/71‑1 (MQ=255) cGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtt < 1:3711802/71‑1 (MQ=255) cGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtt < 1:3338977/71‑1 (MQ=255) cGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtt < 1:4396510/71‑1 (MQ=255) gCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAat > 1:4557979/1‑51 (MQ=14) gCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCttt < 1:4666928/71‑1 (MQ=255) cAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGc > 1:3312632/1‑63 (MQ=255) aaaaTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:5264793‑M2/1‑70 (MQ=255) aaaaATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATctct > 1:1819627/1‑44 (MQ=14) aaaaATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGATTTTGCGTGCTTTTg > 1:5198063‑M2/1‑70 (MQ=255) aaaaATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg < 1:5487399‑M2/71‑2 (MQ=255) aaaaATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg < 1:1393426‑M2/71‑2 (MQ=255) aaaaTGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg < 1:44739‑M2/70‑2 (MQ=255) aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAttt < 1:4161666/59‑1 (MQ=255) aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAttt < 1:4476929/59‑1 (MQ=255) aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTg < 1:5504369/60‑1 (MQ=255) aaTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGa < 1:4782298/54‑1 (MQ=255) | AGCGTTTCCTGCAAGAGTGGGAATAACCTACTCCAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTG > Exported/2293948‑2294083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |