| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,294,050 | A→C | 56.5% | intergenic (+77/‑198) | gldA → / → yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,294,050 | 0 | A | C | 56.5% | 57.3 / 120.2 | 115 | intergenic (+77/‑198) | gldA/yijF | glycerol dehydrogenase, NAD/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (24/26); new base C (30/35); total (54/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
CAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCA > Exported/2293981‑2294117 | cAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCaa > 1:2816222/1‑71 (MQ=255) aCTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAAtct > 1:1456124/1‑71 (MQ=255) cTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCa < 1:147098/66‑1 (MQ=255) cTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCa < 1:3503152/66‑1 (MQ=255) cTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCa < 1:5360329/66‑1 (MQ=255) ggCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCa > 1:4320852/1‑70 (MQ=255) ttGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAata > 1:4860091/1‑70 (MQ=255) ttGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAata > 1:1397808/1‑70 (MQ=255) gggAGTTTGAAAGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTg < 1:2340155/69‑1 (MQ=255) ggAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTg < 1:650623/68‑1 (MQ=255) aGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAt < 1:3568451/69‑1 (MQ=255) gTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTg > 1:2191214/1‑71 (MQ=255) tttGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAatata < 1:327036/61‑1 (MQ=255) ttGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAAt > 1:3972747/1‑48 (MQ=255) ttGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAAt > 1:5078741/1‑48 (MQ=255) cGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCtt > 1:3491545/1‑71 (MQ=255) cGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCaa < 1:3392733/51‑1 (MQ=14) cGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtt < 1:4396510/71‑1 (MQ=255) cGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtt < 1:2258950/71‑1 (MQ=255) cGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtt < 1:3711802/71‑1 (MQ=255) gCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAat > 1:4557979/1‑51 (MQ=14) gCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCttt < 1:4666928/71‑1 (MQ=255) cAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGc > 1:3312632/1‑63 (MQ=255) aaaaTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:5264793‑M2/1‑70 (MQ=255) aaaaATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGATTTTGCGTGCTTTTg > 1:5198063‑M2/1‑70 (MQ=255) aaaaATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg < 1:1393426‑M2/71‑2 (MQ=255) aaaaATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg < 1:5487399‑M2/71‑2 (MQ=255) aaaaTGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg < 1:44739‑M2/70‑2 (MQ=255) aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAttt < 1:4476929/59‑1 (MQ=255) aaaTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAttt < 1:4161666/59‑1 (MQ=255) aaatGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:3981576/4‑69 (MQ=255) aaTTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGa < 1:4782298/54‑1 (MQ=255) aatgcCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta > 1:2619056‑M2/3‑67 (MQ=255) atGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGt > 1:2619201/2‑60 (MQ=255) atGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTCCGt > 1:4345039/2‑60 (MQ=14) tGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGc < 1:889139/71‑1 (MQ=255) tGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGc < 1:557875/71‑1 (MQ=255) tGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgtagg > 1:223170‑M2/1‑61 (MQ=255) tGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGa > 1:1324775/1‑71 (MQ=255) tGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGa > 1:2831294/1‑71 (MQ=255) gATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGa > 1:4901081/1‑70 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAAt < 1:3007375/47‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGAt < 1:4455300/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAAt > 1:3667451/1‑47 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:197334‑M2/1‑59 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGAt > 1:1621580/1‑70 (MQ=255) tGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTgt < 1:3144606‑M2/60‑3 (MQ=255) tGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTgt < 1:2117498‑M2/60‑3 (MQ=255) tGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTgt < 1:5008087‑M2/60‑3 (MQ=255) tGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATaa < 1:4767790/71‑1 (MQ=255) gcgcTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta < 1:4240040‑M2/60‑4 (MQ=255) gcgcTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta < 1:5430285‑M2/60‑4 (MQ=255) gcgcTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta < 1:900149‑M2/60‑4 (MQ=255) gcgcTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta < 1:2471523‑M2/60‑4 (MQ=255) gcgcTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGc > 1:1789694/1‑53 (MQ=255) cgcTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAg < 1:2057525/70‑1 (MQ=255) cTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTg < 1:2732235/45‑1 (MQ=255) cTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtttt < 1:5571167/54‑1 (MQ=255) cTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCtttt < 1:4523524/54‑1 (MQ=255) cTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta < 1:1730447‑M2/57‑4 (MQ=255) cTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta < 1:1753831‑M2/57‑4 (MQ=255) cTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGaa < 1:4871911/41‑1 (MQ=255) cTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCg < 1:1017138/71‑1 (MQ=255) cTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCg < 1:581835/71‑1 (MQ=255) tACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGc < 1:234742/49‑1 (MQ=255) aCGCTTATCAGGCCTACGCCATCTCTGCAATATATTGa > 1:527559/1‑38 (MQ=255) aCGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCg > 1:2907694/1‑45 (MQ=255) aCGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCg > 1:270371/1‑45 (MQ=255) aCGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgt < 1:465315‑M2/54‑3 (MQ=255) aCGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGt > 1:4276195/1‑46 (MQ=255) aCGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGt > 1:1090727/1‑46 (MQ=255) aCGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGt > 1:3674642/1‑46 (MQ=255) cGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGtt < 1:4114046/70‑1 (MQ=255) cTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCAc > 1:3613057/1‑71 (MQ=255) tATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCg < 1:54883/64‑1 (MQ=255) tATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCg < 1:2644355/64‑1 (MQ=255) aTCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTcc > 1:1376865/1‑71 (MQ=255) tCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:4811485‑M2/1‑45 (MQ=255) tCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTg > 1:1119658‑M2/1‑45 (MQ=255) tCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGcag > 1:5440119/1‑51 (MQ=255) tCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGtt < 1:1285377/64‑1 (MQ=255) tCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCtttt < 1:2993628/45‑1 (MQ=255) tCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCtttt < 1:1718412/45‑1 (MQ=255) cAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCg > 1:4720792/1‑70 (MQ=255) aGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:1977081/1‑56 (MQ=17) aGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:592788/1‑56 (MQ=17) aGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCa < 1:3097563/71‑1 (MQ=255) gCCTACGCCATCTCTGCAATATATTGAATTTGCTTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATc < 1:5565589/71‑1 (MQ=14) cTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:4650485/1‑52 (MQ=14) cTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCg > 1:3390699/1‑71 (MQ=11) tACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTgta > 1:5455641‑M2/1‑38 (MQ=255) aCGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGAt < 1:776566/48‑1 (MQ=255) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:2887121/1‑49 (MQ=14) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATaa > 1:827485/1‑49 (MQ=14) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCgg < 1:2120277/69‑1 (MQ=11) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCgg < 1:1258126/69‑1 (MQ=11) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCa > 1:4381388/1‑71 (MQ=11) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCa > 1:2113870/1‑71 (MQ=11) cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCa > 1:3472742/1‑71 (MQ=11) cGCAATCTCTGCAATATAATGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCTTCCgg < 1:4109549/69‑1 (MQ=255) | CAAACTCCCGGCTTGTCCGGGAGTTTGAACGCAAAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCAATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACTCCGCATCCGGCA > Exported/2293981‑2294117 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |