Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 2,303,886 | G→A | 5.1% | intergenic (+59/‑125) | metJ → / → yiiX | DNA‑binding transcriptional repressor, S‑adenosylmethionine‑binding/predicted peptidoglycan peptidase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 2,303,886 | 0 | G | A | 5.1% | 377.1 / 5.2 | 116 | intergenic (+59/‑125) | metJ/yiiX | DNA‑binding transcriptional repressor, S‑adenosylmethionine‑binding/predicted peptidoglycan peptidase |
Reads supporting (aligned to +/- strand): ref base G (56/54); new base A (4/2); total (60/56) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.80e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.86e-01 |
Only 100 of 118 total aligned reads displayed.
GGAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCA > Exported/2303818‑2303954 | ggAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGt > 1:2796021/1‑70 (MQ=255) gAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCa < 1:308941/71‑1 (MQ=255) tAAAGCAAAAAAGACCGGCGCTGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCat < 1:787971/66‑1 (MQ=255) aaGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCa < 1:2753124/70‑1 (MQ=255) aaaaaaGAGCGGCGGGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTa < 1:1439509/71‑1 (MQ=255) aaaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTAtt > 1:560992/1‑71 (MQ=255) aaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTAtt > 1:446652/1‑70 (MQ=255) aaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTg < 1:456896/71‑1 (MQ=255) agagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTg < 1:3538734/69‑1 (MQ=255) agagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTg < 1:2476149/69‑1 (MQ=255) gagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAAt < 1:3732742/71‑1 (MQ=255) gctggAATTGCCTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcg < 1:423301‑M1/69‑33 (MQ=255) gTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCa < 1:3706707/41‑1 (MQ=255) gTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACtt < 1:4268655/71‑1 (MQ=255) tGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAAATTATTGAATTTGCACGAACTTg < 1:197399/71‑1 (MQ=255) ggAATTGCCTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgtt > 1:1703141‑M1/1‑36 (MQ=255) ggAATTGCCTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgtt > 1:4305531‑M1/1‑36 (MQ=255) ggAATTGCCTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggc < 1:2998829‑M1/67‑32 (MQ=255) gAATTGCCTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgttca > 1:2826639‑M1/1‑35 (MQ=255) gAATTGCCTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgtt < 1:2241225‑M1/69‑35 (MQ=255) gAATTGCCTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgtt < 1:1040741‑M1/69‑35 (MQ=255) gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTg < 1:1978944/59‑1 (MQ=255) gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGt < 1:4594440/70‑1 (MQ=255) gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGt < 1:1434517/70‑1 (MQ=255) gAATCGCCTGATGCGCTACGCTTATCAGACCTACGTCATAT‑T‑GCAATTTa > 1:674553/1‑50 (MQ=255) aaTCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAAt < 1:4816554/55‑1 (MQ=255) ttgccTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgttcacgc > 1:5025649‑M1/3‑32 (MQ=255) ttgccTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgttcacgc > 1:1888675‑M1/3‑32 (MQ=255) ttgccTGATGCGCTACGCTTATCAGGCCTACGagttcagt > 1:5567619‑M1/3‑32 (MQ=255) tCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGa < 1:1657780/51‑1 (MQ=255) tCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTACTGAATTTg > 1:327091/1‑56 (MQ=255) tgcCTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgttcacgcc > 1:4258136‑M1/2‑31 (MQ=255) tgcCTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgttcacgcc > 1:4708630‑M1/2‑31 (MQ=255) gCCTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgttcacgccg > 1:5154604‑M1/1‑30 (MQ=255) gCCTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgttcacgccg > 1:4888826‑M1/1‑30 (MQ=255) gCCTGATGCGCTACGCTTATCAGGCCTACGagttcag > 1:332573‑M1/1‑30 (MQ=255) gCCTGATGCGCTACGCTTATCAGGCCTACGagttca > 1:461289‑M1/1‑30 (MQ=255) gCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGc > 1:4704022/1‑69 (MQ=255) cTGATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataagg > 1:1947449‑M1/1‑28 (MQ=255) tGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAAtt < 1:3939240/40‑1 (MQ=255) tGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTg < 1:4051071/45‑1 (MQ=255) gATGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgtt < 1:5394254‑M1/60‑35 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgttcacgccgcatcc > 1:1673904‑M1/1‑25 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgttcacgccgcatcc > 1:3107430‑M1/1‑25 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgttcacgccgcatc > 1:3474405‑M1/1‑25 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTa > 1:4396494/1‑40 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt > 1:3972239/1‑69 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt < 1:5246844/69‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt < 1:157414/69‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt < 1:2440714/69‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGa > 1:3907292/1‑70 (MQ=2) tGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGAt > 1:3803956/1‑70 (MQ=2) tGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGAt > 1:3459869/1‑70 (MQ=2) gcTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCg > 1:3102765/1‑71 (MQ=255) cTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGc < 1:3405734/59‑1 (MQ=255) tACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt > 1:4047786/1‑71 (MQ=255) aCGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgttcacgccgcatccggcata > 1:2012476‑M1/1‑18 (MQ=255) aCGCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgttcacgccgcatccggcata > 1:3355961‑M1/1‑18 (MQ=255) aCGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt > 1:3891404/1‑70 (MQ=255) cGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGtt < 1:3029182/71‑1 (MQ=2) gCTTATCAGGCCTACGagttcagtgctctacgtaggccggataaggcgttcacgccgcatccggcata < 1:271830‑M1/68‑53 (MQ=255) gCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTa > 1:3582468/1‑52 (MQ=255) cTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATa > 1:5691889/1‑60 (MQ=255) ttATCAGGCCTACGagttcagtgctgtgtaggtcggataa > 1:4212871‑M1/1‑14 (MQ=255) ttATCAGGCCTACGagttcagtgctgtgtaggtcggataa > 1:1171725‑M1/1‑14 (MQ=255) ttATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGtt < 1:303607/68‑1 (MQ=2) aTCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCAcgccg > 1:3580256/1‑71 (MQ=255) aTCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCAcgc > 1:3866421/1‑71 (MQ=2) tCAGGCCTACGagttcagtgctgtgtaggtcggataaggcgttcacgccgcatccggc < 1:765018‑M1/58‑48 (MQ=255) tCAGGCCTACGagttcagtgctgtgtaggtcggataaggcgttcacgccgcatccggc < 1:2927832‑M1/58‑48 (MQ=255) cAGGCCTACGagttcagtgctgtgtaggtcggataaggcgttcacgccgcatccggcaataacagccttgc > 1:5031944‑M1/1‑10 (MQ=255) cAGGCCTACGagttcagtgctgtgtaggtcggataaggcgttcacgccgcatccggcaataacagccttgc > 1:1660041‑M1/1‑10 (MQ=255) aGGCCTACGagttcagtgctgtgtaggtcggataaggcgttcac < 1:2827852‑M1/44‑36 (MQ=255) aGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAgg > 1:4349288/1‑47 (MQ=255) aGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCAt < 1:5221789/71‑1 (MQ=255) ggCCTACGagttcagtgctgtgtaggtcggataaggcgttcacgccgcatccggcaataacagccttgcct < 1:3217045‑M1/71‑64 (MQ=255) gCCTACGagttcagtgctgtgtaggtcggataaggcgttcacgccgcatccggcaataacagccttgcctg < 1:3302510‑M1/71‑65 (MQ=255) gCCTACGagttcagtgctgtgtaggtcggataaggcgttcacgccgcatccggcaataacagccttgcctg < 1:270384‑M1/71‑65 (MQ=255) gCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATcc > 1:3514639/1‑71 (MQ=255) gCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATc < 1:2469343/70‑1 (MQ=255) cTACGagttcagtgctgtgtaggtcggataaggcgttcacgccgcatccggc < 1:2745454‑M1/52‑48 (MQ=255) cTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGt > 1:426077/1‑56 (MQ=255) cTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg < 1:5416851/71‑1 (MQ=255) aCGagttcagtgctgtgtaggtcggataaggcgttcacgccgcatccggcaataacagccttgcctgacg > 1:1725065‑M1/1‑3 (MQ=255) aCGagttcagtgctgtgtaggtcggataaggcgttcacgccgcatccggcaataa > 1:4640862‑M1/1‑3 (MQ=255) aCGagttcagtgctgtgtaggtcggataaggcgttcacgccgcatccggcaata > 1:4634188‑M1/1‑3 (MQ=255) aCGagttcagtgctgtgtacgtcggataaggcgttcacgccgcatccggcaataa > 1:1313807‑M1/1‑3 (MQ=255) aCGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGa < 1:2987731/46‑1 (MQ=255) cGagttcagtgctgtgtaggtcggataaggcgttcacgccgcatccggcaataacagccttgcctgacgc < 1:4029648‑M1/70‑69 (MQ=255) cGagttcagtgctgtgtaggtcggataaggcgttcacgccgcatccggcaataacagccctgcctgacgc < 1:137436‑M1/70‑69 (MQ=255) cGagttcagtgctgtgtaggtcggataaggcgttcacgccgcatccggcaataa > 1:5196982‑M1/1‑2 (MQ=255) cGagttcagtgctgtgtaggtcggataaggcgttcacgccgcatccggcaataa > 1:2448353‑M1/1‑2 (MQ=255) cGagttcagtgctgtgtaggtcggataaggcgttcacgccgcatccggcaat < 1:3222217‑M1/52‑51 (MQ=255) cGagttcagtgctgtgtaggtcggataaggcgttcacgccgcatccggcaat < 1:4216311‑M1/52‑51 (MQ=255) cGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa > 1:2546639/1‑48 (MQ=255) cGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa > 1:3843765/1‑48 (MQ=255) cGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa > 1:3740576/1‑70 (MQ=255) cGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa > 1:3871214/1‑70 (MQ=255) gagttcagtgctgtgaggtcggataaggcgttcacgccgcatccggcaataacagccttgcctgacgcaat > 1:2675517‑M1/1‑1 (MQ=255) gTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCa > 1:3675332/1‑55 (MQ=255) | GGAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATAT‑T‑GCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCA > Exported/2303818‑2303954 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |