Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,303,887 T→G 9.7% intergenic (+60/‑124) metJ → / → yiiX DNA‑binding transcriptional repressor, S‑adenosylmethionine‑binding/predicted peptidoglycan peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,303,8870TG 9.7% 161.2 / 10.5 62intergenic (+60/‑124)metJ/yiiXDNA‑binding transcriptional repressor, S‑adenosylmethionine‑binding/predicted peptidoglycan peptidase
Reads supporting (aligned to +/- strand):  ref base T (24/32);  new base G (4/2);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01

GGAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCA  >  Exported/2303818‑2303954
                                                                     |                                                                     
ggAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGt                                                                       >  1:2796021/1‑70 (MQ=255)
 gAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCa                                                                     <  1:308941/71‑1 (MQ=255)
       tAAAGCAAAAAAGACCGGCGCTGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCat                                                                    <  1:787971/66‑1 (MQ=255)
         aaGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCa                                                            <  1:2753124/70‑1 (MQ=255)
           gCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAAtt                                                         >  1:2785279/1‑71 (MQ=255)
             aaaaaaGAGCGGCGGGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTa                                                       <  1:1439509/71‑1 (MQ=255)
               aaaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTAtt                                                     >  1:560992/1‑71 (MQ=255)
                aaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTAtt                                                     >  1:446652/1‑70 (MQ=255)
                aaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTg                                                    <  1:456896/71‑1 (MQ=255)
                  agagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTg                                                    <  1:3538734/69‑1 (MQ=255)
                  agagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTg                                                    <  1:2476149/69‑1 (MQ=255)
                   gagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAAt                                                 <  1:3732742/71‑1 (MQ=255)
                           cgGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTa                                                       <  1:3937039/57‑1 (MQ=255)
                               gTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCa                                                                     <  1:3706707/41‑1 (MQ=255)
                               gTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACtt                                     <  1:4268655/71‑1 (MQ=255)
                                tGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCat                                                                    <  1:182463/41‑1 (MQ=255)
                                tGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAAATTATTGAATTTGCACGAACTTg                                    <  1:197399/71‑1 (MQ=255)
                                  gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTg                                              <  1:1978944/59‑1 (MQ=255)
                                  gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGt                                   <  1:4594440/70‑1 (MQ=255)
                                  gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGt                                   <  1:1434517/70‑1 (MQ=255)
                                  gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGt                                   <  1:843407/70‑1 (MQ=255)
                                  gAATCGCCTGATGCGCTACGCTTATCAGACCTACGTCATATTGCAATTTa                                                       >  1:674553/1‑50 (MQ=255)
                                   aaTCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAAt                                                 <  1:4816554/55‑1 (MQ=255)
                                   aaTCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTg                                    <  1:4278153/68‑1 (MQ=255)
                                     tCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGa                                                   <  1:1657780/51‑1 (MQ=255)
                                     tCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTACTGAATTTg                                              >  1:327091/1‑56 (MQ=255)
                                       gCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGc                               >  1:4704022/1‑69 (MQ=255)
                                          tGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAAtt                                                         <  1:3939240/40‑1 (MQ=255)
                                          tGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTg                                                    <  1:4051071/45‑1 (MQ=255)
                                            aTGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTa                                                       >  1:4396494/1‑40 (MQ=255)
                                            aTGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                          <  1:5246844/69‑1 (MQ=255)
                                            aTGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                          <  1:2440714/69‑1 (MQ=255)
                                            aTGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                          <  1:157414/69‑1 (MQ=255)
                                            aTGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                          >  1:3972239/1‑69 (MQ=255)
                                            aTGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGa                           >  1:3907292/1‑70 (MQ=2)
                                             tGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGAt                          >  1:3459869/1‑70 (MQ=2)
                                             tGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGAt                          >  1:3803956/1‑70 (MQ=2)
                                                gcTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCg                    >  1:3102765/1‑71 (MQ=255)
                                                 cTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGc                               <  1:3405734/59‑1 (MQ=255)
                                                  tACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt                  >  1:5184782/1‑71 (MQ=255)
                                                  tACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt                  >  1:4047786/1‑71 (MQ=255)
                                                   aCGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt                  >  1:3891404/1‑70 (MQ=255)
                                                    cGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGtt                  <  1:3029182/71‑1 (MQ=2)
                                                     gCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTa                                  >  1:3582468/1‑52 (MQ=255)
                                                      cTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATa                         >  1:5691889/1‑60 (MQ=255)
                                                       ttATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGtt                  <  1:303607/68‑1 (MQ=2)
                                                         aTCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCAcgccg           >  1:3580256/1‑71 (MQ=255)
                                                         aTCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCAcgc             >  1:3866421/1‑71 (MQ=2)
                                                            aGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAgg                                >  1:4349288/1‑47 (MQ=255)
                                                            aGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                          <  1:3365622/53‑1 (MQ=255)
                                                            aGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCAt        <  1:5221789/71‑1 (MQ=255)
                                                             ggCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATc       <  1:3792219/71‑1 (MQ=255)
                                                              gCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATcc      >  1:3514639/1‑71 (MQ=255)
                                                              gCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATc       <  1:2469343/70‑1 (MQ=255)
                                                                cTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGt                   >  1:426077/1‑56 (MQ=255)
                                                                cTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg    <  1:5416851/71‑1 (MQ=255)
                                                                  aCGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGa                           <  1:2987731/46‑1 (MQ=255)
                                                                   cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa                        >  1:2546639/1‑48 (MQ=255)
                                                                   cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa                        >  1:3843765/1‑48 (MQ=255)
                                                                   cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa  >  1:4573581/1‑70 (MQ=255)
                                                                   cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa  >  1:3740576/1‑70 (MQ=255)
                                                                   cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa  >  1:3871214/1‑70 (MQ=255)
                                                                    gTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCa                >  1:3675332/1‑55 (MQ=255)
                                                                     tCATATTGCAATTTATTGAATTTGCACGAACTTGTa                                  >  1:2644667/1‑36 (MQ=255)
                                                                     tCATATTGCAATTTATTGAATTTGCACGAACTTGTa                                  >  1:188142/1‑36 (MQ=255)
                                                                     |                                                                     
GGAATACTAAAGCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCA  >  Exported/2303818‑2303954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: