Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,303,898 T→C 9.4% intergenic (+71/‑113) metJ → / → yiiX DNA‑binding transcriptional repressor, S‑adenosylmethionine‑binding/predicted peptidoglycan peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,303,8980TC 9.4% 199.0 / 9.5 77intergenic (+71/‑113)metJ/yiiXDNA‑binding transcriptional repressor, S‑adenosylmethionine‑binding/predicted peptidoglycan peptidase
Reads supporting (aligned to +/- strand):  ref base T (38/31);  new base C (5/2);  total (43/34)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

GCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGAGCA  >  Exported/2303829‑2303967
                                                                       |                                                                     
gCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAAtt                                                                      >  1:2785279/1‑71 (MQ=255)
  aaaaaaGAGCGGCGGGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTa                                                                    <  1:1439509/71‑1 (MQ=255)
    aaaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTAtt                                                                  >  1:560992/1‑71 (MQ=255)
     aaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTAtt                                                                  >  1:446652/1‑70 (MQ=255)
     aaaGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTg                                                                 <  1:456896/71‑1 (MQ=255)
       agagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTg                                                                 <  1:3538734/69‑1 (MQ=255)
       agagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTg                                                                 <  1:2476149/69‑1 (MQ=255)
        gagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAAt                                                              <  1:3732742/71‑1 (MQ=255)
                cgGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTa                                                                    <  1:3937039/57‑1 (MQ=255)
                    gTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACtt                                                  <  1:4268655/71‑1 (MQ=255)
                     tGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAAATTATTGAATTTGCACGAACTTg                                                 <  1:197399/71‑1 (MQ=255)
                       gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTg                                                           <  1:1978944/59‑1 (MQ=255)
                       gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGt                                                <  1:4594440/70‑1 (MQ=255)
                       gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGt                                                <  1:1434517/70‑1 (MQ=255)
                       gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGt                                                <  1:843407/70‑1 (MQ=255)
                       gAATCGCCTGATGCGCTACGCTTATCAGACCTACGTCATATTGCAATTTa                                                                    >  1:674553/1‑50 (MQ=255)
                        aaTCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAAt                                                              <  1:4816554/55‑1 (MQ=255)
                        aaTCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTg                                                 <  1:4278153/68‑1 (MQ=255)
                          tCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGa                                                                <  1:1657780/51‑1 (MQ=255)
                          tCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTACTGAATTTg                                                           >  1:327091/1‑56 (MQ=255)
                            gCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGc                                            >  1:4704022/1‑69 (MQ=255)
                               tGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAAtt                                                                      <  1:3939240/40‑1 (MQ=255)
                               tGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTg                                                                 <  1:4051071/45‑1 (MQ=255)
                                 aTGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTa                                                                    >  1:4396494/1‑40 (MQ=255)
                                 aTGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                                       <  1:157414/69‑1 (MQ=255)
                                 aTGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                                       >  1:3972239/1‑69 (MQ=255)
                                 aTGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                                       <  1:5246844/69‑1 (MQ=255)
                                 aTGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                                       <  1:2440714/69‑1 (MQ=255)
                                 aTGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGa                                        >  1:3907292/1‑70 (MQ=2)
                                  tGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGAt                                       >  1:3803956/1‑70 (MQ=2)
                                  tGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGAt                                       >  1:3459869/1‑70 (MQ=2)
                                     gcTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCg                                 >  1:3102765/1‑71 (MQ=255)
                                      cTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGc                                            <  1:3405734/59‑1 (MQ=255)
                                       tACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt                               >  1:4047786/1‑71 (MQ=255)
                                       tACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt                               >  1:5184782/1‑71 (MQ=255)
                                        aCGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt                               >  1:3891404/1‑70 (MQ=255)
                                         cGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGtt                               <  1:3029182/71‑1 (MQ=2)
                                          gCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTa                                               >  1:3582468/1‑52 (MQ=255)
                                           cTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATa                                      >  1:5691889/1‑60 (MQ=255)
                                            ttATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGtt                               <  1:303607/68‑1 (MQ=2)
                                              aTCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCAcgccg                        >  1:3580256/1‑71 (MQ=255)
                                              aTCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCAcgc                          >  1:3866421/1‑71 (MQ=2)
                                                 aGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAgg                                             >  1:4349288/1‑47 (MQ=255)
                                                 aGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                                       <  1:3365622/53‑1 (MQ=255)
                                                 aGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCAt                     <  1:5221789/71‑1 (MQ=255)
                                                  ggCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATc                    <  1:3792219/71‑1 (MQ=255)
                                                   gCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATcc                   >  1:3514639/1‑71 (MQ=255)
                                                   gCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATc                    <  1:2469343/70‑1 (MQ=255)
                                                     cTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGt                                >  1:426077/1‑56 (MQ=255)
                                                     cTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg                 <  1:5416851/71‑1 (MQ=255)
                                                       aCGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGa                                        <  1:2987731/46‑1 (MQ=255)
                                                        cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa                                     >  1:2546639/1‑48 (MQ=255)
                                                        cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa                                     >  1:3843765/1‑48 (MQ=255)
                                                        cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa               >  1:3871214/1‑70 (MQ=255)
                                                        cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa               >  1:3740576/1‑70 (MQ=255)
                                                        cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa               >  1:4573581/1‑70 (MQ=255)
                                                         gTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCa                             >  1:3675332/1‑55 (MQ=255)
                                                          tCATATTGCAATTTATTGAATTTGCACGAACTTGTa                                               >  1:2644667/1‑36 (MQ=255)
                                                          tCATATTGCAATTTATTGAATTTGCACGAACTTGTa                                               >  1:188142/1‑36 (MQ=255)
                                                            atatTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaac          <  1:4141506/71‑1 (MQ=255)
                                                             tatTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaaca         <  1:856903/71‑1 (MQ=255)
                                                              atTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaacaa        >  1:2532159/1‑71 (MQ=255)
                                                              atTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaacaa        >  1:2453031/1‑71 (MQ=255)
                                                                 tggCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAaaaaa          >  1:4308557/3‑67 (MQ=14)
                                                                 tGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAaacaac       >  1:948933/1‑70 (MQ=14)
                                                                 tGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAaacaac       >  1:3159430/1‑70 (MQ=14)
                                                                 tGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa                                     >  1:1396753/1‑40 (MQ=255)
                                                                 tGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa                                     >  1:1536045/1‑40 (MQ=255)
                                                                 tGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAgg                                   >  1:4843121/1‑42 (MQ=255)
                                                                 tGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAgg                                   >  1:5499850/1‑42 (MQ=255)
                                                                  cgCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATaa                                     <  1:4310830/39‑1 (MQ=9)
                                                                  cgCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATaa                                     <  1:1560332/39‑1 (MQ=9)
                                                                  cgCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATaa                                     <  1:2203729/39‑1 (MQ=9)
                                                                  cgCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATaa                                     >  1:252804/2‑40 (MQ=9)
                                                                  cgCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATaa                                     <  1:2282605/39‑1 (MQ=9)
                                                                  cgCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATa                                      >  1:4462287/2‑39 (MQ=9)
                                                                   gCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaacaac       >  1:127821/1‑69 (MQ=255)
                                                                   gCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGAt                                       >  1:4527265/1‑37 (MQ=9)
                                                                      aTTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaacaa        >  1:4600911/1‑65 (MQ=255)
                                                                       tttATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGcc  >  1:3177189/1‑69 (MQ=11)
                                                                       tttATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGt                                <  1:3047079/40‑1 (MQ=255)
                                                                       tttATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGt                                <  1:2223893/40‑1 (MQ=255)
                                                                       tttATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGt                                <  1:5144503/40‑1 (MQ=255)
                                                                       tttATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGt                                <  1:3885705/40‑1 (MQ=255)
                                                                       |                                                                     
GCAAAAAAGAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGAGCA  >  Exported/2303829‑2303967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: