Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,303,906 A→T 11.4% intergenic (+79/‑105) metJ → / → yiiX DNA‑binding transcriptional repressor, S‑adenosylmethionine‑binding/predicted peptidoglycan peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,303,9060AT11.4% 216.9 / 19.8 88intergenic (+79/‑105)metJ/yiiXDNA‑binding transcriptional repressor, S‑adenosylmethionine‑binding/predicted peptidoglycan peptidase
Reads supporting (aligned to +/- strand):  ref base A (42/36);  new base T (6/4);  total (48/40)
Fisher's exact test for biased strand distribution p-value = 7.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGAGCACGTTGTC  >  Exported/2303837‑2303974
                                                                       |                                                                    
gagCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAAt                                                                     <  1:3732742/71‑1 (MQ=255)
            gTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACtt                                                         <  1:4268655/71‑1 (MQ=255)
             tGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAAATTATTGAATTTGCACGAACTTg                                                        <  1:197399/71‑1 (MQ=255)
               gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTg                                                                  <  1:1978944/59‑1 (MQ=255)
               gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGt                                                       <  1:843407/70‑1 (MQ=255)
               gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGt                                                       <  1:4594440/70‑1 (MQ=255)
               gAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGt                                                       <  1:1434517/70‑1 (MQ=255)
                aaTCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAAt                                                                     <  1:4816554/55‑1 (MQ=255)
                aaTCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTg                                                        <  1:4278153/68‑1 (MQ=255)
                  tCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTACTGAATTTg                                                                  >  1:327091/1‑56 (MQ=255)
                    gCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGc                                                   >  1:4704022/1‑69 (MQ=255)
                         aTGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                                              <  1:157414/69‑1 (MQ=255)
                         aTGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                                              <  1:5246844/69‑1 (MQ=255)
                         aTGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                                              >  1:3972239/1‑69 (MQ=255)
                         aTGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                                              <  1:2440714/69‑1 (MQ=255)
                         aTGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGa                                               >  1:3907292/1‑70 (MQ=2)
                          tGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGAt                                              >  1:3459869/1‑70 (MQ=2)
                          tGCGCTACGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGAt                                              >  1:3803956/1‑70 (MQ=2)
                             gcTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCg                                        >  1:3102765/1‑71 (MQ=255)
                              cTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGc                                                   <  1:3405734/59‑1 (MQ=255)
                               tACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt                                      >  1:4047786/1‑71 (MQ=255)
                               tACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt                                      >  1:5184782/1‑71 (MQ=255)
                                aCGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt                                      >  1:3891404/1‑70 (MQ=255)
                                 cGCTTATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGtt                                      <  1:3029182/71‑1 (MQ=2)
                                  gCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTa                                                      >  1:3582468/1‑52 (MQ=255)
                                   cTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATa                                             >  1:5691889/1‑60 (MQ=255)
                                    ttATCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGtt                                      <  1:303607/68‑1 (MQ=2)
                                      aTCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCAcgccg                               >  1:3580256/1‑71 (MQ=255)
                                      aTCAGGCCTACAGGATATCTGGCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCAcgc                                 >  1:3866421/1‑71 (MQ=2)
                                         aGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAgg                                                    >  1:4349288/1‑47 (MQ=255)
                                         aGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGAt                                              <  1:3365622/53‑1 (MQ=255)
                                         aGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCAt                            <  1:5221789/71‑1 (MQ=255)
                                          ggCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATc                           <  1:3792219/71‑1 (MQ=255)
                                           gCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATcc                          >  1:3514639/1‑71 (MQ=255)
                                           gCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATc                           <  1:2469343/70‑1 (MQ=255)
                                             cTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGt                                       >  1:426077/1‑56 (MQ=255)
                                             cTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg                        <  1:5416851/71‑1 (MQ=255)
                                               aCGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGa                                               <  1:2987731/46‑1 (MQ=255)
                                                cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa                                            >  1:3843765/1‑48 (MQ=255)
                                                cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa                                            >  1:2546639/1‑48 (MQ=255)
                                                cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                      >  1:3740576/1‑70 (MQ=255)
                                                cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                      >  1:4573581/1‑70 (MQ=255)
                                                cGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                      >  1:3871214/1‑70 (MQ=255)
                                                 gTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCa                                    >  1:3675332/1‑55 (MQ=255)
                                                  tCATATTGCAATTTATTGAATTTGCACGAACTTGTa                                                      >  1:2644667/1‑36 (MQ=255)
                                                  tCATATTGCAATTTATTGAATTTGCACGAACTTGTa                                                      >  1:188142/1‑36 (MQ=255)
                                                    atatTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaac                 <  1:4141506/71‑1 (MQ=255)
                                                     tatTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaaca                <  1:856903/71‑1 (MQ=255)
                                                      atTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaacaa               >  1:2532159/1‑71 (MQ=255)
                                                      atTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaacaa               >  1:2453031/1‑71 (MQ=255)
                                                         tggCAACTTATTAAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCACGCCGCATCCGGCAaaaaa                 >  1:4308557/3‑67 (MQ=14)
                                                         tGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAaacaac              >  1:948933/1‑70 (MQ=14)
                                                         tGCAATTTATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAaacaac              >  1:3159430/1‑70 (MQ=14)
                                                         tGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa                                            >  1:1396753/1‑40 (MQ=255)
                                                         tGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATaa                                            >  1:1536045/1‑40 (MQ=255)
                                                         tGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAgg                                          >  1:5499850/1‑42 (MQ=255)
                                                         tGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAgg                                          >  1:4843121/1‑42 (MQ=255)
                                                          cgCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATaa                                            <  1:2203729/39‑1 (MQ=9)
                                                          cgCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATaa                                            <  1:1560332/39‑1 (MQ=9)
                                                          cgCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATaa                                            >  1:252804/2‑40 (MQ=9)
                                                          cgCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATaa                                            <  1:2282605/39‑1 (MQ=9)
                                                          cgCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATaa                                            <  1:4310830/39‑1 (MQ=9)
                                                          cgCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGATa                                             >  1:4462287/2‑39 (MQ=9)
                                                           gCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaacaac              >  1:127821/1‑69 (MQ=255)
                                                           gCAATTTATCGATTTTGCGAGCACTTGTAGGCCGGAt                                              >  1:4527265/1‑37 (MQ=9)
                                                              aTTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAaacaa               >  1:4600911/1‑65 (MQ=255)
                                                               tttATTGATTTTGCAAGACTTTGTGGGTCGGATAAGGCGTTCACGCCGCATCCGGCAAAAACAACGAGcc         >  1:3177189/1‑69 (MQ=11)
                                                               tttATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGt                                       <  1:3885705/40‑1 (MQ=255)
                                                               tttATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGt                                       <  1:2223893/40‑1 (MQ=255)
                                                               tttATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGt                                       <  1:5144503/40‑1 (MQ=255)
                                                               tttATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGt                                       <  1:3047079/40‑1 (MQ=255)
                                                                ttattaAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCACGCCGCa                             <  1:1191464/49‑1 (MQ=14)
                                                                ttattaAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCACGCCGCa                             <  1:3061514/49‑1 (MQ=14)
                                                                ttattaAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCACGCCGCa                             <  1:3138717/49‑1 (MQ=14)
                                                                ttattaAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCACGCCGCa                             <  1:1336786/49‑1 (MQ=14)
                                                                ttATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGAGCACg       >  1:5221284/1‑71 (MQ=255)
                                                                 tATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCa                                    >  1:2428901/1‑41 (MQ=38)
                                                                 tATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCa                                    >  1:2501847/1‑41 (MQ=38)
                                                                 tATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCa                                    >  1:1490483/1‑41 (MQ=38)
                                                                 tATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATaaa                  >  1:4563262/1‑59 (MQ=255)
                                                                 tATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGAGCACGt      >  1:2382162/1‑71 (MQ=255)
                                                                  aTTAAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCa                                    >  1:1024020/1‑40 (MQ=14)
                                                                  aTTAAAATTGCATGAACTTGTAGGACGGATAAGGCGTTCa                                    >  1:5552732/1‑40 (MQ=14)
                                                                   ttGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGAGCACGtt     <  1:1826962/70‑1 (MQ=255)
                                                                   ttGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGAGCACGtt     <  1:3852601/70‑1 (MQ=255)
                                                                     gAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt                                      <  1:4438711/35‑1 (MQ=40)
                                                                     gAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt                                      <  1:85072/35‑1 (MQ=40)
                                                                     gAATTTGCACGAACTTGTAGGCCGGATAAGGCGtt                                      <  1:1782587/35‑1 (MQ=40)
                                                                       aTTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGAGCACGTTGTc  >  1:4089437/1‑69 (MQ=255)
                                                                       |                                                                    
GAGCGGCGCGGAGTGGAATCGCCTGATGCGCTACGCTTATCAGGCCTACGTCATATTGCAATTTATTGAATTTGCACGAACTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATAAACAACGAGCACGTTGTC  >  Exported/2303837‑2303974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: