| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,320,679 | T→C | 7.4% | G94G (GGT→GGC) | tpiA → | triosephosphate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,320,679 | 0 | T | C | 7.4% | 226.6 / 6.2 | 81 | G94G (GGT→GGC) | tpiA | triosephosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base T (43/32); new base C (2/4); total (45/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
GCATTCACCGGTGAAACCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATCGCGAAAAAATTCGCGGTGCT > Exported/2320614‑2320744 | gcATTCACCGGTGAAACCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCActc > 1:96002/1‑71 (MQ=255) aTTCACCGGTGAAACCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTg < 1:43389/71‑1 (MQ=255) aTTCACCGGTGAAACCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTg < 1:2357239/71‑1 (MQ=255) aTTCACCGGTGAAACCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTg < 1:2336015/71‑1 (MQ=255) ttCACCGGTGAAAACTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTg < 1:2858310/70‑1 (MQ=255) cACCGGTGAAACCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAAc > 1:487132/1‑71 (MQ=255) cACCGGTGAAACCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAAc > 1:176647/1‑71 (MQ=255) ggTGAAACCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAAcgtc > 1:5318228/1‑70 (MQ=255) gTGAAACCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGCCACTCTGAAcgtcg > 1:5360637/1‑70 (MQ=255) gTGAAACCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGCCACTCTGAAagtcg > 1:2399295/1‑70 (MQ=255) tGAAACCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAAcgtcgt < 1:1199210/70‑1 (MQ=255) tGAAACCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAAcgtcgt < 1:4033749/70‑1 (MQ=255) tGAAACCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTa < 1:3594492/71‑1 (MQ=255) aaaCCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAAcgtcgt < 1:297026/68‑1 (MQ=255) aaCCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACtt < 1:1924587/71‑1 (MQ=255) aaCCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACtt < 1:4380631/71‑1 (MQ=255) aCCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGaa > 1:936162/1‑60 (MQ=255) aCCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGaa > 1:2272963/1‑60 (MQ=255) aCCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTa > 1:670688/1‑71 (MQ=255) ctgctgCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACca > 1:560664/1‑70 (MQ=255) tgctgcTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGt < 1:3669932/46‑1 (MQ=255) ctgctATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACcaca < 1:2673637/69‑1 (MQ=255) ctgctATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACca > 1:4603996/1‑67 (MQ=255) ctgctATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACca > 1:1330358/1‑67 (MQ=255) ctgctATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACca > 1:616427/1‑67 (MQ=255) ctgctATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACca > 1:1878756/1‑67 (MQ=255) ctgctATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACca > 1:3567700/1‑67 (MQ=255) ctATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAg < 1:2003432/69‑1 (MQ=255) ctATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGa < 1:1756706/70‑1 (MQ=255) tATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTa > 1:2790666/1‑60 (MQ=255) tATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTa > 1:1916056/1‑60 (MQ=255) tGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTa > 1:4394553/1‑54 (MQ=255) tGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATc < 1:4685321/70‑1 (MQ=255) tGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCt < 1:4561810/71‑1 (MQ=255) gCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTAc > 1:4642711/1‑54 (MQ=255) gCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTAc > 1:120006/1‑54 (MQ=255) gCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTAc > 1:2340890/1‑54 (MQ=255) cTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAAt > 1:4768753/1‑67 (MQ=255) cTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAAt > 1:3806328/1‑67 (MQ=255) cTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAAt > 1:3194556/1‑67 (MQ=255) cTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGa > 1:1167190/1‑71 (MQ=255) cTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACAACAAAGAAt > 1:604150/1‑67 (MQ=255) aaaGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACGAAGAATCTGACg < 1:3072953/70‑1 (MQ=255) aaaGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGAc > 1:1627575/1‑69 (MQ=255) aaaGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACg > 1:3061013/1‑70 (MQ=255) gACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGa < 1:455657/59‑1 (MQ=255) aCATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAAt < 1:719756/60‑1 (MQ=255) cATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTAc > 1:4523580/1‑45 (MQ=255) cATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTg < 1:1261325/70‑1 (MQ=255) cATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGa < 1:3474389/71‑1 (MQ=255) cATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTAGTACTTACCACAAAGAATCTGACGAACTg < 1:5044208/70‑1 (MQ=255) aTCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCt > 1:2600684/1‑60 (MQ=255) aTCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCt > 1:805561/1‑60 (MQ=255) aTCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGAt < 1:3751083/71‑1 (MQ=255) aTCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGAt > 1:4641888/1‑71 (MQ=255) tCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATc > 1:159791/1‑71 (MQ=255) ggCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGa > 1:2038194/1‑59 (MQ=255) ggCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGa > 1:1451718/1‑59 (MQ=255) ggCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATcgc > 1:5600729/1‑71 (MQ=255) ggCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATcgc > 1:2122280/1‑71 (MQ=255) gcgcACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATcgcg < 1:2513946/71‑1 (MQ=255) cgcACAGTACATCATCATCGGTCACTCTGAACGTCGTACtt < 1:5181923/41‑1 (MQ=255) gcACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGAGCGCGaa < 1:2433/71‑1 (MQ=255) cacaGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAg > 1:185500/1‑48 (MQ=255) acaGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATCGCGaaaa < 1:4511153/71‑1 (MQ=255) acaGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATCGCGaaaa < 1:5627180/71‑1 (MQ=255) acaGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATCGCGaaa > 1:556490/1‑70 (MQ=255) acaGTACATCATCATCGGCCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATCGCGaaaa < 1:820203/71‑1 (MQ=255) acaGTACATCATCATCGGCCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATCGCGaaaa < 1:2693865/71‑1 (MQ=255) acaGTACATCATCATCGGCCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATCGCGaaaa < 1:4507585/71‑1 (MQ=255) acaGTACATCATCATCGGCCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATCGCGaaaa < 1:2889542/71‑1 (MQ=255) caGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCt < 1:4046899/51‑1 (MQ=255) caGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCt < 1:4743811/51‑1 (MQ=255) catcatcatCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATc > 1:2340329/1‑58 (MQ=255) catcatcatCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATc > 1:2637405/1‑58 (MQ=255) atcatcGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAGCTGATCGCGAAAAAATTCGCgg < 1:2176796/70‑1 (MQ=255) atcatcGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATCGCGAAAAAATTCGCGGt > 1:4044618/1‑71 (MQ=255) tcatcGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATCGCGAAAAAATTcgc > 1:753524/1‑67 (MQ=255) atcGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATCGCGAAAAAATTCGCGGTGCt < 1:5536705/71‑1 (MQ=255) atcGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATCGCGAAAAAATTCGCGGTGCt < 1:5390990/71‑1 (MQ=255) atcGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATCGCGAAAAAATTCGCGGTGCt > 1:3442281/1‑71 (MQ=255) atcGGTCACTCTGAACGTCGTACTTACCACAAAGAATCAGACGAACTGATCGCGAAAAAATTCGCGGTGCt > 1:1384415/1‑71 (MQ=255) | GCATTCACCGGTGAAACCTCTGCTGCTATGCTGAAAGACATCGGCGCACAGTACATCATCATCGGTCACTCTGAACGTCGTACTTACCACAAAGAATCTGACGAACTGATCGCGAAAAAATTCGCGGTGCT > Exported/2320614‑2320744 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |