| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,425,846 | T→A | 8.0% | A82A (GCA→GCT) | rffG ← | dTDP‑glucose 4,6‑dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,425,846 | 0 | T | A | 8.0% | 319.5 / 15.7 | 113 | A82A (GCA→GCT) | rffG | dTDP‑glucose 4,6‑dehydratase |
| Reads supporting (aligned to +/- strand): ref base T (50/54); new base A (3/6); total (53/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
AGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGTTCTGCCCGATCGCAGATAT > Exported/2425777‑2425916 | aGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTg > 1:1189486/1‑71 (MQ=255) tCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGc > 1:2915128/1‑70 (MQ=255) ccGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAg < 1:1554660/71‑1 (MQ=255) gACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGAt > 1:2648124/1‑71 (MQ=255) aCAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGcc < 1:423368/66‑1 (MQ=255) aCAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGAt > 1:4480877/1‑70 (MQ=255) cAATATTGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGc < 1:668410/71‑1 (MQ=255) aaTGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCa > 1:2081447/1‑71 (MQ=255) aaTGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCa > 1:3004546/1‑71 (MQ=255) aaTGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGGCAACATGGCTTTCGGCTGCCAGATGCa < 1:4994585/71‑1 (MQ=255) tGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCAt > 1:1766674/1‑70 (MQ=255) tGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATg < 1:1381664/71‑1 (MQ=255) tGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATg < 1:5304892/71‑1 (MQ=255) tGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATg < 1:1306547/71‑1 (MQ=255) tGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATg < 1:1075914/71‑1 (MQ=255) ttGGTTTCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAGGTGCATCac > 1:4258597/1‑71 (MQ=255) ttGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCa < 1:2187802/61‑1 (MQ=255) ggTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCa > 1:5260986/1‑65 (MQ=255) tttCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAg < 1:846269/71‑1 (MQ=255) tttCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAg < 1:3643897/71‑1 (MQ=255) tCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAgg < 1:4306628/57‑2 (MQ=255) tCAATAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAg > 1:5231748/1‑56 (MQ=255) cAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCt > 1:4457491/1‑71 (MQ=255) aaTAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCt < 1:3665213/70‑1 (MQ=255) aaTAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCt < 1:3736104/70‑1 (MQ=255) aaTAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCt < 1:4878499/70‑1 (MQ=255) aaTAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTg > 1:4198487/1‑71 (MQ=255) aTAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTgg < 1:630480/71‑1 (MQ=255) tAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAggt < 1:4471808/54‑3 (MQ=12) tAAATGCCGCAGGGCCTGTAATTGAACGGTCAACATGGCTTTCAGCAGCCAggt < 1:2181131/54‑3 (MQ=12) aaaCGCTGCCGGGCCGTCAATAGAACGGTAAACATGGCTTTCGGCTGCCa > 1:5329179/1‑50 (MQ=255) aaCGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCt < 1:1390005/70‑1 (MQ=255) aCGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGa < 1:2618622/71‑1 (MQ=255) cGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGAt > 1:2204662/1‑71 (MQ=255) cGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGAt < 1:1752427/71‑1 (MQ=255) tGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCAt < 1:2917800/51‑1 (MQ=255) tGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCAt < 1:2851498/51‑1 (MQ=255) gggCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGt < 1:4754421/71‑1 (MQ=255) ggCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCt < 1:2145930/56‑1 (MQ=255) ggCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTg < 1:12693/71‑1 (MQ=255) ggCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTg < 1:3978996/71‑1 (MQ=255) gCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAg > 1:2883026/1‑68 (MQ=255) gTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTc < 1:1129379/63‑1 (MQ=255) gTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTc < 1:2429509/63‑1 (MQ=255) gTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATa > 1:3336843/1‑71 (MQ=255) tCAATAGTACGGTCAACATGGCTTTCGGCTGCCAGATGCATg < 1:2376346/42‑1 (MQ=255) tCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGt < 1:2928154/65‑1 (MQ=255) tCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGa > 1:1388735/1‑67 (MQ=255) cAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGATACAGt > 1:4380034/1‑48 (MQ=255) cAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGt > 1:1421545/1‑48 (MQ=255) cAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGt > 1:2226470/1‑48 (MQ=255) cAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATAcg > 1:488319/1‑71 (MQ=255) cAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATAcg > 1:2105939/1‑71 (MQ=255) cAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATAcg > 1:4225083/1‑71 (MQ=255) aaTAGTACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGa > 1:2054814/1‑55 (MQ=255) aaTAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGt > 1:2065050/1‑47 (MQ=255) aaTAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATAcgc > 1:597412/1‑71 (MQ=255) aaTAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATAcgc > 1:4631839/1‑71 (MQ=255) aaTAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATAcg < 1:5692899/70‑1 (MQ=255) aaTAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATAcg < 1:1715442/70‑1 (MQ=255) tAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGAcaca < 1:2480473/43‑1 (MQ=255) tAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGaa < 1:4403682/64‑1 (MQ=255) tAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGaa < 1:2139417/64‑1 (MQ=255) tAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGaa < 1:1106705/64‑1 (MQ=255) tAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGt > 1:3381638/1‑71 (MQ=255) tAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGt < 1:2085779/71‑1 (MQ=255) aGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGaa < 1:4624741/63‑1 (MQ=255) gAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGAcaca > 1:2311077/1‑41 (MQ=255) gAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTg > 1:3218616/1‑50 (MQ=255) gAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAAt > 1:4404974/1‑63 (MQ=255) gAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTg < 1:2317884/70‑1 (MQ=255) gAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGc > 1:5694312/1‑71 (MQ=255) cGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAg < 1:5332788/71‑1 (MQ=255) ggTCAACATGGCTTTCAGCAGCCAGGTGCATCACTGCATCCGGCTGATGCTGAGCAAAAATCCGTGCCatt > 1:5561364/1‑69 (MQ=255) gTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGtt < 1:574114/71‑1 (MQ=255) tCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGtt < 1:2930175/70‑1 (MQ=255) tCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGTTc > 1:1733723/1‑71 (MQ=255) aaCATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGaa > 1:1162957/1‑54 (MQ=255) aaCATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGaa > 1:1042855/1‑54 (MQ=255) aCATGGCTTTCGGCTGCCAGATGCATGACACAGTCt < 1:5564246/36‑1 (MQ=255) aCATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGtt < 1:1858464/67‑1 (MQ=255) aTGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTg < 1:2276599/49‑1 (MQ=255) aTGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGTTCt > 1:3564580/1‑67 (MQ=255) aTGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGTTCt > 1:3662243/1‑67 (MQ=255) aTGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGTTCt > 1:4300652/1‑67 (MQ=255) aTGGCTTTCGGCTGCCAGATGCATGACACAGTCTGACTGATGCTCAGTGAATACGCGTGCCAGTTCTGccc > 1:4302114/1‑71 (MQ=255) tGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTc > 1:3330157/1‑44 (MQ=255) tGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGTTCTGCCCg < 1:3470666/71‑1 (MQ=255) ggCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGTTCt < 1:2231048/65‑1 (MQ=255) ggCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGTTCt < 1:367655/65‑1 (MQ=255) ggCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGTTCTGCCCGa > 1:2275775/1‑71 (MQ=255) ggCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGTTCTGCCCGa > 1:1943422/1‑71 (MQ=255) gCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATa > 1:4775192/1‑50 (MQ=255) gCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATa > 1:3423170/1‑50 (MQ=255) cTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGTTCTGCCCGATc > 1:3304099/1‑71 (MQ=255) tttCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGtt < 1:1842939/60‑1 (MQ=255) tttCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGtt < 1:1175585/60‑1 (MQ=255) gCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGtt > 1:4554467/1‑55 (MQ=255) gCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGtt > 1:106692/1‑55 (MQ=255) tGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGTTCTGCCCGATCGCAGatat > 1:3685636/1‑71 (MQ=255) | AGTCCCGACAATGTTGGTTTCAATAAACGCTGCCGGGCCGTCAATAGAACGGTCAACATGGCTTTCGGCTGCCAGATGCATGACACAGTCTGGCTGATGCTCAGTGAATACGCGTGCCAGTTCTGCCCGATCGCAGATAT > Exported/2425777‑2425916 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |