| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,434,323 | T→A | 15.8% | intergenic (+75/‑61) | rhlB → / → gpp | ATP‑dependent RNA helicase/guanosine pentaphosphatase/exopolyphosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,434,323 | 0 | T | A | 15.8% | 209.6 / 29.2 | 82 | intergenic (+75/‑61) | rhlB/gpp | ATP‑dependent RNA helicase/guanosine pentaphosphatase/exopolyphosphatase |
| Reads supporting (aligned to +/- strand): ref base T (38/31); new base A (6/7); total (44/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
ATGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCA > Exported/2434256‑2434393 | aTGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTgtag > 1:2023369/1‑70 (MQ=255) tCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTgtagt < 1:1230803/68‑1 (MQ=255) taTACACAAAATCATTCAAGTTGCATCAAGGCGGCAAGTGAGCGAATCCCGATGAGCTTACTCAGGTAAGt > 1:4293028/2‑71 (MQ=9) aTACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGt > 1:5601956/1‑70 (MQ=255) tACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGa > 1:3953088/1‑71 (MQ=255) tACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGa > 1:195662/1‑71 (MQ=255) tACACAAAATCATTCAAGTTGCATCAAGGCGGCAAGTGAGCGAATCCCGATGAGCTTACTCAGGTAAGTGa < 1:4024673/71‑1 (MQ=9) acAAAATCATTCAAGTTGCATCAAGGCGGCAAGTGAGCGAATCCCGATGAGCTTACTCAGGTAAGTGATTc > 1:2925141/2‑71 (MQ=12) aaaaTCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCa < 1:4193246/70‑1 (MQ=255) aaaaTCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCa > 1:3893752/1‑70 (MQ=255) aaaaTCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAg > 1:5458564/1‑71 (MQ=255) aaaaTCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAg > 1:2517386/1‑71 (MQ=255) aTCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGAtt > 1:4116376/1‑65 (MQ=255) aTCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGt > 1:192398/1‑70 (MQ=255) aTCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGt > 1:5154368/1‑70 (MQ=255) aTTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTg > 1:5533870/1‑59 (MQ=255) aTTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTg > 1:2951237/1‑59 (MQ=255) aTTCAAACTGTATCAAGATGGCAAGAGAATGAATCACGATGAGCTTACTGTAGTAAGTg > 1:3781978/1‑59 (MQ=255) cAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTgtag > 1:5087141/1‑50 (MQ=255) cAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTgtag > 1:1442162/1‑50 (MQ=255) aaCTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTc < 1:5226499/58‑1 (MQ=255) aaCTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTc < 1:2491927/58‑1 (MQ=255) aaCTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTc < 1:4275211/58‑1 (MQ=255) tATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGAtt < 1:55517/52‑1 (MQ=255) tATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAg < 1:3800996/69‑1 (MQ=255) tATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAg < 1:1632874/69‑1 (MQ=255) tATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGc > 1:1956882/1‑70 (MQ=255) tCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCaa > 1:398427/1‑71 (MQ=255) aaGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAaca > 1:2247963/1‑71 (MQ=255) aaGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAac < 1:80674/70‑1 (MQ=255) aGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAaca > 1:4882327/1‑70 (MQ=255) gATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGaa > 1:3420785/1‑58 (MQ=255) tGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAAcaca > 1:4886431/1‑69 (MQ=255) cggCAAGTGAGCGAATCCCGATGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCaga < 1:3762691/70‑1 (MQ=2) gCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAgaga < 1:769099/71‑1 (MQ=255) agagAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAg < 1:5298159/71‑1 (MQ=255) agagAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAg < 1:4304180/71‑1 (MQ=255) agagAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAg < 1:2893665/71‑1 (MQ=255) gagaATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCaa > 1:279177/1‑59 (MQ=255) gaatgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGcc > 1:4161099/1‑55 (MQ=255) aatgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATg < 1:2961155/49‑1 (MQ=255) aatgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATg < 1:4740567/49‑1 (MQ=255) aatgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGAtt > 1:4042398/1‑70 (MQ=255) aatgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTg < 1:5089868/71‑1 (MQ=255) atgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAg < 1:1837188/66‑1 (MQ=255) atgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTg < 1:1370517/70‑1 (MQ=255) tgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGaa > 1:1316831/1‑45 (MQ=255) tgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGcc > 1:3701032/1‑52 (MQ=255) tgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGcc > 1:4582472/1‑52 (MQ=255) tgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGcc > 1:724454/1‑52 (MQ=255) tgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTg < 1:955518/69‑1 (MQ=255) gaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAacac < 1:4405530/56‑1 (MQ=255) aatCCCGATGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAg > 1:2506493/1‑70 (MQ=9) aatCCCGATGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAg > 1:1530604/1‑70 (MQ=9) atCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACag > 1:3718940/1‑56 (MQ=255) atCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACag > 1:672959/1‑56 (MQ=255) atCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACag > 1:5572557/1‑56 (MQ=255) cccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGa > 1:1279910/1‑69 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATg < 1:3958831/70‑1 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATg < 1:121317/70‑1 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATg > 1:594413/1‑70 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATg > 1:3322455/1‑70 (MQ=255) ccGATGAGCTTACTGTAGTAAGTGATTCAGGGGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATg < 1:2059265/70‑1 (MQ=255) ccGATGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATg > 1:3565310/1‑70 (MQ=9) ccGATGAGCTCACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATg < 1:4671174/70‑1 (MQ=255) cGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGaa < 1:1773347/71‑1 (MQ=255) aTGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGaa > 1:5325034/1‑69 (MQ=255) aTGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATg < 1:1367101/67‑1 (MQ=9) aTGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATg < 1:128231/67‑1 (MQ=9) aTGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATg < 1:1081083/67‑1 (MQ=9) tGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag < 1:3058619/71‑1 (MQ=255) tGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag < 1:564370/71‑1 (MQ=255) tGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag < 1:5655139/71‑1 (MQ=255) gAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGCGACAGATTGAAGGATGAagag > 1:2168033/1‑70 (MQ=255) gAGCTTACTCAGGTAAGTGATTCGTGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATGAagtg > 1:3677193/1‑68 (MQ=255) aGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTa > 1:3899367/1‑71 (MQ=255) aGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATGAAgtgt < 1:2636176/70‑1 (MQ=9) cTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACaga < 1:3382326/46‑1 (MQ=255) cTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACaga < 1:567898/46‑1 (MQ=255) ttACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACaga < 1:1212478/45‑1 (MQ=255) ttACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACag < 1:5697365/44‑1 (MQ=255) tACTGTAGTAAGTGATTCAGGTGAAAGAATGAAGCCaa > 1:2602198/1‑38 (MQ=255) aCTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCa < 1:4020884/36‑1 (MQ=255) aCTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAgaga > 1:3698710/1‑45 (MQ=255) aCTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAgaga > 1:4518654/1‑45 (MQ=255) aCTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTa > 1:5067942/1‑66 (MQ=255) gtagtaAGTGATTCACGTGAAAGAATGCAGCCAACACag > 1:3747104/1‑39 (MQ=255) tagtaAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCa > 1:2404347/1‑71 (MQ=255) | ATGTCATACTCAAAATCATTCAAACTGTATCAAGATGGCAAGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCA > Exported/2434256‑2434393 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |