Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,434,365 A→G 13.6% intergenic (+117/‑19) rhlB → / → gpp ATP‑dependent RNA helicase/guanosine pentaphosphatase/exopolyphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,434,3650AG13.6% 172.7 / 15.2 66intergenic (+117/‑19)rhlB/gppATP‑dependent RNA helicase/guanosine pentaphosphatase/exopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base A (34/23);  new base G (4/5);  total (38/28)
Fisher's exact test for biased strand distribution p-value = 4.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

AGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGCAGCCATTGATCTCGGTTCGAATAG  >  Exported/2434296‑2434433
                                                                     |                                                                    
agagAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAg                                                                     <  1:4304180/71‑1 (MQ=255)
agagAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAg                                                                     <  1:5298159/71‑1 (MQ=255)
agagAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAg                                                                     <  1:2893665/71‑1 (MQ=255)
    aatgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGAtt                                                                  >  1:4042398/1‑70 (MQ=255)
    aatgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTg                                                                 <  1:5089868/71‑1 (MQ=255)
     atgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAg                                                                     <  1:1837188/66‑1 (MQ=255)
     atgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTg                                                                 <  1:1370517/70‑1 (MQ=255)
      tgaatCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTg                                                                 <  1:955518/69‑1 (MQ=255)
        aatCCCGATGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAg                                                              >  1:2506493/1‑70 (MQ=9)
        aatCCCGATGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAg                                                              >  1:1530604/1‑70 (MQ=9)
           cccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGa                                                            >  1:1279910/1‑69 (MQ=255)
            ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATg                                                          >  1:594413/1‑70 (MQ=255)
            ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATg                                                          <  1:3958831/70‑1 (MQ=255)
            ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATg                                                          >  1:3322455/1‑70 (MQ=255)
            ccGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATg                                                          <  1:121317/70‑1 (MQ=255)
            ccGATGAGCTTACTGTAGTAAGTGATTCAGGGGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATg                                                          <  1:2059265/70‑1 (MQ=255)
            ccGATGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATg                                                          >  1:3565310/1‑70 (MQ=9)
            ccGATGAGCTCACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATg                                                          <  1:4671174/70‑1 (MQ=255)
             cGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGaa                                                        <  1:1773347/71‑1 (MQ=255)
               aTGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGaa                                                        >  1:5325034/1‑69 (MQ=255)
               aTGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATg                                                          <  1:1367101/67‑1 (MQ=9)
               aTGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATg                                                          <  1:128231/67‑1 (MQ=9)
               aTGAGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATg                                                          <  1:1081083/67‑1 (MQ=9)
                tGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag                                                     <  1:3058619/71‑1 (MQ=255)
                tGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag                                                     <  1:564370/71‑1 (MQ=255)
                tGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAagag                                                     <  1:5655139/71‑1 (MQ=255)
                 gAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGCGACAGATTGAAGGATGAagag                                                     >  1:2168033/1‑70 (MQ=255)
                 gAGCTTACTCAGGTAAGTGATTCGTGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATGAagtg                                                     >  1:3677193/1‑68 (MQ=255)
                  aGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTa                                                   >  1:3899367/1‑71 (MQ=255)
                  aGCTTACTCAGGTAAGTGATTCGGGGGAGCGAACGCAGCCAAGGCAGAGGCGGCTTGAAGGATGAAgtgt                                                    <  1:2636176/70‑1 (MQ=9)
                       aCTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTa                                                   >  1:5067942/1‑66 (MQ=255)
                           tagtaAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCa                                          >  1:2404347/1‑71 (MQ=255)
                                aGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGa                                                            >  1:1790166/1‑48 (MQ=255)
                                  tGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCtcgtc                                   >  1:2891341/1‑71 (MQ=255)
                                  tGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCtcgtc                                   >  1:2311966/1‑71 (MQ=255)
                                  tGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCtcgtc                                   >  1:1550582/1‑71 (MQ=255)
                                  tGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATCAAGAGTATGGGTTCCACAtcgtc                                   >  1:5458551/1‑71 (MQ=255)
                                   gATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCtcgtcg                                  >  1:4667734/1‑71 (MQ=255)
                                     ttCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGaa                                                        <  1:3243562/47‑1 (MQ=255)
                                     ttCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGaa                                                        <  1:1675654/47‑1 (MQ=255)
                                     ttCAGGGGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGaa                                                        <  1:3009514/47‑1 (MQ=255)
                                       cAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTg                               <  1:5350950/70‑1 (MQ=255)
                                        aGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGt                              <  1:2491598/70‑1 (MQ=255)
                                           tGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGaa                                                        >  1:3101460/1‑41 (MQ=255)
                                           tGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGc                          <  1:3294857/71‑1 (MQ=255)
                                            gAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATggg                                               >  1:3942289/1‑49 (MQ=255)
                                            gAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATggg                                               >  1:1985509/1‑49 (MQ=255)
                                             aaaGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATggg                                               >  1:962305/1‑48 (MQ=255)
                                             aaaGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATggg                                               >  1:1439269/1‑48 (MQ=255)
                                             aaaGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATggg                                               >  1:922984/1‑48 (MQ=255)
                                             aaaGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATggg                                               >  1:5610772/1‑48 (MQ=255)
                                             aaaGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATggg                                               >  1:4383222/1‑48 (MQ=255)
                                             aaaGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATgg                                                >  1:4693506/1‑47 (MQ=255)
                                             aaaGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATgg                                                >  1:3802152/1‑47 (MQ=255)
                                                gAATGCAGCCAACACAGAGAGAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGCAGCCa                     >  1:4839376/1‑71 (MQ=255)
                                                gAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGCAGCCa                     >  1:1944370/1‑71 (MQ=255)
                                                gAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGCAGCCa                     >  1:1017520/1‑71 (MQ=255)
                                                 aaTGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCt                                       >  1:2115110/1‑52 (MQ=255)
                                                 aaTGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCt                                       >  1:2340350/1‑52 (MQ=255)
                                                 aaTGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCt                                       >  1:2739377/1‑52 (MQ=255)
                                                   tGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGCAGCCATTg                  <  1:2741606/71‑1 (MQ=255)
                                                    gCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGCAGCCATTg                  <  1:4640623/70‑1 (MQ=255)
                                                                gagaCAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCt                                >  1:4109164/1‑44 (MQ=255)
                                                                gagaCAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGCAGCCATTGATCTCGGTTCGaa     <  1:2745846/71‑1 (MQ=255)
                                                                gagaCAGATTGAAGGATGAAGAGTATGGGTTCCAACTCGTCGc                                 >  1:2279848/1‑43 (MQ=255)
                                                                   aCAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGCAGCCATTGATCTCGGTTCGAATAg  >  1:1756418/1‑71 (MQ=255)
                                                                     |                                                                    
AGAGAATGAATCCCGATGAGCTTACTGTAGTAAGTGATTCAGGTGAAAGAATGCAGCCAACACAGAGACAGATTGAAGGATGAAGAGTATGGGTTCCACCTCGTCGCTGTATGCAGCCATTGATCTCGGTTCGAATAG  >  Exported/2434296‑2434433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: