BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,562,530	T→C	8.8%	K516R (AAA→AGA) ‡	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,562,530	0	T	C	8.8%	343.7 / 17.3	124	K516R (AAA→AGA) ‡	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base T (54/59); new base C (4/7); total (58/66)
Fisher's exact test for biased strand distribution p-value = 5.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 132 total aligned reads displayed.

GGTGATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGC  >  Exported/2562460‑2562599

ggtgATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCtt                                                                       >  1:4011431/1‑71 (MQ=255)

 gtgATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCttt                                                                      >  1:1093471/1‑71 (MQ=255)

 gtgATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCttt                                                                      >  1:2627907/1‑71 (MQ=255)

  tgATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTg                                                                     <  1:4195580/71‑1 (MQ=255)

    aTCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGcc                                                                   >  1:5326431/1‑71 (MQ=255)

       aCGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCccac                                                                <  1:5276933/71‑1 (MQ=255)

        acgccACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCccac                                                                <  1:4110805/67‑1 (MQ=255)

             aCTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGcc                                                          >  1:721085/1‑71 (MQ=255)

              cTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGc                                                           <  1:3262212/69‑1 (MQ=255)

               tataCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTa                                                        >  1:2252248/1‑71 (MQ=255)

                ataCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCccacca                                                              >  1:2364439/1‑64 (MQ=255)

                 taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAcc                                                      >  1:1960819/1‑71 (MQ=255)

                 taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAc                                                       >  1:3234453/1‑70 (MQ=255)

                 taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAc                                                       >  1:3215675/1‑70 (MQ=255)

                   cAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccg                                                     <  1:4714091/70‑1 (MQ=255)

                   cAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccg                                                     <  1:624641/70‑1 (MQ=255)

                    aGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgcc                                                   <  1:2362189/71‑1 (MQ=255)

                     ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                  <  1:2667496/71‑1 (MQ=255)

                     ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                  <  1:4217252/71‑1 (MQ=255)

                     ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgcc                                                   <  1:556458/70‑1 (MQ=255)

                       tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                <  1:756139/71‑1 (MQ=255)

                       tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                >  1:4525704/1‑71 (MQ=255)

                       tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                >  1:1357120/1‑71 (MQ=255)

                       tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                <  1:398186/71‑1 (MQ=255)

                        gAAGATCAGGCTGTGTTTGACATTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                >  1:4322132/1‑70 (MQ=255)

                         aaGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGc                                                                    <  1:4586898/49‑1 (MQ=255)

                           gATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                             <  1:1825952/70‑1 (MQ=255)

                            aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                             <  1:280348/69‑1 (MQ=255)

                            aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                           <  1:1229224/71‑1 (MQ=255)

                            aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                           <  1:1243591/71‑1 (MQ=255)

                             tCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCccacca                                                              >  1:5674764/1‑51 (MQ=255)

                             tCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCccacca                                                              >  1:2037440/1‑51 (MQ=255)

                             tCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCccacca                                                              >  1:5355154/1‑51 (MQ=255)

                             tCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                           >  1:4296573/1‑70 (MQ=255)

                              cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGc                                                                    >  1:1502105/1‑44 (MQ=255)

                              cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGc                                                                    >  1:2708075/1‑44 (MQ=255)

                              cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGc                                                                    >  1:1588066/1‑44 (MQ=255)

                              cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                         >  1:772871/1‑71 (MQ=255)

                              cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                         >  1:3764316/1‑71 (MQ=255)

                                 gCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatag                                      <  1:4509397/71‑1 (MQ=255)

                                  cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                     <  1:4318108/71‑1 (MQ=255)

                                  cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                     <  1:1613557/71‑1 (MQ=255)

                                  cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                     <  1:3044874/71‑1 (MQ=255)

                                  cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                     <  1:3656059/71‑1 (MQ=255)

                                     tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgat                                  <  1:1353602/71‑1 (MQ=255)

                                     tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                   <  1:5121931/70‑1 (MQ=255)

                                     tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                        <  1:3451277/65‑1 (MQ=255)

                                      gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                   >  1:1374445/1‑69 (MQ=255)

                                      gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                   >  1:169429/1‑69 (MQ=255)

                                      gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                          <  1:1320160/62‑1 (MQ=255)

                                      gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                          <  1:2046781/62‑1 (MQ=255)

                                       tttGACAGTAAAGCTGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                 <  1:2911125/70‑1 (MQ=255)

                                       tttGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                   >  1:3311967/1‑68 (MQ=255)

                                       tttGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGcgatagcgatag                                <  1:4068233/71‑1 (MQ=255)

                                         tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                >  1:606529/1‑53 (MQ=255)

                                         tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                >  1:1462011/1‑53 (MQ=255)

                                          gACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGcaca                                              >  1:5479547/1‑54 (MQ=255)

                                          gACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                              >  1:630133/1‑70 (MQ=255)

                                          caCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                              >  1:4225911/2‑70 (MQ=255)

                                             aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                           >  1:1313452/1‑54 (MQ=255)

                                             aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                          <  1:4966100/71‑1 (MQ=255)

                                             aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                          <  1:1487587/71‑1 (MQ=255)

                                              gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                         <  1:93396/71‑1 (MQ=255)

                                               tAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                 <  1:4692752/62‑1 (MQ=255)

                                                 aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgggg                       <  1:1311113/70‑1 (MQ=255)

                                                  aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgata                                       <  1:980012/53‑1 (MQ=255)

                                                  aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                        >  1:5267372/1‑68 (MQ=255)

                                                   gCGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCggg                        >  1:296299/1‑67 (MQ=255)

                                                   gCGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGgag                     <  1:3349636/70‑1 (MQ=255)

                                                     ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                   >  1:702624/1‑70 (MQ=255)

                                                     ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                   >  1:2565244/1‑70 (MQ=255)

                                                     ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                   >  1:5483102/1‑70 (MQ=255)

                                                      gAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                 >  1:856635/1‑71 (MQ=255)

                                                       aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGcac                                               <  1:4887408/40‑1 (MQ=255)

                                                       aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                         <  1:1567484/46‑1 (MQ=255)

                                                       aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                 <  1:518136/70‑1 (MQ=255)

                                                        aCAAATCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                 <  1:3619352/37‑1 (MQ=255)

                                                        aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                         >  1:4716623/1‑45 (MQ=255)

                                                        aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGgag                     >  1:2561236/1‑65 (MQ=255)

                                                        aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGAGATAGATTGCGGGgag                     >  1:740332/1‑65 (MQ=255)

                                                         cAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                             >  1:2525723/1‑40 (MQ=255)

                                                         cAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                        <  1:3740631/61‑1 (MQ=255)

                                                          agttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCtt             >  1:267579/4‑71 (MQ=255)

                                                          aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                 <  1:5156539/35‑1 (MQ=255)

                                                          aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                 <  1:1256531/35‑1 (MQ=255)

                                                          aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                 <  1:2488766/35‑1 (MQ=255)

                                                           aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa            >  1:3274186/1‑71 (MQ=255)

                                                            ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCg                          <  1:1172219/55‑1 (MQ=255)

                                                            ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttgc           <  1:3223874/70‑3 (MQ=255)

                                                            aTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCCATAGATTGCGGGGAGATCATCTTAc           >  1:5497410/1‑71 (MQ=255)

                                                             tCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCg                          >  1:3907274/1‑55 (MQ=255)

                                                             tCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttg            <  1:5010779/69‑2 (MQ=255)

                                                             tCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAcc          <  1:1158933/71‑1 (MQ=255)

                                                              cAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGcc          <  1:941088/70‑1 (MQ=255)

                                                                 aCTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGt       <  1:3799749/70‑1 (MQ=255)

                                                                   tctTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                 <  1:2796059/42‑1 (MQ=255)

                                                                   gctTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGgag                     >  1:1170062/2‑54 (MQ=255)

                                                                     tttGCCCACCAGGCCTACCGCCGCACAGGcgatagcgatag                                <  1:1825887/41‑1 (MQ=255)

                                                                     tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcatc               >  1:4028601/1‑58 (MQ=255)

                                                                     tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc  >  1:5659472/1‑71 (MQ=255)

GGTGATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGC  >  Exported/2562460‑2562599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`