BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,562,531	T→G	8.8%	K516Q (AAA→CAA) ‡	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,562,531	0	T	G	8.8%	308.4 / 21.3	124	K516Q (AAA→CAA) ‡	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base T (54/59); new base G (4/7); total (58/66)
Fisher's exact test for biased strand distribution p-value = 5.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

GTGATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGC  >  Exported/2562461‑2562599

gtgATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCttt                                                                      >  1:2627907/1‑71 (MQ=255)

gtgATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCttt                                                                      >  1:1093471/1‑71 (MQ=255)

 tgATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTg                                                                     <  1:4280584/71‑1 (MQ=255)

 tgATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTg                                                                     <  1:4195580/71‑1 (MQ=255)

  gATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGc                                                                    >  1:3097850/1‑71 (MQ=255)

   aTCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGcc                                                                   >  1:5326431/1‑71 (MQ=255)

    tCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGccc                                                                  <  1:2682002/71‑1 (MQ=255)

      aCGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCccac                                                                <  1:5276933/71‑1 (MQ=255)

       acgccACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCccac                                                                <  1:4110805/67‑1 (MQ=255)

            aCTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGcc                                                          <  1:250831/71‑1 (MQ=255)

            aCTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGcc                                                          >  1:721085/1‑71 (MQ=255)

             cTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAg                                                             <  1:3891471/67‑1 (MQ=255)

             cTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGc                                                           <  1:3262212/69‑1 (MQ=255)

              tataCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTa                                                        >  1:2252248/1‑71 (MQ=255)

               ataCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCccacca                                                              >  1:2364439/1‑64 (MQ=255)

                taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAcc                                                      >  1:286219/1‑71 (MQ=255)

                taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAcc                                                      >  1:1960819/1‑71 (MQ=255)

                taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAc                                                       >  1:3234453/1‑70 (MQ=255)

                taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAc                                                       >  1:3215675/1‑70 (MQ=255)

                  cAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccg                                                     <  1:4714091/70‑1 (MQ=255)

                  cAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccg                                                     <  1:624641/70‑1 (MQ=255)

                   aGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgcc                                                   <  1:2362189/71‑1 (MQ=255)

                    ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgcc                                                   <  1:556458/70‑1 (MQ=255)

                      tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                >  1:1357120/1‑71 (MQ=255)

                      tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                <  1:398186/71‑1 (MQ=255)

                      tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                <  1:756139/71‑1 (MQ=255)

                      tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                >  1:4525704/1‑71 (MQ=255)

                       gAAGATCAGGCTGTGTTTGACATTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                >  1:4322132/1‑70 (MQ=255)

                        aaGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGc                                                                    <  1:4586898/49‑1 (MQ=255)

                          gATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                             <  1:1825952/70‑1 (MQ=255)

                           aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                             <  1:280348/69‑1 (MQ=255)

                           aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                           <  1:1243591/71‑1 (MQ=255)

                           aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                           <  1:1229224/71‑1 (MQ=255)

                            tCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCccacca                                                              >  1:5674764/1‑51 (MQ=255)

                            tCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCccacca                                                              >  1:5355154/1‑51 (MQ=255)

                            tCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCccacca                                                              >  1:2037440/1‑51 (MQ=255)

                            tCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                           >  1:4296573/1‑70 (MQ=255)

                             cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGc                                                                    >  1:2708075/1‑44 (MQ=255)

                             cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGc                                                                    >  1:1502105/1‑44 (MQ=255)

                             cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGc                                                                    >  1:1588066/1‑44 (MQ=255)

                             cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                         >  1:3764316/1‑71 (MQ=255)

                             cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                         >  1:772871/1‑71 (MQ=255)

                             cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                         >  1:4599883/1‑71 (MQ=255)

                                 cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                     <  1:1613557/71‑1 (MQ=255)

                                 cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                     <  1:3044874/71‑1 (MQ=255)

                                 cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                     <  1:3656059/71‑1 (MQ=255)

                                    tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgat                                  <  1:1353602/71‑1 (MQ=255)

                                     gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                   >  1:1374445/1‑69 (MQ=255)

                                     gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                   >  1:169429/1‑69 (MQ=255)

                                     gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                          <  1:1320160/62‑1 (MQ=255)

                                     gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                          <  1:2046781/62‑1 (MQ=255)

                                      tttGACAGTAAAGCTGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                 <  1:2911125/70‑1 (MQ=255)

                                      tttGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                   >  1:3311967/1‑68 (MQ=255)

                                        tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                >  1:606529/1‑53 (MQ=255)

                                        tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                >  1:1462011/1‑53 (MQ=255)

                                         gACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGcaca                                              >  1:5479547/1‑54 (MQ=255)

                                         gACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                              >  1:630133/1‑70 (MQ=255)

                                         caCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                              >  1:4225911/2‑70 (MQ=255)

                                          aCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAtt                             <  1:2627415/70‑1 (MQ=255)

                                           cAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGc                           <  1:3321416/71‑1 (MQ=255)

                                            aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                           >  1:1313452/1‑54 (MQ=255)

                                            aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                          <  1:4966100/71‑1 (MQ=255)

                                            aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                          <  1:1487587/71‑1 (MQ=255)

                                             gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                         <  1:93396/71‑1 (MQ=255)

                                                aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgggg                       <  1:1311113/70‑1 (MQ=255)

                                                 aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgata                                       <  1:980012/53‑1 (MQ=255)

                                                 aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                        >  1:5267372/1‑68 (MQ=255)

                                                  gCGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGgag                     <  1:3349636/70‑1 (MQ=255)

                                                   cGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                   <  1:3629734/71‑1 (MQ=255)

                                                    ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                   >  1:4808613/1‑70 (MQ=255)

                                                    ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                   >  1:702624/1‑70 (MQ=255)

                                                    ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                   >  1:2565244/1‑70 (MQ=255)

                                                    ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                   >  1:5483102/1‑70 (MQ=255)

                                                     gAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                 >  1:856635/1‑71 (MQ=255)

                                                      aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                         <  1:1567484/46‑1 (MQ=255)

                                                      aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                 <  1:518136/70‑1 (MQ=255)

                                                       aCAAATCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                 <  1:3619352/37‑1 (MQ=255)

                                                       aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                         >  1:4716623/1‑45 (MQ=255)

                                                       aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcatc               >  1:4026579/1‑71 (MQ=255)

                                                       aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGAGATAGATTGCGGGgag                     >  1:740332/1‑65 (MQ=255)

                                                        cAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                             >  1:2525723/1‑40 (MQ=255)

                                                         agttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCtt             >  1:267579/4‑71 (MQ=255)

                                                         aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                 <  1:1256531/35‑1 (MQ=255)

                                                         aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                 <  1:5156539/35‑1 (MQ=255)

                                                           ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCg                          <  1:1172219/55‑1 (MQ=255)

                                                           ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttg            <  1:3259735/69‑2 (MQ=255)

                                                           ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttg            <  1:4195173/69‑2 (MQ=255)

                                                           aTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCCATAGATTGCGGGGAGATCATCTTAc           >  1:5497410/1‑71 (MQ=255)

                                                            tCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttg            <  1:5010779/69‑2 (MQ=255)

                                                            tCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAcc          <  1:1158933/71‑1 (MQ=255)

                                                             cAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttgc           >  1:5118931/1‑67 (MQ=255)

                                                             cAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGcc          <  1:941088/70‑1 (MQ=255)

                                                                aCTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGt       <  1:3799749/70‑1 (MQ=255)

                                                                  gctTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGgag                     >  1:1170062/2‑54 (MQ=255)

                                                                    tttGCCCACCAGGCCTACCGCCGCACAGGcgatagcgatag                                <  1:1825887/41‑1 (MQ=255)

                                                                    tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcatc               >  1:4028601/1‑58 (MQ=255)

                                                                    tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc  >  1:5659472/1‑71 (MQ=255)

                                                                    tttGCCCACCAGGACTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc  >  1:4463872/1‑71 (MQ=255)

                                                                      tGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                   >  1:4490880/1‑36 (MQ=255)

                                                                      tGCCCACCAGCCCTACCGCCGCACAGgcgatagcga                                   >  1:2539022/1‑36 (MQ=255)

GTGATCACGCCCACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGC  >  Exported/2562461‑2562599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`