BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,562,540	G→T	9.7%	L513M (CTG→ATG)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,562,540	0	G	T	9.7%	387.4 / 15.7	123	L513M (CTG→ATG)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base G (54/56); new base T (4/8); total (58/65)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01

Only 100 of 128 total aligned reads displayed.

ACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTA  >  Exported/2562473‑2562610

aCTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGcc                                                                     <  1:250831/71‑1 (MQ=255)

aCTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGcc                                                                     >  1:721085/1‑71 (MQ=255)

 cTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGc                                                                      <  1:3262212/69‑1 (MQ=255)

    taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTa                                                                   <  1:527234/69‑1 (MQ=255)

    taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAcc                                                                 >  1:1960819/1‑71 (MQ=255)

    taCAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAc                                                                  >  1:3215675/1‑70 (MQ=255)

      cAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccg                                                                <  1:624641/70‑1 (MQ=255)

      cAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccg                                                                <  1:4714091/70‑1 (MQ=255)

       aGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgcc                                                              <  1:2362189/71‑1 (MQ=255)

        ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                             <  1:4217252/71‑1 (MQ=255)

        ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                             <  1:2667496/71‑1 (MQ=255)

        ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgcc                                                              <  1:556458/70‑1 (MQ=255)

          tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                           <  1:756139/71‑1 (MQ=255)

          tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                           <  1:398186/71‑1 (MQ=255)

          tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                           >  1:4525704/1‑71 (MQ=255)

           gAAGATCAGGCTGTGTTTGACATTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                           >  1:4322132/1‑70 (MQ=255)

              gATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                                        <  1:1825952/70‑1 (MQ=255)

               aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                             <  1:1260010/64‑1 (MQ=255)

               aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                                        <  1:280348/69‑1 (MQ=255)

               aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                      <  1:1243591/71‑1 (MQ=255)

                tCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                      >  1:4296573/1‑70 (MQ=255)

                 cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                    >  1:3764316/1‑71 (MQ=255)

                 cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                    >  1:5518617/1‑71 (MQ=255)

                 cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                    >  1:772871/1‑71 (MQ=255)

                    gCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatag                                                 <  1:4509397/71‑1 (MQ=255)

                     cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                <  1:3044874/71‑1 (MQ=255)

                     cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                <  1:4318108/71‑1 (MQ=255)

                     cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                <  1:3656059/71‑1 (MQ=255)

                        tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgat                                             <  1:1353602/71‑1 (MQ=255)

                        tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                              <  1:5121931/70‑1 (MQ=255)

                        tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                   <  1:3451277/65‑1 (MQ=255)

                         gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                              >  1:169429/1‑69 (MQ=255)

                         gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                              >  1:1374445/1‑69 (MQ=255)

                         gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                                     <  1:1202237/62‑1 (MQ=255)

                         gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                                     <  1:2046781/62‑1 (MQ=255)

                         gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                                     <  1:1320160/62‑1 (MQ=255)

                          tttGACAGTAAAGCTGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                            <  1:2911125/70‑1 (MQ=255)

                          tttGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                              >  1:3311967/1‑68 (MQ=255)

                            tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                           >  1:1462011/1‑53 (MQ=255)

                            tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                           >  1:606529/1‑53 (MQ=255)

                            tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                                         >  1:1335316/1‑71 (MQ=255)

                             gACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGcaca                                                         >  1:5479547/1‑54 (MQ=255)

                              aCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAtt                                        <  1:581073/70‑1 (MQ=255)

                              aCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAtt                                        <  1:2627415/70‑1 (MQ=255)

                                aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                      >  1:5369751/1‑54 (MQ=255)

                                aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                                     <  1:4966100/71‑1 (MQ=255)

                                 gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                            <  1:678832/47‑1 (MQ=255)

                                 gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                                    <  1:93396/71‑1 (MQ=255)

                                  tAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                            <  1:4692752/62‑1 (MQ=255)

                                    aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgggg                                  <  1:1311113/70‑1 (MQ=255)

                                     aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgata                                                  <  1:980012/53‑1 (MQ=255)

                                     aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                   >  1:5267372/1‑68 (MQ=255)

                                      gCGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCggg                                   >  1:296299/1‑67 (MQ=255)

                                      gCGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGgag                                <  1:3349636/70‑1 (MQ=255)

                                      gCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                   >  1:156403/1‑67 (MQ=255)

                                        ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                              >  1:2565244/1‑70 (MQ=255)

                                        ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                              >  1:4808613/1‑70 (MQ=255)

                                        ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                              >  1:702624/1‑70 (MQ=255)

                                         gAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                            >  1:856635/1‑71 (MQ=255)

                                          aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGcac                                                          <  1:4887408/40‑1 (MQ=255)

                                          aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                    <  1:1567484/46‑1 (MQ=255)

                                           aCAAATCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                            <  1:3619352/37‑1 (MQ=255)

                                           aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                    >  1:4716623/1‑45 (MQ=255)

                                           aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGgag                                >  1:2561236/1‑65 (MQ=255)

                                           aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcatc                          >  1:4026579/1‑71 (MQ=255)

                                            cAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                                        >  1:2525723/1‑40 (MQ=255)

                                            cAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                   <  1:3740631/61‑1 (MQ=255)

                                             aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                            <  1:5156539/35‑1 (MQ=255)

                                             aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                            <  1:2488766/35‑1 (MQ=255)

                                             aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                            <  1:1256531/35‑1 (MQ=255)

                                              aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                       >  1:3274186/1‑71 (MQ=255)

                                              aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                       >  1:753411/1‑71 (MQ=255)

                                               ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCg                                     <  1:1172219/55‑1 (MQ=255)

                                               ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttgc                      <  1:3223874/70‑3 (MQ=255)

                                               ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttg                       <  1:4195173/69‑2 (MQ=255)

                                               ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttg                       <  1:3259735/69‑2 (MQ=255)

                                               aTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCCATAGATTGCGGGGAGATCATCTTAc                      >  1:5497410/1‑71 (MQ=255)

                                                tCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCg                                     >  1:3907274/1‑55 (MQ=255)

                                                tCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAcc                     <  1:1158933/71‑1 (MQ=255)

                                                 cAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttgc                      >  1:5118931/1‑67 (MQ=255)

                                                 cAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGcc                     <  1:941088/70‑1 (MQ=255)

                                                  aGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCgg                   >  1:1643765/1‑71 (MQ=255)

                                                    aCTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGt                  <  1:3799749/70‑1 (MQ=255)

                                                      tctTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                            <  1:2796059/42‑1 (MQ=255)

                                                      gctTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGgag                                >  1:1170062/2‑54 (MQ=255)

                                                        tttGCCCACCAGGCCTACCGCCGCACAGGcgatagcgatag                                           <  1:1825887/41‑1 (MQ=255)

                                                        tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcatc                          >  1:4028601/1‑58 (MQ=255)

                                                        tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc             >  1:5659472/1‑71 (MQ=255)

                                                        tttGCCCACCAGGACTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc             >  1:4463872/1‑71 (MQ=255)

                                                          tGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                              >  1:4490880/1‑36 (MQ=255)

                                                           gCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGccgc          <  1:4157060/71‑3 (MQ=255)

                                                           gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGa                >  1:3194293/1‑65 (MQ=255)

                                                           gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc          >  1:5111582/1‑71 (MQ=255)

                                                           gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc          >  1:1941283/1‑71 (MQ=255)

                                                            cccACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc          <  1:2776999/70‑1 (MQ=255)

                                                             ccaccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCg        >  1:3093636/1‑71 (MQ=255)

                                                              caccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcat                           <  1:1412731/51‑1 (MQ=255)

                                                                ccaCGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                        <  1:4749875/52‑1 (MQ=255)

                                                                 caGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg    <  1:1457428/71‑1 (MQ=255)

                                                                   ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                              >  1:15323/1‑43 (MQ=255)

ACTATACAGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTA  >  Exported/2562473‑2562610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`