BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,562,550	C→G	10.7%	A509A (GCG→GCC)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,562,550	0	C	G	10.7%	303.9 / 20.5	112	A509A (GCG→GCC)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base C (52/47); new base G (4/8); total (57/55)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

AGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGG  >  Exported/2562480‑2562617

aGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgcc                                                                     <  1:2362189/71‑1 (MQ=255)

 ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                                    <  1:4217252/71‑1 (MQ=255)

 ggTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgcc                                                                     <  1:556458/70‑1 (MQ=255)

   tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                  <  1:398186/71‑1 (MQ=255)

   tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                  >  1:4525704/1‑71 (MQ=255)

   tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                  >  1:1357120/1‑71 (MQ=255)

   tGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                  <  1:756139/71‑1 (MQ=255)

    gAAGATCAGGCTGTGTTTGACATTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                  >  1:4322132/1‑70 (MQ=255)

       gATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                                               <  1:1825952/70‑1 (MQ=255)

        aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTAccgccg                                                                    <  1:1260010/64‑1 (MQ=255)

        aTCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                             <  1:1243591/71‑1 (MQ=255)

         tCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                             >  1:4296573/1‑70 (MQ=255)

          cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                           >  1:4599883/1‑71 (MQ=255)

          cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                           >  1:772871/1‑71 (MQ=255)

          cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                           >  1:5518617/1‑71 (MQ=255)

          cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                           >  1:3764316/1‑71 (MQ=255)

              cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                       <  1:3656059/71‑1 (MQ=255)

              cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                       <  1:4318108/71‑1 (MQ=255)

              cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                       <  1:1613557/71‑1 (MQ=255)

              cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                       <  1:3044874/71‑1 (MQ=255)

                 tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                          <  1:3451277/65‑1 (MQ=255)

                  gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                     >  1:169429/1‑69 (MQ=255)

                  gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                     >  1:1374445/1‑69 (MQ=255)

                  gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                                            <  1:2046781/62‑1 (MQ=255)

                  gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                                            <  1:1320160/62‑1 (MQ=255)

                  gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                                            <  1:1202237/62‑1 (MQ=255)

                   tttGACAGTAAAGCTGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                                   <  1:2911125/70‑1 (MQ=255)

                   tttGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                     >  1:3311967/1‑68 (MQ=255)

                   tttGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGcgatagcgatag                                                  <  1:4068233/71‑1 (MQ=255)

                     tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                  >  1:606529/1‑53 (MQ=255)

                     tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGca                                                                  >  1:1462011/1‑53 (MQ=255)

                     tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                                                >  1:1335316/1‑71 (MQ=255)

                      gACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGcaca                                                                >  1:5479547/1‑54 (MQ=255)

                      caCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                                                >  1:4225911/2‑70 (MQ=255)

                       aCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAtt                                               <  1:581073/70‑1 (MQ=255)

                       aCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAtt                                               <  1:2627415/70‑1 (MQ=255)

                        cAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGc                                             <  1:3321416/71‑1 (MQ=255)

                         aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                             >  1:5369751/1‑54 (MQ=255)

                         aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgc                                                             >  1:1313452/1‑54 (MQ=255)

                         aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                                            <  1:4966100/71‑1 (MQ=255)

                         aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                                            <  1:1487587/71‑1 (MQ=255)

                          gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                                   <  1:678832/47‑1 (MQ=255)

                          gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                                           <  1:93396/71‑1 (MQ=255)

                              aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgata                                                         <  1:980012/53‑1 (MQ=255)

                               gCGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGgag                                       <  1:3349636/70‑1 (MQ=255)

                               gCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                          >  1:156403/1‑67 (MQ=255)

                                 ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                     >  1:2565244/1‑70 (MQ=255)

                                 ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                     >  1:702624/1‑70 (MQ=255)

                                   aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGcac                                                                 <  1:4887408/40‑1 (MQ=255)

                                   aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                           <  1:1567484/46‑1 (MQ=255)

                                   aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                                   <  1:518136/70‑1 (MQ=255)

                                    aCAAATCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                                   <  1:3619352/37‑1 (MQ=255)

                                    aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                           >  1:4716623/1‑45 (MQ=255)

                                    aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGgag                                       >  1:2561236/1‑65 (MQ=255)

                                     cAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAg                                                               >  1:2525723/1‑40 (MQ=255)

                                     cAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                          <  1:3740631/61‑1 (MQ=255)

                                      agttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCtt                               >  1:267579/4‑71 (MQ=255)

                                      aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                                   <  1:1256531/35‑1 (MQ=255)

                                      aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                                   <  1:2488766/35‑1 (MQ=255)

                                      aaaTCAGACTCTTTGCCCACCAGGCCTACcgccgc                                                                   <  1:5156539/35‑1 (MQ=255)

                                       aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                              >  1:3274186/1‑71 (MQ=255)

                                       aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                              >  1:753411/1‑71 (MQ=255)

                                        ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCg                                            <  1:1172219/55‑1 (MQ=255)

                                        ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttgc                             <  1:3223874/70‑3 (MQ=255)

                                        ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttg                              <  1:4195173/69‑2 (MQ=255)

                                        aTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCCATAGATTGCGGGGAGATCATCTTAc                             >  1:5497410/1‑71 (MQ=255)

                                         tCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCg                                            >  1:3907274/1‑55 (MQ=255)

                                         tCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAcc                            <  1:1158933/71‑1 (MQ=255)

                                          cAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttgc                             >  1:5118931/1‑67 (MQ=255)

                                          cAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGcc                            <  1:941088/70‑1 (MQ=255)

                                               tctTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                                   <  1:2796059/42‑1 (MQ=255)

                                               gctTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGgag                                       >  1:1170062/2‑54 (MQ=255)

                                                 tttGCCCACCAGGCCTACCGCCGCACAGGcgatagcgatag                                                  <  1:1825887/41‑1 (MQ=255)

                                                 tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcatc                                 >  1:4028601/1‑58 (MQ=255)

                                                 tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc                    >  1:5659472/1‑71 (MQ=255)

                                                 tttGCCCACCAGGACTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc                    >  1:4463872/1‑71 (MQ=255)

                                                   tGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                     >  1:4490880/1‑36 (MQ=255)

                                                    gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGa                       >  1:3194293/1‑65 (MQ=255)

                                                    gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                 >  1:5111582/1‑71 (MQ=255)

                                                    gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                 >  1:1941283/1‑71 (MQ=255)

                                                     cccACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                 <  1:2776999/70‑1 (MQ=255)

                                                      ccaccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCg               >  1:3093636/1‑71 (MQ=255)

                                                         ccaCGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                               <  1:4749875/52‑1 (MQ=255)

                                                          caGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg           <  1:1457428/71‑1 (MQ=255)

                                                            ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                     >  1:4750123/1‑43 (MQ=255)

                                                            ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa         >  1:4464874/1‑71 (MQ=255)

                                                               cTACCGCCGCACAGTCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTg      >  1:3802392/1‑71 (MQ=255)

                                                               cTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTg      >  1:4143537/1‑71 (MQ=255)

                                                                tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                               <  1:4616808/45‑1 (MQ=255)

                                                                tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCt                                <  1:5077731/44‑1 (MQ=255)

                                                                tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCt                                <  1:5234202/44‑1 (MQ=255)

                                                                 gtcgcggcGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTtgc    >  1:2997310/3‑71 (MQ=255)

                                                                  tcgcggcGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTtgct   >  1:2850094/2‑70 (MQ=255)

                                                                  ccgccgCACAGGCGATAGCGATAGATTGCGGGgag                                       >  1:671121/1‑35 (MQ=255)

                                                                  ccgccgCACAGGCGATAGCGATAGATTGCGGGGAGATCATCt                                >  1:5424943/1‑42 (MQ=255)

                                                                  ccgccgCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTggc    <  1:1358432/70‑1 (MQ=255)

                                                                    gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAc                             >  1:1967843/1‑43 (MQ=255)

                                                                    gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAc                             >  1:3246385/1‑43 (MQ=255)

                                                                    gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGt                         <  1:2815257/47‑1 (MQ=255)

                                                                    gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTggcgg  >  1:4962567/1‑70 (MQ=255)

AGGTGAAGATCAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGG  >  Exported/2562480‑2562617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`