BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,562,559	G→C	17.4%	I506M (ATC→ATG) ‡	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,562,559	0	G	C	17.4%	192.9 / 30.5	86	I506M (ATC→ATG) ‡	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base G (43/28); new base C (7/8); total (50/36)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

CAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAA  >  Exported/2562490‑2562627

cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                                     >  1:772871/1‑71 (MQ=255)

cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                                     >  1:3764316/1‑71 (MQ=255)

cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                                     >  1:4599883/1‑71 (MQ=255)

cAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                                     >  1:5518617/1‑71 (MQ=255)

   gCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatag                                                                  <  1:4509397/71‑1 (MQ=255)

    cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                                 <  1:3656059/71‑1 (MQ=255)

    cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                                 <  1:4318108/71‑1 (MQ=255)

    cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                                 <  1:1613557/71‑1 (MQ=255)

       tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgat                                                              <  1:1353602/71‑1 (MQ=255)

       tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                               <  1:5121931/70‑1 (MQ=255)

       tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                                    <  1:3451277/65‑1 (MQ=255)

        gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                               >  1:1374445/1‑69 (MQ=255)

        gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                                                      <  1:2046781/62‑1 (MQ=255)

        gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcg                                                                      <  1:1202237/62‑1 (MQ=255)

         tttGACAGTAAAGCTGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                                             <  1:2911125/70‑1 (MQ=255)

         tttGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGcgatagcgatag                                                            <  1:4068233/71‑1 (MQ=255)

           tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                                                          >  1:1335316/1‑71 (MQ=255)

            gACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                                                          >  1:630133/1‑70 (MQ=255)

            caCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                                                          >  1:4225911/2‑70 (MQ=255)

             aCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAtt                                                         <  1:581073/70‑1 (MQ=255)

             aCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAtt                                                         <  1:2627415/70‑1 (MQ=255)

              cAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGc                                                       <  1:3321416/71‑1 (MQ=255)

               aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                                                      <  1:1487587/71‑1 (MQ=255)

               aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                                                      <  1:4966100/71‑1 (MQ=255)

                gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                                                     <  1:93396/71‑1 (MQ=255)

                 tAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                                             <  1:4692752/62‑1 (MQ=255)

                   aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgggg                                                   <  1:1311113/70‑1 (MQ=255)

                    aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgata                                                                   <  1:980012/53‑1 (MQ=255)

                    aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                                    >  1:5267372/1‑68 (MQ=255)

                     gCGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCggg                                                    >  1:296299/1‑67 (MQ=255)

                     gCGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGgag                                                 <  1:3349636/70‑1 (MQ=255)

                     gCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                                    >  1:156403/1‑67 (MQ=255)

                      cGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                               <  1:3629734/71‑1 (MQ=255)

                       ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                               >  1:4808613/1‑70 (MQ=255)

                       ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                               >  1:5483102/1‑70 (MQ=255)

                        gAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                                             >  1:856635/1‑71 (MQ=255)

                         aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcga                                                                     <  1:1567484/46‑1 (MQ=255)

                         aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                                             <  1:518136/70‑1 (MQ=255)

                          aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGgag                                                 >  1:2561236/1‑65 (MQ=255)

                          aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcatc                                           >  1:4026579/1‑71 (MQ=255)

                          aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGAGATAGATTGCGGGgag                                                 >  1:740332/1‑65 (MQ=255)

                            agttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCtt                                         >  1:267579/4‑71 (MQ=255)

                             aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                                        >  1:753411/1‑71 (MQ=255)

                             aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                                        >  1:3274186/1‑71 (MQ=255)

                              ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCg                                                      <  1:1172219/55‑1 (MQ=255)

                              ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttgc                                       <  1:3223874/70‑3 (MQ=255)

                              ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttg                                        <  1:4195173/69‑2 (MQ=255)

                              ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttg                                        <  1:3259735/69‑2 (MQ=255)

                              aTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCCATAGATTGCGGGGAGATCATCTTAc                                       >  1:5497410/1‑71 (MQ=255)

                               tCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttg                                        <  1:5010779/69‑2 (MQ=255)

                               tCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAcc                                      <  1:1158933/71‑1 (MQ=255)

                                cAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGcc                                      <  1:941088/70‑1 (MQ=255)

                                 aGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCgg                                    >  1:1643765/1‑71 (MQ=255)

                                   aCTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGt                                   <  1:3799749/70‑1 (MQ=255)

                                     tctTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                                             <  1:2796059/42‑1 (MQ=255)

                                     gctTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGgag                                                 >  1:1170062/2‑54 (MQ=255)

                                       tttGCCCACCAGGCCTACCGCCGCACAGGcgatagcgatag                                                            <  1:1825887/41‑1 (MQ=255)

                                       tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcatc                                           >  1:4028601/1‑58 (MQ=255)

                                       tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc                              >  1:5659472/1‑71 (MQ=255)

                                       tttGCCCACCAGGCCTACCGC‑‑CACAGGCGATAGCGATAGATTGCGGGGAGatcatc                                           >  1:1884747/1‑56 (MQ=255)

                                       tttGCCCACCAGGACTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc                              >  1:4463872/1‑71 (MQ=255)

                                         tGCCCACCAGCCCTACCGCCGCACAGgcgatagcga                                                               >  1:2539022/1‑36 (MQ=255)

                                          gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGa                                 >  1:3194293/1‑65 (MQ=255)

                                          gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                           >  1:1941283/1‑71 (MQ=255)

                                          gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                           >  1:5111582/1‑71 (MQ=255)

                                           cccACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                           <  1:2776999/70‑1 (MQ=255)

                                            ccaccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCg                         >  1:3093636/1‑71 (MQ=255)

                                             caccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcat                                            <  1:1412731/51‑1 (MQ=255)

                                               ccaCGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                                         <  1:4749875/52‑1 (MQ=255)

                                                caGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg                     <  1:1457428/71‑1 (MQ=255)

                                                 aGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg                     >  1:3101389/1‑70 (MQ=255)

                                                  ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                               >  1:4750123/1‑43 (MQ=255)

                                                  ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa                   >  1:4464874/1‑71 (MQ=255)

                                                     cTACCGCCGCACAGTCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTg                >  1:3802392/1‑71 (MQ=255)

                                                     cTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTg                >  1:4143537/1‑71 (MQ=255)

                                                      tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                                         <  1:2330619/45‑1 (MQ=255)

                                                      tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                                         <  1:4616808/45‑1 (MQ=255)

                                                      tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                                         <  1:334080/45‑1 (MQ=255)

                                                      tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCt                                          <  1:5077731/44‑1 (MQ=255)

                                                      tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCt                                          <  1:5234202/44‑1 (MQ=255)

                                                       gtcgcggcGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTtgc              >  1:2997310/3‑71 (MQ=255)

                                                       gtcgcggcGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTtgc              >  1:4290080/3‑71 (MQ=255)

                                                        ccgccgCACAGGCGATAGCGATAGATTGCGGGgag                                                 >  1:671121/1‑35 (MQ=255)

                                                        ccgccgCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTggc              <  1:1358432/70‑1 (MQ=255)

                                                          gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAc                                       >  1:3246385/1‑43 (MQ=255)

                                                          gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGt                                   <  1:2815257/47‑1 (MQ=255)

                                                             gcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc        <  1:38867/71‑1 (MQ=255)

                                                               acaGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc        >  1:4993910/1‑69 (MQ=255)

                                                                caGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa                   >  1:3867121/1‑57 (MQ=255)

                                                                 aGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc    <  1:5458937/71‑1 (MQ=255)

                                                                 aGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc    <  1:4477575/71‑1 (MQ=255)

                                                                  ggCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggt                       >  1:1532170/1‑51 (MQ=255)

                                                                  ggCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc    >  1:1784924/1‑70 (MQ=255)

                                                                   gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa  >  1:3692022/1‑71 (MQ=255)

                                                                   gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa  >  1:3698900/1‑71 (MQ=255)

                                                                   gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa  <  1:4956448/71‑1 (MQ=255)

                                                                   gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa  <  1:3190675/71‑1 (MQ=255)

                                                                   gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa  >  1:937396/1‑71 (MQ=255)

                                                                     gatagcgatagATTGCGGGGAGATCATCTTACCGGTGa                                 <  1:4402374/38‑1 (MQ=255)

                                                                     gatagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa  >  1:3006952/1‑69 (MQ=255)

CAGGCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAA  >  Exported/2562490‑2562627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`