BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,562,561	T→C	17.4%	I506V (ATC→GTC) ‡	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,562,561	0	T	C	17.4%	184.7 / 30.7	86	I506V (ATC→GTC) ‡	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base T (43/28); new base C (7/8); total (50/36)
Fisher's exact test for biased strand distribution p-value = 3.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

Only 100 of 118 total aligned reads displayed.

GCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGA  >  Exported/2562493‑2562631

 cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                                     <  1:3044874/71‑1 (MQ=255)

 cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                                     <  1:3656059/71‑1 (MQ=255)

 cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                                     <  1:4318108/71‑1 (MQ=255)

 cTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagc                                                                     <  1:1613557/71‑1 (MQ=255)

    tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgat                                                                  <  1:1353602/71‑1 (MQ=255)

    tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                                   <  1:5121931/70‑1 (MQ=255)

    tgtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgat                                                                        <  1:3451277/65‑1 (MQ=255)

     gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                                   >  1:1374445/1‑69 (MQ=255)

     gtTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                                   >  1:169429/1‑69 (MQ=255)

      tttGACAGTAAAGCTGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                                                 <  1:2911125/70‑1 (MQ=255)

      tttGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                                   >  1:3311967/1‑68 (MQ=255)

        tGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                                                              >  1:1335316/1‑71 (MQ=255)

         gACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                                                              >  1:630133/1‑70 (MQ=255)

         caCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAt                                                              >  1:4225911/2‑70 (MQ=255)

          aCAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGAtt                                                             <  1:2627415/70‑1 (MQ=255)

           cAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGc                                                           <  1:3321416/71‑1 (MQ=255)

            aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                                                          <  1:1487587/71‑1 (MQ=255)

            aGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCg                                                          <  1:4966100/71‑1 (MQ=255)

             gTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgg                                                         <  1:93396/71‑1 (MQ=255)

              tAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                                                 <  1:4692752/62‑1 (MQ=255)

                aaGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCgggg                                                       <  1:1311113/70‑1 (MQ=255)

                 aGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                                        >  1:5267372/1‑68 (MQ=255)

                  gCGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCggg                                                        >  1:296299/1‑67 (MQ=255)

                  gCGGAACAGTTCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGgag                                                     <  1:3349636/70‑1 (MQ=255)

                  gCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                                        >  1:156403/1‑67 (MQ=255)

                   cGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                                   <  1:3629734/71‑1 (MQ=255)

                    ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                                   >  1:5483102/1‑70 (MQ=255)

                    ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                                   >  1:702624/1‑70 (MQ=255)

                    ggAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                                   >  1:4808613/1‑70 (MQ=255)

                      aaCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatca                                                 <  1:518136/70‑1 (MQ=255)

                       aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGgag                                                     >  1:2561236/1‑65 (MQ=255)

                       aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcatc                                               >  1:4026579/1‑71 (MQ=255)

                       aCAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGAGATAGATTGCGGGgag                                                     >  1:740332/1‑65 (MQ=255)

                        cAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCggg                                                        <  1:3740631/61‑1 (MQ=255)

                         agttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCtt                                             >  1:267579/4‑71 (MQ=255)

                          aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                                            >  1:3274186/1‑71 (MQ=255)

                          aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                                            >  1:753411/1‑71 (MQ=255)

                           ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCg                                                          <  1:1172219/55‑1 (MQ=255)

                           ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttgc                                           <  1:3223874/70‑3 (MQ=255)

                           ttCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttg                                            <  1:4195173/69‑2 (MQ=255)

                           aTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCCATAGATTGCGGGGAGATCATCTTAc                                           >  1:5497410/1‑71 (MQ=255)

                            tCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCg                                                          >  1:3907274/1‑55 (MQ=255)

                            tCAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttg                                            <  1:5010779/69‑2 (MQ=255)

                            tCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAcc                                          <  1:1158933/71‑1 (MQ=255)

                             cAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCttgc                                           >  1:5118931/1‑67 (MQ=255)

                             cAGATTCTCGGCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGcc                                          <  1:941088/70‑1 (MQ=255)

                              aGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCgg                                        >  1:1643765/1‑71 (MQ=255)

                                  tctTTGCCCACCAGGCCTACCGCCGCACAGgcgatagcgata                                                                 <  1:2796059/42‑1 (MQ=255)

                                  gctTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGgag                                                     >  1:1170062/2‑54 (MQ=255)

                                    tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcatc                                               >  1:4028601/1‑58 (MQ=255)

                                    tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc                                  >  1:5659472/1‑71 (MQ=255)

                                    tttGCCCACCAGGCCTACCGC‑‑CACAGGCGATAGCGATAGATTGCGGGGAGatcatc                                               >  1:1884747/1‑56 (MQ=255)

                                    tttGCCCACCAGGACTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc                                  >  1:4463872/1‑71 (MQ=255)

                                      tGCCCACCAGGCCTACCGCCGCACAGgcgatagcga                                                                   >  1:4490880/1‑36 (MQ=255)

                                      tGCCCACCAGCCCTACCGCCGCACAGgcgatagcga                                                                   >  1:2539022/1‑36 (MQ=255)

                                       gCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGccgc                               <  1:4157060/71‑3 (MQ=255)

                                       gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGa                                     >  1:3194293/1‑65 (MQ=255)

                                       gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                               >  1:1941283/1‑71 (MQ=255)

                                       gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                               >  1:5111582/1‑71 (MQ=255)

                                        cccACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                               <  1:2776999/70‑1 (MQ=255)

                                         ccaccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCg                             >  1:3093636/1‑71 (MQ=255)

                                          caccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGatcat                                                <  1:1412731/51‑1 (MQ=255)

                                            ccaCGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                                             <  1:4749875/52‑1 (MQ=255)

                                             caGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg                         <  1:1457428/71‑1 (MQ=255)

                                               ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                                   >  1:5589699/1‑43 (MQ=255)

                                               ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGat                                                   >  1:4750123/1‑43 (MQ=255)

                                               ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa                       >  1:4464874/1‑71 (MQ=255)

                                                  cTACCGCCGCACAGTCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTg                    >  1:3802392/1‑71 (MQ=255)

                                                  cTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTg                    >  1:4143537/1‑71 (MQ=255)

                                                   tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                                             <  1:4616808/45‑1 (MQ=255)

                                                   tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                                             <  1:334080/45‑1 (MQ=255)

                                                   tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCtt                                             <  1:2330619/45‑1 (MQ=255)

                                                   tACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCt                                              <  1:5234202/44‑1 (MQ=255)

                                                    gtcgcggcGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTtgc                  >  1:4290080/3‑71 (MQ=255)

                                                    gtcgcggcGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTtgc                  >  1:2997310/3‑71 (MQ=255)

                                                     ccgccgCACAGGCGATAGCGATAGATTGCGGGgag                                                     >  1:671121/1‑35 (MQ=255)

                                                       gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAc                                           >  1:3246385/1‑43 (MQ=255)

                                                       gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAc                                           >  1:1967843/1‑43 (MQ=255)

                                                       gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGt                                       <  1:2815257/47‑1 (MQ=255)

                                                       gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTggcgg                >  1:4962567/1‑70 (MQ=255)

                                                          gcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc            <  1:905535/71‑1 (MQ=255)

                                                          gcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc            <  1:38867/71‑1 (MQ=255)

                                                            acaGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc            >  1:4993910/1‑69 (MQ=255)

                                                             caGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa                       >  1:1403883/1‑57 (MQ=255)

                                                             caGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa                       >  1:3867121/1‑57 (MQ=255)

                                                              aGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc        <  1:5458937/71‑1 (MQ=255)

                                                              aGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc        <  1:4477575/71‑1 (MQ=255)

                                                               ggCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggt                           >  1:1532170/1‑51 (MQ=255)

                                                               ggCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc        >  1:1784924/1‑70 (MQ=255)

                                                                gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa      <  1:3190675/71‑1 (MQ=255)

                                                                gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa      >  1:3198310/1‑71 (MQ=255)

                                                                gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa      >  1:3692022/1‑71 (MQ=255)

                                                                gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa      >  1:937396/1‑71 (MQ=255)

                                                                gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa      >  1:3698900/1‑71 (MQ=255)

                                                                  gatagcgatagATTGCGGGGAGATCATCTTACCGGTGa                                     <  1:4402374/38‑1 (MQ=255)

                                                                  gatagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa      >  1:3006952/1‑69 (MQ=255)

                                                                   atagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACAggtggt                        <  1:4902000/50‑1 (MQ=255)

                                                                    tagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa      >  1:760940/1‑67 (MQ=255)

                                                                    tagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGa  <  1:4985427/71‑1 (MQ=255)

                                                                    tagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGa  <  1:933193/71‑1 (MQ=255)

GCTGTGTTTGACAGTAAAGCGGAACAAATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGA  >  Exported/2562493‑2562631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`