BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,562,589	A→G	7.8%	G496G (GGT→GGC)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,562,589	0	A	G	7.8%	290.3 / 10.1	102	G496G (GGT→GGC)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand): ref base A (46/48); new base G (4/4); total (50/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

AATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCGG  >  Exported/2562519‑2562659

aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                                                                        >  1:753411/1‑71 (MQ=255)

aaTCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTa                                                                        >  1:3274186/1‑71 (MQ=255)

  tCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAcc                                                                      <  1:1158933/71‑1 (MQ=255)

          tttGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc                                                              >  1:5659472/1‑71 (MQ=255)

          tttGCCCACCAGGACTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGc                                                              >  1:4463872/1‑71 (MQ=255)

             gCCCACCATGCCCGTCGCGGCGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGccgc                                                           <  1:4157060/71‑3 (MQ=255)

             gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGa                                                                 >  1:3194293/1‑65 (MQ=255)

             gCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                                                           >  1:1941283/1‑71 (MQ=255)

              cccACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCAc                                                           <  1:2776999/70‑1 (MQ=255)

               ccaccaGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCg                                                         >  1:3093636/1‑71 (MQ=255)

                   caGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg                                                     <  1:1457428/71‑1 (MQ=255)

                    aGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtgg                                                     >  1:3101389/1‑70 (MQ=255)

                     ggCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa                                                   >  1:4464874/1‑71 (MQ=255)

                        cTACCGCCGCACAGTCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTg                                                >  1:3802392/1‑71 (MQ=255)

                        cTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTg                                                >  1:4143537/1‑71 (MQ=255)

                          gtcgcggcGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTtgc                                              >  1:4290080/3‑71 (MQ=255)

                          gtcgcggcGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTtgc                                              >  1:2997310/3‑71 (MQ=255)

                           tcgcggcGCAGGCCACGGCGATAGATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTtgct                                             >  1:2850094/2‑70 (MQ=255)

                           ccgccgCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTggc                                              <  1:1358432/70‑1 (MQ=255)

                            cgccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTgg                                               <  1:4372768/68‑1 (MQ=255)

                             gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAc                                                                       >  1:1967843/1‑43 (MQ=255)

                             gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTAc                                                                       >  1:3246385/1‑43 (MQ=255)

                             gccgcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTggcgg                                            >  1:4962567/1‑70 (MQ=255)

                                gcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc                                        <  1:905535/71‑1 (MQ=255)

                                gcACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc                                        <  1:38867/71‑1 (MQ=255)

                                  acaGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAAc                                        >  1:4993910/1‑69 (MQ=255)

                                   caGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa                                                   >  1:1403883/1‑57 (MQ=255)

                                   caGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTa                                                   >  1:3867121/1‑57 (MQ=255)

                                    aGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc                                    <  1:5458937/71‑1 (MQ=255)

                                    aGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc                                    <  1:4477575/71‑1 (MQ=255)

                                     ggCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCggt                                                       >  1:1532170/1‑51 (MQ=255)

                                     ggCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc                                    >  1:1784924/1‑70 (MQ=255)

                                      gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGc                                    <  1:3603062/69‑1 (MQ=255)

                                      gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                  >  1:3692022/1‑71 (MQ=255)

                                      gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                  >  1:3698900/1‑71 (MQ=255)

                                      gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                  <  1:3190675/71‑1 (MQ=255)

                                      gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                  >  1:3198310/1‑71 (MQ=255)

                                      gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                  >  1:937396/1‑71 (MQ=255)

                                      gcgatagcgataGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                  <  1:4956448/71‑1 (MQ=255)

                                        gatagcgatagATTGCGGGGAGATCATCTTACCGGTGa                                                                 <  1:4402374/38‑1 (MQ=255)

                                        gatagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                  >  1:3006952/1‑69 (MQ=255)

                                         atagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACAggtggt                                                    <  1:4902000/50‑1 (MQ=255)

                                          tagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                  >  1:760940/1‑67 (MQ=255)

                                          tagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGa                              <  1:4985427/71‑1 (MQ=255)

                                          tagcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGa                              <  1:933193/71‑1 (MQ=255)

                                          cggcgatagATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTTGCTGCCAc                                        >  1:3575025/3‑61 (MQ=255)

                                           agcgatagATTGCGGGGAGATCATCTTACCGGTGa                                                                 >  1:322529/1‑35 (MQ=255)

                                           agcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtg                                                      >  1:4864617/1‑46 (MQ=255)

                                           agcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCggtg                                                      >  1:884592/1‑46 (MQ=255)

                                           agcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                  >  1:2991359/1‑66 (MQ=255)

                                           agcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCaa                                  >  1:1040689/1‑66 (MQ=255)

                                            gcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGAt                             <  1:3589432/70‑1 (MQ=255)

                                            gcgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGAt                             <  1:3799621/70‑1 (MQ=255)

                                             cgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATc                            <  1:4468703/70‑1 (MQ=255)

                                             cgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                           <  1:2402859/71‑1 (MQ=255)

                                             cgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                           <  1:2206542/71‑1 (MQ=255)

                                             cgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                           <  1:3975860/71‑1 (MQ=255)

                                             cgatagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                           <  1:732248/71‑1 (MQ=255)

                                               atagATTGCGGGGAGATCATCTTGCCAGTTACGCCGCCgctggtgt                                                  <  1:5347731/46‑3 (MQ=255)

                                               atagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCaga                         <  1:4903605/71‑1 (MQ=255)

                                               atagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                           <  1:4125777/69‑1 (MQ=255)

                                               atagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                           <  1:2099888/69‑1 (MQ=255)

                                                tagATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                           >  1:3856092/1‑68 (MQ=255)

                                                 agATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCagag                        >  1:5168142/1‑70 (MQ=255)

                                                 agATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAgaga                       >  1:4826657/1‑71 (MQ=255)

                                                  gATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAgaga                       >  1:1835337/1‑70 (MQ=255)

                                                  gATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAgaga                       >  1:4528684/1‑70 (MQ=255)

                                                   aTTGCGGGGAGATCATCTTGCCAGTTACGCCGCCGCTGGTGTTTGCTGCCACCAGCa                                   <  1:4719050/57‑1 (MQ=255)

                                                   aTTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCa                           <  1:521037/65‑1 (MQ=255)

                                                   aTTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACg                     >  1:2290931/1‑71 (MQ=255)

                                                      gCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGcc                   <  1:4432399/70‑1 (MQ=255)

                                                          ggAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAAttt              >  1:3305907/1‑71 (MQ=255)

                                                          ggAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAAttt              <  1:1706535/71‑1 (MQ=255)

                                                          ggAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAAttt              <  1:2742185/71‑1 (MQ=255)

                                                          ggAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAAttt              <  1:5347637/71‑1 (MQ=255)

                                                          ggAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAAttt              <  1:2518998/71‑1 (MQ=255)

                                                          ggAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACAAGCAACAGATCAGAGACGCCAAttt              <  1:4530105/71‑1 (MQ=255)

                                                           gagaTCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttg             <  1:725154/71‑1 (MQ=255)

                                                           gagaTCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttg             <  1:5653578/71‑1 (MQ=255)

                                                           gagaTCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttg             <  1:5386954/71‑1 (MQ=255)

                                                            agaTCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgt            <  1:3876918/71‑1 (MQ=255)

                                                            agaTCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgt            <  1:1250462/71‑1 (MQ=255)

                                                              atcatcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGcc                   >  1:1369123/1‑62 (MQ=255)

                                                              atcatcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGc                    <  1:5144430/61‑1 (MQ=255)

                                                               tcatcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACg                     <  1:1897474/59‑1 (MQ=255)

                                                               tcatcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACg                     <  1:2444955/59‑1 (MQ=255)

                                                               tcatcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACg                     <  1:1601814/59‑1 (MQ=255)

                                                                catcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgttg          <  1:2369473/69‑1 (MQ=255)

                                                                catcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTtgttgt         >  1:5348102/1‑70 (MQ=255)

                                                                catcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTtgtg        >  1:1459403/1‑71 (MQ=255)

                                                                  tcTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAAttt              <  1:5438080/63‑1 (MQ=255)

                                                                   cTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttgttg          >  1:4879143/1‑66 (MQ=255)

                                                                   cTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGtgc       >  1:2737973/1‑69 (MQ=255)

                                                                    ttACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGtgctgc    >  1:411249/1‑71 (MQ=255)

                                                                     tACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATttg             >  1:3090777/1‑61 (MQ=255)

                                                                      aCCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGtgctgc    <  1:2011742/69‑1 (MQ=255)

                                                                      aCCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGtgctgc    <  1:514004/69‑1 (MQ=255)

                                                                      aCCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGtgct      <  1:2085545/67‑1 (MQ=255)

                                                                      aCCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGtgct      <  1:2268682/67‑1 (MQ=255)

                                                                      aCCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCgg  >  1:1921218/1‑71 (MQ=255)

AATCAGACTCTTTGCCCACCAGGCCTACCGCCGCACAGGCGATAGCGATAGATTGCGGGGAGATCATCTTACCGGTGACGCCACCGGTGGTATTGGCGGCAACCAGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCGG  >  Exported/2562519‑2562659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`