Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,562,691	T→G	17.7%	S462S (TCA→TCC)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,562,691	0	T	G	17.7%	152.5 / 38.4	85	S462S (TCA→TCC)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand):  ref base T (23/47);  new base G (6/9);  total (29/56)
Fisher's exact test for biased strand distribution p-value = 7.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

CCAGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGA  >  Exported/2562621‑2562760                                                                       |                                                                      ccAGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACATGGCGTTAGAt                                                                       <  1:3856125/71‑1 (MQ=255)  cAGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGAt                                                                       >  1:5015979/1‑70 (MQ=255)   aGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGAt                                                                       >  1:1342810/1‑69 (MQ=255)   aGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGa                                                                     <  1:5227378/71‑1 (MQ=255)   aGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGa                                                                     <  1:3470231/71‑1 (MQ=255)     cAACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGTGGCGAACAGGGCGTTAGATGAgg                                                                   >  1:4989558/1‑71 (MQ=255)     cAACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAgg                                                                   <  1:779616/71‑1 (MQ=255)     cAACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAgg                                                                   <  1:3487730/71‑1 (MQ=255)     cAACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAgg                                                                   >  1:2124593/1‑71 (MQ=255)      aaCAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAgg                                                                   <  1:4421233/70‑1 (MQ=255)      aaCAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGt                                                                  <  1:1714426/71‑1 (MQ=255)      aaCAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGt                                                                  <  1:4222068/71‑1 (MQ=255)       aCAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTa                                                                 >  1:21861/1‑71 (MQ=255)        cAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTAt                                                                >  1:384786/1‑71 (MQ=255)         aGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATc                                                               >  1:859256/1‑71 (MQ=255)          tgtCAGAGACGTTGATTTGCTGCGCCGTGGTCGATTGCAGTGAACCAAACAGGGCGTTAGAGGAGGTGTcc                                                              <  1:2917850/69‑1 (MQ=255)          tgtCAGAGACGTTGATTTGCTGCGCCGTGGTCGATTGCAGTGAACCAAACAGGGCGTTAGAGGAGGTGTcc                                                              <  1:1458391/69‑1 (MQ=255)          tgtCAGAGACGTTGATTTGCTGCGCCGTGGTCGATTGCAGTGAACCAAACAGGGCGTTAGAGGAGGTGTcc                                                              <  1:1441043/69‑1 (MQ=255)          tgtCAGAGACGTTGATTTGCTGCGCCGTGGTCGATTGCAGTGAACCAAACAGGGCGTTAGAGGAGGTGTcc                                                              <  1:3939528/69‑1 (MQ=255)          gATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATcc                                                              <  1:781401/71‑1 (MQ=255)          gATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATc                                                               <  1:4643528/70‑1 (MQ=255)            tCAGAGACGTTGATTTGCTGCGCCGTGGTCGATTGCAGTGAACCAAACAGGGCGTTAGAGGAGGTGTCCg                                                             <  1:5379648/70‑1 (MQ=255)            tCAGAGACGTTGATTTGCTGCGCCGTGGTCGATTGCAGTGAACCAAACAGGGCGTTAGAGGAGGTGTCCg                                                             <  1:5636860/70‑1 (MQ=255)             cAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGAc                                                           >  1:2162155/1‑71 (MQ=255)              agagACGTTGATTTGCTGCGCCGTGGTCGATTGCAGTGAACCAAACAGGGCGTTAGAGGAGGTGTCCGagc                                                          >  1:3657939/1‑69 (MQ=255)              agagACGCCAATTTGTTGTGCTGCGGTGCCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGAcc                                                          >  1:4155344/1‑71 (MQ=255)                agaCGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCg                                                        <  1:4083833/71‑1 (MQ=255)                  aCGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGt                                                      <  1:2688111/71‑1 (MQ=255)                    gCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTc                                                     <  1:4373999/70‑1 (MQ=255)                     ccAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                   <  1:5038296/71‑1 (MQ=255)                     ccAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                   <  1:5506855/71‑1 (MQ=255)                     ccAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                   <  1:2247352/71‑1 (MQ=255)                      cAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                  <  1:1007519/71‑1 (MQ=255)                      cAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                   >  1:1724878/1‑70 (MQ=255)                       aaTTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                  >  1:3127479/1‑70 (MQ=255)                       aaTTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                  >  1:4393023/1‑70 (MQ=255)                        aTTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGaa                                                <  1:2489262/71‑1 (MQ=255)                         tttGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGaa                                                >  1:5026726/1‑70 (MQ=255)                           tgttgtGCTGCGGTGGCTTGCAGCGCGGCTAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATa                                              <  1:281282/70‑1 (MQ=255)                           tgttgtGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATa                                              <  1:2174298/70‑1 (MQ=255)                           tgttgtGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATAc                                             >  1:4025032/1‑71 (MQ=255)                                tgctgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCcca                                        <  1:2720917/71‑1 (MQ=255)                                 gctgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                   >  1:4231222/1‑59 (MQ=255)                                 gctgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                   >  1:4986613/1‑59 (MQ=255)                                   tgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                  <  1:379085/58‑1 (MQ=255)                                   tgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                  <  1:552893/58‑1 (MQ=255)                                   tgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGaa                                                <  1:1714504/60‑1 (MQ=255)                                   tgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGaa                                                <  1:829364/60‑1 (MQ=255)                                    gcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCccagc                                      >  1:1995086/1‑69 (MQ=255)                                       gTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAgg                                                                   <  1:5157301/37‑1 (MQ=255)                                       gTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAgg                                                                   <  1:1126464/37‑1 (MQ=255)                                       gTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAgg                                                                   <  1:4147606/37‑1 (MQ=255)                                       gTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCagccagcc                                 >  1:3022863/1‑71 (MQ=255)                                        tGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGt                                                      <  1:3863161/49‑1 (MQ=255)                                        tGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCagccagcc                                 <  1:1658321/70‑1 (MQ=255)                                         ggCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCagccagcc                                 >  1:3255476/1‑69 (MQ=255)                                            ttGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGAcc                                                          <  1:5156631/41‑1 (MQ=255)                                              gCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGa                            <  1:915637/69‑1 (MQ=255)                                               cAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAAc                          <  1:4768465/70‑1 (MQ=255)                                               cAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCAGTCAGGAATACCCCCAGCCAGCCGAGGAAc                          <  1:2596432/70‑1 (MQ=255)                                                aGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACgg                        >  1:383742/1‑71 (MQ=255)                                                    cggcgAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCagccagcc                                 <  1:324851/58‑1 (MQ=255)                                                    cggcgAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCg                      <  1:5575591/69‑1 (MQ=255)                                                      gcgAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCcca                                        >  1:3175738/1‑49 (MQ=255)                                                      gcgAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaa                  <  1:2752940/71‑1 (MQ=255)                                                        gAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaaga                <  1:699560/71‑1 (MQ=255)                                                        aaaCAGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGaa                                                >  1:2414073/2‑39 (MQ=25)                                                         aaCAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                  <  1:1439776/36‑1 (MQ=255)                                                         aaCAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                  <  1:5387027/36‑1 (MQ=255)                                                         aaCAGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAgaagaa               <  1:1829411/71‑1 (MQ=255)                                                         aaCAGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAgaagaa               <  1:1830777/71‑1 (MQ=255)                                                          aCAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGaagaag              >  1:3731184/1‑71 (MQ=255)                                                          aCAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGaagaag              >  1:170310/1‑71 (MQ=255)                                                            aGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt            <  1:1533219/71‑1 (MQ=255)                                                            aGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt            <  1:4194809/71‑1 (MQ=255)                                                            aGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACgg                        >  1:4234613/1‑59 (MQ=255)                                                            aGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAgaagaa               >  1:1320443/1‑68 (MQ=255)                                                             gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTg           <  1:3965777/71‑1 (MQ=255)                                                             gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTg           <  1:260290/71‑1 (MQ=255)                                                             gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCACCAGCCAGCCGAGGAACGGCGagaaga                <  1:4815674/66‑1 (MQ=255)                                                               gCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaa         >  1:4260842/1‑71 (MQ=255)                                                               gCGTTAGAGGAGGTGTCCGAGCCGCTAAGCAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaa         >  1:3230097/1‑71 (MQ=255)                                                                  ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaag                 <  1:5207988/60‑1 (MQ=255)                                                                  ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAAt        <  1:5457942/69‑1 (MQ=255)                                                                  ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAAt        <  1:3331589/69‑1 (MQ=255)                                                                  ttAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaacat      <  1:4839846/71‑4 (MQ=255)                                                                    agagGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACggt                       >  1:4210752/1‑51 (MQ=19)                                                                    aGATGAGGTATCCGACCCGGTCAGGAATACCCCcagccagc                                  >  1:4126596/1‑41 (MQ=255)                                                                    aGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCAt    >  1:3162993/1‑71 (MQ=255)                                                                       tGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGa  <  1:1856228/70‑1 (MQ=255)                                                                       |                                                                      CCAGCAACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGA  >  Exported/2562621‑2562760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑