Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,562,697	A→G	22.1%	D460D (GAT→GAC)	lldP ←	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,562,697	0	A	G	22.1%	143.0 / 36.4	77	D460D (GAT→GAC)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand):  ref base A (21/39);  new base G (8/9);  total (29/48)
Fisher's exact test for biased strand distribution p-value = 4.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACC  >  Exported/2562627‑2562762                                                                       |                                                                  aCAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTa                                                                   >  1:21861/1‑71 (MQ=255)  cAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTAt                                                                  >  1:384786/1‑71 (MQ=255)   aGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATc                                                                 >  1:859256/1‑71 (MQ=255)    tgtCAGAGACGTTGATTTGCTGCGCCGTGGTCGATTGCAGTGAACCAAACAGGGCGTTAGAGGAGGTGTcc                                                                <  1:2917850/69‑1 (MQ=255)    tgtCAGAGACGTTGATTTGCTGCGCCGTGGTCGATTGCAGTGAACCAAACAGGGCGTTAGAGGAGGTGTcc                                                                <  1:1441043/69‑1 (MQ=255)    tgtCAGAGACGTTGATTTGCTGCGCCGTGGTCGATTGCAGTGAACCAAACAGGGCGTTAGAGGAGGTGTcc                                                                <  1:1458391/69‑1 (MQ=255)    tgtCAGAGACGTTGATTTGCTGCGCCGTGGTCGATTGCAGTGAACCAAACAGGGCGTTAGAGGAGGTGTcc                                                                <  1:3939528/69‑1 (MQ=255)    gATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATcc                                                                <  1:781401/71‑1 (MQ=255)    gATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATc                                                                 <  1:4643528/70‑1 (MQ=255)      tCAGAGACGTTGATTTGCTGCGCCGTGGTCGATTGCAGTGAACCAAACAGGGCGTTAGAGGAGGTGTCCg                                                               <  1:5636860/70‑1 (MQ=255)      tCAGAGACGTTGATTTGCTGCGCCGTGGTCGATTGCAGTGAACCAAACAGGGCGTTAGAGGAGGTGTCCg                                                               <  1:5379648/70‑1 (MQ=255)       cAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGAc                                                             >  1:2162155/1‑71 (MQ=255)        agagACGTTGATTTGCTGCGCCGTGGTCGATTGCAGTGAACCAAACAGGGCGTTAGAGGAGGTGTCCGagc                                                            >  1:3657939/1‑69 (MQ=255)        agagACGCCAATTTGTTGTGCTGCGGTGCCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGAcc                                                            >  1:4155344/1‑71 (MQ=255)          agaCGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCg                                                          <  1:4083833/71‑1 (MQ=255)            aCGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGt                                                        <  1:2688111/71‑1 (MQ=255)              gCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTc                                                       <  1:4373999/70‑1 (MQ=255)               ccAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                     <  1:5506855/71‑1 (MQ=255)               ccAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                     <  1:2247352/71‑1 (MQ=255)               ccAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                     <  1:5038296/71‑1 (MQ=255)                cAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                    <  1:1007519/71‑1 (MQ=255)                cAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                     >  1:1724878/1‑70 (MQ=255)                 aaTTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                    >  1:4393023/1‑70 (MQ=255)                 aaTTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                    >  1:3127479/1‑70 (MQ=255)                  aTTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGaa                                                  <  1:2489262/71‑1 (MQ=255)                   tttGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGaa                                                  >  1:5026726/1‑70 (MQ=255)                     tgttgtGCTGCGGTGGCTTGCAGCGCGGCTAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATa                                                <  1:281282/70‑1 (MQ=255)                     tgttgtGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATa                                                <  1:2174298/70‑1 (MQ=255)                     tgttgtGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATAc                                               >  1:4025032/1‑71 (MQ=255)                          tgctgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCcca                                          <  1:2720917/71‑1 (MQ=255)                           gctgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                     >  1:4986613/1‑59 (MQ=255)                           gctgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAg                                                     >  1:4231222/1‑59 (MQ=255)                             tgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                    <  1:379085/58‑1 (MQ=255)                             tgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                    <  1:552893/58‑1 (MQ=255)                             tgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGaa                                                  <  1:1714504/60‑1 (MQ=255)                             tgcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGaa                                                  <  1:829364/60‑1 (MQ=255)                              gcGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCccagc                                        >  1:1995086/1‑69 (MQ=255)                                 gTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCagccagcc                                   >  1:3022863/1‑71 (MQ=255)                                  tGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGt                                                        <  1:3863161/49‑1 (MQ=255)                                  tGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCagccagcc                                   <  1:1658321/70‑1 (MQ=255)                                   ggCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCagccagcc                                   >  1:3255476/1‑69 (MQ=255)                                      ttGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGAcc                                                            <  1:5156631/41‑1 (MQ=255)                                        gCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGa                              <  1:915637/69‑1 (MQ=255)                                         cAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAAc                            <  1:4768465/70‑1 (MQ=255)                                         cAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCAGTCAGGAATACCCCCAGCCAGCCGAGGAAc                            <  1:2596432/70‑1 (MQ=255)                                          aGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACgg                          >  1:383742/1‑71 (MQ=255)                                              cggcgAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCagccagcc                                   <  1:324851/58‑1 (MQ=255)                                              cggcgAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCg                        <  1:5575591/69‑1 (MQ=255)                                                gcgAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCcca                                          >  1:3175738/1‑49 (MQ=255)                                                gcgAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaa                    <  1:2752940/71‑1 (MQ=255)                                                  gAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaaga                  <  1:699560/71‑1 (MQ=255)                                                  aaaCAGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGaa                                                  >  1:2414073/2‑39 (MQ=25)                                                   aaCAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                    <  1:1439776/36‑1 (MQ=255)                                                   aaCAGGGCGTTAGATGAGGTATCCGACCCGGTCAgg                                                    <  1:5387027/36‑1 (MQ=255)                                                   aaCAGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAgaagaa                 <  1:1829411/71‑1 (MQ=255)                                                   aaCAGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAgaagaa                 <  1:1830777/71‑1 (MQ=255)                                                    aCAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGaagaag                >  1:170310/1‑71 (MQ=255)                                                    aCAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGaagaag                >  1:3731184/1‑71 (MQ=255)                                                      aGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt              <  1:1533219/71‑1 (MQ=255)                                                      aGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt              <  1:4194809/71‑1 (MQ=255)                                                      aGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACgg                          >  1:4234613/1‑59 (MQ=255)                                                      aGGGCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAgaagaa                 >  1:1320443/1‑68 (MQ=255)                                                       gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTg             <  1:260290/71‑1 (MQ=255)                                                       gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTg             <  1:3965777/71‑1 (MQ=255)                                                       gggCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCACCAGCCAGCCGAGGAACGGCGagaaga                  <  1:4815674/66‑1 (MQ=255)                                                         gCGTTAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaa           >  1:4260842/1‑71 (MQ=255)                                                         gCGTTAGAGGAGGTGTCCGAGCCGCTAAGCAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaa           >  1:3230097/1‑71 (MQ=255)                                                            ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGagaag                   <  1:5207988/60‑1 (MQ=255)                                                            ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAAt          <  1:5457942/69‑1 (MQ=255)                                                            ttAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAAt          <  1:3331589/69‑1 (MQ=255)                                                            ttAGAGGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGaacat        <  1:4839846/71‑4 (MQ=255)                                                              agagGAGGTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACggt                         >  1:4210752/1‑51 (MQ=19)                                                              aGATGAGGTATCCGACCCGGTCAGGAATACCCCcagccagc                                    >  1:4126596/1‑41 (MQ=255)                                                              aGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCAt      >  1:3162993/1‑71 (MQ=255)                                                                 tGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGa    <  1:1856228/70‑1 (MQ=255)                                                                  gAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGAcc  >  1:2169980/1‑71 (MQ=255)                                                                   aGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGt              <  1:3951219/58‑1 (MQ=255)                                                                    ggTGTCCGAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGagaa                    >  1:5360147/1‑51 (MQ=19)                                                                       |                                                                  ACAGATCAGAGACGCCAATTTGTTGTGCTGCGGTGGCTTGCAGCGCGGCGAACAGGGCGTTAGATGAGGTATCCGACCCGGTCAGGAATACCCCCAGCCAGCCGAGGAACGGCGAGAAGAAGGTGAATGCATGACC  >  Exported/2562627‑2562762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑